The transfer of titanium dioxide nanoparticles from the host plant to butterfly larvae through a food chain

Miyoko Kubo-Irie, Masaaki Yokoyama, Yusuke Shinkai, Rikio Niki, Ken Takeda, Masaru Irie

    Research output: Contribution to journalArticle

    9 Citations (Scopus)

    Abstract

    This study aimed to examine the transfer of nanoparticles within a terrestrial food chain. Oviposited eggs of the swallowtail butterfly (Atrophaneura alcinous) were hatched on the leaves of the host plant (Aristolochia debilis), and the root stock and root hairs were submerged in a suspension of 10 μg/ml titanium dioxide nanoparticles (TiO2-NPs) in a 100 ml bottle. The presence of TiO2-NPs in the veins of the leaves was confirmed by X-ray analytical microscopy (X-ray AM). The hatched 1st instar larvae fed on the leaves to moult into 2nd instar larvae. Small agglomerates of TiO2-NPs less than 150 nm in diameter were identified in the vascular tissue of the exposed plant, the midgut and the excreta of the larvae by transmission electron microscopy. The image of Ti elemental mapping by X-ray AM was analysed with the quantitative spatial information mapping (QSIM) technique. The results demonstrated that TiO2-NPs were transferred from the plant to the larvae and they were disseminated throughout the environment via larval excreta.

    Original languageEnglish
    Article number23819
    JournalScientific Reports
    Volume6
    DOIs
    Publication statusPublished - 2016 Mar 31

    Fingerprint

    Butterflies
    Food Chain
    Nanoparticles
    Larva
    Microscopy
    Aristolochia
    X-Rays
    Plant Leaves
    Transmission Electron Microscopy
    Eggs
    Blood Vessels
    Veins
    Suspensions
    titanium dioxide

    ASJC Scopus subject areas

    • General

    Cite this

    The transfer of titanium dioxide nanoparticles from the host plant to butterfly larvae through a food chain. / Kubo-Irie, Miyoko; Yokoyama, Masaaki; Shinkai, Yusuke; Niki, Rikio; Takeda, Ken; Irie, Masaru.

    In: Scientific Reports, Vol. 6, 23819, 31.03.2016.

    Research output: Contribution to journalArticle

    Kubo-Irie, Miyoko ; Yokoyama, Masaaki ; Shinkai, Yusuke ; Niki, Rikio ; Takeda, Ken ; Irie, Masaru. / The transfer of titanium dioxide nanoparticles from the host plant to butterfly larvae through a food chain. In: Scientific Reports. 2016 ; Vol. 6.
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    AU - Takeda, Ken

    AU - Irie, Masaru

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