Therapeutic efficacy of an antibiotic-loaded nanosheet in a murine burn-wound infection model

Akihiro Saito, Hiromi Miyazaki, Toshinori Fujie, Shinya Ohtsubo, Manabu Kinoshita, Daizoh Saitoh, Shinji Takeoka

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Polymeric ultra-thin films (nanosheets) possess unique properties that make them suitable materials for various biomedical applications. In our previous study, we assessed the use of an antibiotic (tetracycline, TC)-loaded nanosheet (or "TC-nanosheet") for the treatment of gastrointestinal tissue defects. The nanosheet consisted of three functional layers: layer-by-layer nanosheet as a stable platform, TC as an antimicrobial agent with autofluorescence for tracing, and a poly(vinyl acetate) nanosheet to act as a protecting layer. The TC-nanosheet has high flexibility, adhesive strength and transparency. Here, we evaluated the effectiveness of the TC-nanosheet in preventing full thickness burn-wound infections. In an in vivo study, murine dorsal skin was injured by full-thickness burns and then infected with Pseudomonas aeruginosa (P. aeruginosa), a common bacterium causing burn-associated infections. The wound site was treated either with a TC-nanosheet, TC-unloaded nanosheet or left untreated. Wound management was facilitated by the high transparency of the TC-nanosheet. The TC-nanosheet significantly improved burn-wound infection by P. aeruginosa in mice. Indeed, all mice treated with the TC-nanosheet survived, whereas the other treatment groups displayed increased rates of mortality due to bacterial infection. According to histological analyses and viable bacterial counting in the liver (bacterial translocation), the TC-nanosheets were able to prevent not only the local inflammation but also systemic inflammation. We conclude that the TC-nanosheet can act as an effective treatment for full-thickness burn-wound infection. Hence, the TC-nanosheet is a promising therapeutic tool for burn-wound management in severely burn-injured patients.

Original languageEnglish
Pages (from-to)2932-2940
Number of pages9
JournalActa Biomaterialia
Volume8
Issue number8
DOIs
Publication statusPublished - 2012 Aug

Fingerprint

Nanosheets
Antibiotics
Wound Infection
Tetracycline
Burns
Anti-Bacterial Agents
Therapeutics
Pseudomonas aeruginosa
Wounds and Injuries
Transparency
Inflammation
Bacterial Translocation
Antimicrobial agents
Anti-Infective Agents
Bacterial Infections
Adhesives
Ultrathin films
Polymer films
Liver

Keywords

  • Antibiotic
  • Full-thickness burn-wound infection
  • Polymer nanosheet
  • Wound protective material

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Biotechnology
  • Biochemistry
  • Molecular Biology

Cite this

Therapeutic efficacy of an antibiotic-loaded nanosheet in a murine burn-wound infection model. / Saito, Akihiro; Miyazaki, Hiromi; Fujie, Toshinori; Ohtsubo, Shinya; Kinoshita, Manabu; Saitoh, Daizoh; Takeoka, Shinji.

In: Acta Biomaterialia, Vol. 8, No. 8, 08.2012, p. 2932-2940.

