The tomato mosaic tobamovirus movement protein interacts with a putative transcriptional coactivator KELP

Yasuhiko Matsushita, Masakazu Deguchi, Masaru Youda, Masamichi Nishiguchi, Hiroshi Nyunoya

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Viral movement through plasmodesmata in host plants likely depends on the interaction between virus-encoded movement protein (MP) and host proteins. In order to search for MP-interacting protein (MIP), we carried out far-western screening of a Brassica campestris cDNA library using a recombinant MP of tomato mosaic tobamovirus (ToMV) as a probe. One of the positive clones, designated MIP102, was found to be a putative orthologue for a transcriptional coactivator KELP of Arabidopsis thaliana. In vitro analysis with recombinant proteins revealed that ToMV MP could bind to KELP proteins that are derived from different plant species. At least 31 amino acids from the carboxyl-terminus of ToMV MP were dispensable for the interaction with KELP. Other MPs, derived from crucifer tobamovirus CTMV-W and cucumber mosaic cucumovirus, also exhibited comparable binding abilities. This suggests that these MPs could commonly interact with KELP, possibly to modulate the host gene expression.

Original languageEnglish
Pages (from-to)57-66
Number of pages10
JournalMolecules and Cells
Volume12
Issue number1
Publication statusPublished - 2001 Aug 31
Externally publishedYes

Fingerprint

Tobamovirus
Lycopersicon esculentum
Proteins
Recombinant Proteins
Plasmodesmata
Cucumovirus
Cucumis sativus
Brassica
Gene Library
Arabidopsis
Clone Cells
Viruses
Gene Expression
Amino Acids

Keywords

  • Arabidopsis thaliana
  • Brassica campestris
  • Far-Western blot
  • KELP
  • Movement protein
  • MP-interacting protein
  • Plant virus
  • TMV
  • Tomato mosaic virus
  • Transcriptional coactivator

ASJC Scopus subject areas

  • Medicine(all)
  • Molecular Biology
  • Cell Biology

Cite this

The tomato mosaic tobamovirus movement protein interacts with a putative transcriptional coactivator KELP. / Matsushita, Yasuhiko; Deguchi, Masakazu; Youda, Masaru; Nishiguchi, Masamichi; Nyunoya, Hiroshi.

In: Molecules and Cells, Vol. 12, No. 1, 31.08.2001, p. 57-66.

Research output: Contribution to journalArticle

Matsushita, Yasuhiko ; Deguchi, Masakazu ; Youda, Masaru ; Nishiguchi, Masamichi ; Nyunoya, Hiroshi. / The tomato mosaic tobamovirus movement protein interacts with a putative transcriptional coactivator KELP. In: Molecules and Cells. 2001 ; Vol. 12, No. 1. pp. 57-66.
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