TY - JOUR
T1 - The t-loop region of animal mitochondrial trna(agy) is a main recognition site for homologous seryl-tRNA synthetase
AU - Ueda, Takuya
AU - Yotsumoto, Yoshihisa
AU - Ikeda, Kenzi
AU - Watanabe, Kimitsuna
N1 - Funding Information:
We thank Dr. H. Himeno for purifying T7 RNA polymerase. This work was supported by a Grant-in-Aid for Scientific Research on Priority Areas from the Ministry of Education, Science and Culture, Japan.
PY - 1992/5/11
Y1 - 1992/5/11
N2 - Recognition sites of bovine mitochondrial serine tRNA specific for codons AGY [tRNASer(AGY)] by the cognate mitochondrial seryl-tRNA synthetase were studied using a range of tRNASer(AGY) variants which were obtained by the in vitro transcription of synthetic tRNA genes with T7 RNA polymerase. Base replacements in the anticodon and discriminator sites did not affect serine acceptance. However, deletion and/or replacement in the T-loop region completely deprived the variants of their charging activities. Point mutation experiments in this region also showed that the adenosine residue in the middle of the T-loop (position 58), which is involved in tertiary interaction between the T-loop and the truncated D-arm [de Bruijn and Klug, 1983] played a significant role in the recognition process by the synthetase.
AB - Recognition sites of bovine mitochondrial serine tRNA specific for codons AGY [tRNASer(AGY)] by the cognate mitochondrial seryl-tRNA synthetase were studied using a range of tRNASer(AGY) variants which were obtained by the in vitro transcription of synthetic tRNA genes with T7 RNA polymerase. Base replacements in the anticodon and discriminator sites did not affect serine acceptance. However, deletion and/or replacement in the T-loop region completely deprived the variants of their charging activities. Point mutation experiments in this region also showed that the adenosine residue in the middle of the T-loop (position 58), which is involved in tertiary interaction between the T-loop and the truncated D-arm [de Bruijn and Klug, 1983] played a significant role in the recognition process by the synthetase.
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U2 - 10.1093/nar/20.9.2217
DO - 10.1093/nar/20.9.2217
M3 - Article
C2 - 1375735
AN - SCOPUS:0026516522
SN - 0305-1048
VL - 20
SP - 2217
EP - 2222
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 9
ER -