The UBXN-2/p37/p47 adaptors of CDC-48/p97 regulate mitosis by limiting the centrosomal recruitment of Aurora A

Elsa Kress, Françoise Schwager, René Holtackers, Jonas Seiler, François Prodon, Esther Zanin, Annika Eiteneues, Mika Toya, Asako Sugimoto, Hemmo Meyer, Patrick Meraladi, Monica Gotta

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Coordination of cell cycle events in space and time is crucial to achieve a successful cell division. Here, we demonstrate that UBXN-2, a substrate adaptor of the AAA ATPase Cdc48/p97, is required to coordinate centrosome maturation timing with mitosis. In UBXN-2-depleted Caenorhabditis elegans embryos, centrosomes recruited more AIR-1 (Aurora A), matured precociously, and alignment of the mitotic spindle with the axis of polarity was impaired. UBXN-2 and CDC-48 coimmunoprecipitated with AIR-1 and the spindle alignment defect was partially rescued by co-depleting AIR-1, indicating that UBXN-2 controls these processes via AIR-1. Similarly, depletion in human cells of the UBXN-2 orthologues p37/p47 resulted in an accumulation of Aurora A at centrosomes and a delay in centrosome separation. The latter defect was also rescued by inhibiting Aurora A. We therefore postulate that the role of this adaptor in cell cycle regulation is conserved.

Original languageEnglish
Pages (from-to)559-575
Number of pages17
JournalJournal of Cell Biology
Volume201
Issue number4
DOIs
Publication statusPublished - 2013 May
Externally publishedYes

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Centrosome
Centers for Disease Control and Prevention (U.S.)
Mitosis
Cell Cycle
Spindle Apparatus
Caenorhabditis elegans
Cell Division
Embryonic Structures

ASJC Scopus subject areas

  • Cell Biology

Cite this

Kress, E., Schwager, F., Holtackers, R., Seiler, J., Prodon, F., Zanin, E., ... Gotta, M. (2013). The UBXN-2/p37/p47 adaptors of CDC-48/p97 regulate mitosis by limiting the centrosomal recruitment of Aurora A. Journal of Cell Biology, 201(4), 559-575. https://doi.org/10.1083/jcb.201209107

The UBXN-2/p37/p47 adaptors of CDC-48/p97 regulate mitosis by limiting the centrosomal recruitment of Aurora A. / Kress, Elsa; Schwager, Françoise; Holtackers, René; Seiler, Jonas; Prodon, François; Zanin, Esther; Eiteneues, Annika; Toya, Mika; Sugimoto, Asako; Meyer, Hemmo; Meraladi, Patrick; Gotta, Monica.

In: Journal of Cell Biology, Vol. 201, No. 4, 05.2013, p. 559-575.

Research output: Contribution to journalArticle

Kress, E, Schwager, F, Holtackers, R, Seiler, J, Prodon, F, Zanin, E, Eiteneues, A, Toya, M, Sugimoto, A, Meyer, H, Meraladi, P & Gotta, M 2013, 'The UBXN-2/p37/p47 adaptors of CDC-48/p97 regulate mitosis by limiting the centrosomal recruitment of Aurora A', Journal of Cell Biology, vol. 201, no. 4, pp. 559-575. https://doi.org/10.1083/jcb.201209107
Kress, Elsa ; Schwager, Françoise ; Holtackers, René ; Seiler, Jonas ; Prodon, François ; Zanin, Esther ; Eiteneues, Annika ; Toya, Mika ; Sugimoto, Asako ; Meyer, Hemmo ; Meraladi, Patrick ; Gotta, Monica. / The UBXN-2/p37/p47 adaptors of CDC-48/p97 regulate mitosis by limiting the centrosomal recruitment of Aurora A. In: Journal of Cell Biology. 2013 ; Vol. 201, No. 4. pp. 559-575.
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