The yeast linear DNA killer plasmids, pGKL1 and pGKL2, possess terminally attached proteins

Yo Kikuchi, Keiko Hirai, Fumio Hishinuma

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The terminal structures of linear DNA killer plasmids from yeast, pGKL1 and pGKL2, were analyzed. Results obtained by exonuclease treatments of these plasmids show that both pGKL plasmids have free hydroxyl 3′-ends and blocked 5′-ends. Electrophoretic analysis of the terminal restriction fragments treated with proteases revealed that pGKL1 and pGKL2 have proteins bound at 5′termini and that the terminal protein of pGKL1 is distinct from that of pGKL2. This is the first linear DNA-terminal protein association found in yeast.

Original languageEnglish
Pages (from-to)5685-5692
Number of pages8
JournalNucleic Acids Research
Volume12
Issue number14
DOIs
Publication statusPublished - 1984 Jul 25
Externally publishedYes

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Fingerprint Dive into the research topics of 'The yeast linear DNA killer plasmids, pGKL1 and pGKL2, possess terminally attached proteins'. Together they form a unique fingerprint.

  • Cite this