The yeast linear DNA killer plasmids, pGKL1 and pGKL2, possess terminally attached proteins

Yo Kikuchi, Keiko Hirai, Fumio Hishinuma

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The terminal structures of linear DNA killer plasmids from yeast, pGKL1 and pGKL2, were analyzed. Results obtained by exonuclease treatments of these plasmids show that both pGKL plasmids have free hydroxyl 3′-ends and blocked 5′-ends. Electrophoretic analysis of the terminal restriction fragments treated with proteases revealed that pGKL1 and pGKL2 have proteins bound at 5′termini and that the terminal protein of pGKL1 is distinct from that of pGKL2. This is the first linear DNA-terminal protein association found in yeast.

Original languageEnglish
Pages (from-to)5685-5692
Number of pages8
JournalNucleic Acids Research
Volume12
Issue number14
DOIs
Publication statusPublished - 1984 Jul 25
Externally publishedYes

Fingerprint

Yeast
Plasmids
DNA
Yeasts
Proteins
Protein
Exonucleases
Protease
Hydroxyl Radical
Fragment
Peptide Hydrolases
Restriction
Distinct

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

The yeast linear DNA killer plasmids, pGKL1 and pGKL2, possess terminally attached proteins. / Kikuchi, Yo; Hirai, Keiko; Hishinuma, Fumio.

In: Nucleic Acids Research, Vol. 12, No. 14, 25.07.1984, p. 5685-5692.

Research output: Contribution to journalArticle

Kikuchi, Yo ; Hirai, Keiko ; Hishinuma, Fumio. / The yeast linear DNA killer plasmids, pGKL1 and pGKL2, possess terminally attached proteins. In: Nucleic Acids Research. 1984 ; Vol. 12, No. 14. pp. 5685-5692.
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