The serotonin 5-HT2 receptor-phospholipase C system inhibits the induction of long-term potentiation in the rat visual cortex

Yoshikuni Edagawa, Hiroshi Saito, Kazuho Abe

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The effect of serotonin 5-HT2 receptor stimulation on long-term potentiation (LTP) in the primary visual cortex was investigated by using rat brain slices in vitro. Field potentials evoked by stimulation of layer IV were recorded in layer II/III. The 5-HT2 receptor agonist 1-(2,5-dimethyl-4-iodophenyl)-2-aminopropane (DOI) did not affect baseline synaptic potentials evoked by single-pulse test stimulation, but significantly inhibited the induction of LTP in a concentration-dependent manner (0.1-10 μM). The LTP-inhibiting effect of DOI (10 μM) was blocked by the 5-HT(2,7) receptor antagonist ritanserin (10 μM), but not by the 5-HT(1A) receptor antagonist NAN-190 (10 μM) nor by the 5-HT(2,7) receptor antagonist MDL72222 (10 μM). The inhibitory effect of DOI was also blocked by the phospholipase C inhibitor U73122, but not by its inactive analogue U73343. These results suggest that visual cortex LTP is inhibited by activation of the 5-HT2 receptor-phospholipase C system. In addition, the LTP-inhibiting effect of DOI was abolished by the presence of the GABA(A) receptor antagonist bicuculline (10 μM), suggesting that 5-HT2 receptor-mediated inhibition of visual cortex LTP is dependent on GABAergic inhibition.

Original languageEnglish
Pages (from-to)1391-1396
Number of pages6
JournalEuropean Journal of Neuroscience
Volume12
Issue number4
DOIs
Publication statusPublished - 2000
Externally publishedYes

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Serotonin 5-HT2 Receptors
Long-Term Potentiation
Type C Phospholipases
Visual Cortex
Serotonin 5-HT2 Receptor Agonists
Ritanserin
GABA-A Receptor Antagonists
Synaptic Potentials
Receptor, Serotonin, 5-HT1A
Bicuculline
Evoked Potentials
Brain

Keywords

  • 5-HT receptor
  • GABA(A) receptor
  • Long-term potentiation
  • Phospholipase C
  • Serotonin
  • Visual cortex

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

The serotonin 5-HT2 receptor-phospholipase C system inhibits the induction of long-term potentiation in the rat visual cortex. / Edagawa, Yoshikuni; Saito, Hiroshi; Abe, Kazuho.

In: European Journal of Neuroscience, Vol. 12, No. 4, 2000, p. 1391-1396.

Research output: Contribution to journalArticle

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