The titin-telethonin complex is a directed, superstable molecular bond in the muscle Z-disk

Morten Bertz, Matthias Wilmanns, Matthias Rief

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Mechanical stability of bonds and protein interactions has recently become accessible through single molecule mechanical experiments. So far, mechanical information about molecular bond mechanics has been largely limited to a single direction of force application. However, mechanical force acts as a vector in space and hence mechanical stability should depend on the direction of force application. In skeletal muscle, the giant protein titin is anchored in the Z-disk by telethonin. Much of the structural integrity of the Z-disk hinges upon the titin-telethonin bond. In this paper we show that the complex between the muscle proteins titin and telethonin forms a highly directed molecular bond. It is designed to resist ultra-high forces if they are applied in the direction along which it is loaded under physiological conditions, while it breaks easily along other directions. Highly directed molecular bonds match in an ideal way the requirements of tissues subject to mechanical stress.

Original languageEnglish
Pages (from-to)13307-13310
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume106
Issue number32
DOIs
Publication statusPublished - 2009 Aug 11
Externally publishedYes

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Connectin
Muscles
Mechanical Stress
Muscle Proteins
Protein Stability
Mechanics
Skeletal Muscle
Direction compound
Proteins

Keywords

  • Atomic force microscopy
  • Force spectroscopy
  • Protein engineering
  • Protein folding

ASJC Scopus subject areas

  • General

Cite this

The titin-telethonin complex is a directed, superstable molecular bond in the muscle Z-disk. / Bertz, Morten; Wilmanns, Matthias; Rief, Matthias.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, No. 32, 11.08.2009, p. 13307-13310.

Research output: Contribution to journalArticle

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