Theoretical optimization method of buffer ionic concentration for protein detection using field effect transistors

Sho Hideshima, Hila Einati, Takahiro Nakamura, Shigeki Kuroiwa, Yosi Shacham-Diamand, Tetsuya Osaka

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Field effect transistors (FETs) may respond to charge variations occurring when proteins are adsorbed to the gate surface. The electrolyte electrostatic screening allows a practical detection of charges that are only within a distance of approximately the double layer thickness, which is related to the Debye length in the electrolyte. Hence, operating the FET biomolecular sensors at the highest possible ionic concentration improves reproducibility; however, it may reduce the signal if the concentration is too high. Here, we propose an optimization method of the buffer ionic concentration using a figure of merit "charge number," Zd, which is defined as the ratio between the effective number of the surface charges and the number of immobilized molecules. The theoretical Zd was calculated using a three-dimensional conformation data taken from the Protein Data Bank. The Z d was obtained from the total number of four charged amino acids of avidin, arginine (positive), lysine (positive), aspartic acid (negative), and glutamic acid (negative), which are within the double layer length from the surface. To verify this model, we chose avidin-biotin interaction. The experimental Zd, which was obtained for three buffer concentrations, matched the theoretical Zd. This method shortens the solution calibration time and reduces the analyte amount.

Original languageEnglish
JournalJournal of the Electrochemical Society
Volume157
Issue number12
DOIs
Publication statusPublished - 2010

Fingerprint

Avidin
Field effect transistors
Electrolytes
Buffers
field effect transistors
buffers
proteins
Proteins
Arginine
optimization
Acids
Surface charge
Biotin
Aspartic Acid
Lysine
Conformations
Amino acids
Glutamic Acid
Electrostatics
Screening

ASJC Scopus subject areas

  • Electrochemistry
  • Electronic, Optical and Magnetic Materials
  • Materials Chemistry
  • Surfaces, Coatings and Films
  • Renewable Energy, Sustainability and the Environment
  • Condensed Matter Physics

Cite this

Theoretical optimization method of buffer ionic concentration for protein detection using field effect transistors. / Hideshima, Sho; Einati, Hila; Nakamura, Takahiro; Kuroiwa, Shigeki; Shacham-Diamand, Yosi; Osaka, Tetsuya.

In: Journal of the Electrochemical Society, Vol. 157, No. 12, 2010.

Research output: Contribution to journalArticle

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AU - Osaka, Tetsuya

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