The site of photoinhibition in leaves of Cucumis sativus L. at low temperatures is photosystem I, not photosystem II

Ichiro Terashima, Sachiko Funayama, Kintake Sonoike

Research output: Contribution to journalArticle

207 Citations (Scopus)

Abstract

Maximum quantum yields (QY) of photosynthetic electron flows through PSI and PSII were separately assessed in thylakoid membranes isolated from leaves of Cucumis sativus L. (cucumber) that had been chilled in various ways. The QY(PSI) in the thylakoids prepared from the leaves treated at 4° C in moderate light at 220 μmol quanta·m-2·s-1 (400-700 nm) for 5 h, was about 20-30% of that in the thylakoids prepared from untreated leaves, while QY(PSII) decreased, at most, by 20% in response to the same treatment. The decrease in QY(PSI) was observed only when the leaves were chilled at temperatures below 10° C, while such a marked temperature dependency was not observed for the decrease in QY(PSII). In the chilling treatment at 4° C for 5 h, the quantum flux density that was required to induce 50% loss of QY (PSI) was ca. 50 umol quanta·m-2·s-1. When the chilling treatment at 4° C in the light was conducted in an atmosphere of N2, photoinhibition of PSI was largely suppressed, while the damage to PSII was somewhat enhanced. The ferricyanide-oxidised minus ascorbate-reduced difference spectra and the light-induced absorbance changes at 700 nm obtained with the thylakoid suspension, indicated the loss of P700 to extents that corresponded to the decreases in QY(PSI). Accordingly, the decreases in QY(PSI) can largely be attributed to destruction of the PSI reaction centre itself. These results clearly show that, at least in cucumber, a typical chillingsensitive plant, PSI is much more susceptible to aerobic photoinhibition than PSII.

Original languageEnglish
Pages (from-to)300-306
Number of pages7
JournalPlanta
Volume193
Issue number2
DOIs
Publication statusPublished - 1994 Mar
Externally publishedYes

Fingerprint

Photosystem I Protein Complex
Cucumis sativus
Thylakoids
Photosystem II Protein Complex
photosystem I
photoinhibition
photosystem II
Temperature
Light
thylakoids
leaves
temperature
Atmosphere
cucumbers
Suspensions
Electrons
ferricyanides
absorbance
electrons

Keywords

  • Chilling stress
  • Cucumis (Cucurbitaceae, cucumber)
  • Photoinhibition
  • Photosynthesis
  • Photosystem I
  • Thylakoid

ASJC Scopus subject areas

  • Plant Science

Cite this

The site of photoinhibition in leaves of Cucumis sativus L. at low temperatures is photosystem I, not photosystem II. / Terashima, Ichiro; Funayama, Sachiko; Sonoike, Kintake.

In: Planta, Vol. 193, No. 2, 03.1994, p. 300-306.

Research output: Contribution to journalArticle

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title = "The site of photoinhibition in leaves of Cucumis sativus L. at low temperatures is photosystem I, not photosystem II",
abstract = "Maximum quantum yields (QY) of photosynthetic electron flows through PSI and PSII were separately assessed in thylakoid membranes isolated from leaves of Cucumis sativus L. (cucumber) that had been chilled in various ways. The QY(PSI) in the thylakoids prepared from the leaves treated at 4° C in moderate light at 220 μmol quanta·m-2·s-1 (400-700 nm) for 5 h, was about 20-30{\%} of that in the thylakoids prepared from untreated leaves, while QY(PSII) decreased, at most, by 20{\%} in response to the same treatment. The decrease in QY(PSI) was observed only when the leaves were chilled at temperatures below 10° C, while such a marked temperature dependency was not observed for the decrease in QY(PSII). In the chilling treatment at 4° C for 5 h, the quantum flux density that was required to induce 50{\%} loss of QY (PSI) was ca. 50 umol quanta·m-2·s-1. When the chilling treatment at 4° C in the light was conducted in an atmosphere of N2, photoinhibition of PSI was largely suppressed, while the damage to PSII was somewhat enhanced. The ferricyanide-oxidised minus ascorbate-reduced difference spectra and the light-induced absorbance changes at 700 nm obtained with the thylakoid suspension, indicated the loss of P700 to extents that corresponded to the decreases in QY(PSI). Accordingly, the decreases in QY(PSI) can largely be attributed to destruction of the PSI reaction centre itself. These results clearly show that, at least in cucumber, a typical chillingsensitive plant, PSI is much more susceptible to aerobic photoinhibition than PSII.",
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AB - Maximum quantum yields (QY) of photosynthetic electron flows through PSI and PSII were separately assessed in thylakoid membranes isolated from leaves of Cucumis sativus L. (cucumber) that had been chilled in various ways. The QY(PSI) in the thylakoids prepared from the leaves treated at 4° C in moderate light at 220 μmol quanta·m-2·s-1 (400-700 nm) for 5 h, was about 20-30% of that in the thylakoids prepared from untreated leaves, while QY(PSII) decreased, at most, by 20% in response to the same treatment. The decrease in QY(PSI) was observed only when the leaves were chilled at temperatures below 10° C, while such a marked temperature dependency was not observed for the decrease in QY(PSII). In the chilling treatment at 4° C for 5 h, the quantum flux density that was required to induce 50% loss of QY (PSI) was ca. 50 umol quanta·m-2·s-1. When the chilling treatment at 4° C in the light was conducted in an atmosphere of N2, photoinhibition of PSI was largely suppressed, while the damage to PSII was somewhat enhanced. The ferricyanide-oxidised minus ascorbate-reduced difference spectra and the light-induced absorbance changes at 700 nm obtained with the thylakoid suspension, indicated the loss of P700 to extents that corresponded to the decreases in QY(PSI). Accordingly, the decreases in QY(PSI) can largely be attributed to destruction of the PSI reaction centre itself. These results clearly show that, at least in cucumber, a typical chillingsensitive plant, PSI is much more susceptible to aerobic photoinhibition than PSII.

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