The structure of l-amino-acid ligase from Bacillus licheniformis

Michihiko Suzuki, Yuichi Takahashi, Atsushi Noguchi, Toshinobu Arai, Makoto Yagasaki, Kuniki Kino, Jun Ichi Saito

    Research output: Contribution to journalArticle

    8 Citations (Scopus)

    Abstract

    l-Amino-acid ligases (LALs) are enzymes which catalyze the formation of dipeptides by linking two l-amino acids. Although many dipeptides are known and expected to have medical and nutritional benefits, their practical use has been limited owing to their low availability and high expense. LALs are potentially desirable tools for the efficient production of dipeptides; however, the molecular basis of substrate recognition by LAL has not yet been sufficiently elucidated for the design of ideal LALs for the desired dipeptides. This report presents the crystal structure of the LAL BL00235 derived from Bacillus licheniformis NBRC 12200 determined at 1.9 Å resolution using the multi-wavelength anomalous dispersion method. The overall structure of BL00235 is fairly similar to that of YwfE, the only LAL with a known structure, but the structure around the catalytic site contains some significant differences. Detailed structural comparison of BL00235 with YwfE sheds some light on the molecular basis of the substrate specificities.

    Original languageEnglish
    Pages (from-to)1535-1540
    Number of pages6
    JournalActa Crystallographica Section D: Biological Crystallography
    Volume68
    Issue number11
    DOIs
    Publication statusPublished - 2012 Nov

    Fingerprint

    Ligases
    Dipeptides
    Amino Acids
    Substrate Specificity
    Bacillus licheniformis
    Catalytic Domain
    Enzymes

    Keywords

    • dipeptides
    • l-amino-acid ligases
    • substrate specificity

    ASJC Scopus subject areas

    • Structural Biology

    Cite this

    The structure of l-amino-acid ligase from Bacillus licheniformis. / Suzuki, Michihiko; Takahashi, Yuichi; Noguchi, Atsushi; Arai, Toshinobu; Yagasaki, Makoto; Kino, Kuniki; Saito, Jun Ichi.

    In: Acta Crystallographica Section D: Biological Crystallography, Vol. 68, No. 11, 11.2012, p. 1535-1540.

    Research output: Contribution to journalArticle

    Suzuki, Michihiko ; Takahashi, Yuichi ; Noguchi, Atsushi ; Arai, Toshinobu ; Yagasaki, Makoto ; Kino, Kuniki ; Saito, Jun Ichi. / The structure of l-amino-acid ligase from Bacillus licheniformis. In: Acta Crystallographica Section D: Biological Crystallography. 2012 ; Vol. 68, No. 11. pp. 1535-1540.
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    AU - Yagasaki, Makoto

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