Research output: Contribution to journalArticle

Saito, Akihiro ; Miyazaki, Hiromi ; Fujie, Toshinori ; Ohtsubo, Shinya ; Kinoshita, Manabu ; Saitoh, Daizoh ; Takeoka, Shinji. / Therapeutic efficacy of an antibiotic-loaded nanosheet in a murine burn-wound infection model. In: Acta Biomaterialia. 2012 ; Vol. 8, No. 8. pp. 2932-2940.
@article{50707842053146f2a29fab111886e18f,
title = "Therapeutic efficacy of an antibiotic-loaded nanosheet in a murine burn-wound infection model",
abstract = "Polymeric ultra-thin films (nanosheets) possess unique properties that make them suitable materials for various biomedical applications. In our previous study, we assessed the use of an antibiotic (tetracycline, TC)-loaded nanosheet (or {"}TC-nanosheet{"}) for the treatment of gastrointestinal tissue defects. The nanosheet consisted of three functional layers: layer-by-layer nanosheet as a stable platform, TC as an antimicrobial agent with autofluorescence for tracing, and a poly(vinyl acetate) nanosheet to act as a protecting layer. The TC-nanosheet has high flexibility, adhesive strength and transparency. Here, we evaluated the effectiveness of the TC-nanosheet in preventing full thickness burn-wound infections. In an in vivo study, murine dorsal skin was injured by full-thickness burns and then infected with Pseudomonas aeruginosa (P. aeruginosa), a common bacterium causing burn-associated infections. The wound site was treated either with a TC-nanosheet, TC-unloaded nanosheet or left untreated. Wound management was facilitated by the high transparency of the TC-nanosheet. The TC-nanosheet significantly improved burn-wound infection by P. aeruginosa in mice. Indeed, all mice treated with the TC-nanosheet survived, whereas the other treatment groups displayed increased rates of mortality due to bacterial infection. According to histological analyses and viable bacterial counting in the liver (bacterial translocation), the TC-nanosheets were able to prevent not only the local inflammation but also systemic inflammation. We conclude that the TC-nanosheet can act as an effective treatment for full-thickness burn-wound infection. Hence, the TC-nanosheet is a promising therapeutic tool for burn-wound management in severely burn-injured patients.",
keywords = "Antibiotic, Full-thickness burn-wound infection, Polymer nanosheet, Wound protective material",
author = "Akihiro Saito and Hiromi Miyazaki and Toshinori Fujie and Shinya Ohtsubo and Manabu Kinoshita and Daizoh Saitoh and Shinji Takeoka",
year = "2012",
month = "8",
doi = "10.1016/j.actbio.2012.04.019",
language = "English",
volume = "8",
pages = "2932--2940",
journal = "Acta Biomaterialia",
issn = "1742-7061",
publisher = "Elsevier BV",
number = "8",

}

TY - JOUR

T1 - Therapeutic efficacy of an antibiotic-loaded nanosheet in a murine burn-wound infection model

AU - Saito, Akihiro

AU - Miyazaki, Hiromi

AU - Fujie, Toshinori

AU - Ohtsubo, Shinya

AU - Kinoshita, Manabu

AU - Saitoh, Daizoh

AU - Takeoka, Shinji

PY - 2012/8

Y1 - 2012/8

N2 - Polymeric ultra-thin films (nanosheets) possess unique properties that make them suitable materials for various biomedical applications. In our previous study, we assessed the use of an antibiotic (tetracycline, TC)-loaded nanosheet (or "TC-nanosheet") for the treatment of gastrointestinal tissue defects. The nanosheet consisted of three functional layers: layer-by-layer nanosheet as a stable platform, TC as an antimicrobial agent with autofluorescence for tracing, and a poly(vinyl acetate) nanosheet to act as a protecting layer. The TC-nanosheet has high flexibility, adhesive strength and transparency. Here, we evaluated the effectiveness of the TC-nanosheet in preventing full thickness burn-wound infections. In an in vivo study, murine dorsal skin was injured by full-thickness burns and then infected with Pseudomonas aeruginosa (P. aeruginosa), a common bacterium causing burn-associated infections. The wound site was treated either with a TC-nanosheet, TC-unloaded nanosheet or left untreated. Wound management was facilitated by the high transparency of the TC-nanosheet. The TC-nanosheet significantly improved burn-wound infection by P. aeruginosa in mice. Indeed, all mice treated with the TC-nanosheet survived, whereas the other treatment groups displayed increased rates of mortality due to bacterial infection. According to histological analyses and viable bacterial counting in the liver (bacterial translocation), the TC-nanosheets were able to prevent not only the local inflammation but also systemic inflammation. We conclude that the TC-nanosheet can act as an effective treatment for full-thickness burn-wound infection. Hence, the TC-nanosheet is a promising therapeutic tool for burn-wound management in severely burn-injured patients.

AB - Polymeric ultra-thin films (nanosheets) possess unique properties that make them suitable materials for various biomedical applications. In our previous study, we assessed the use of an antibiotic (tetracycline, TC)-loaded nanosheet (or "TC-nanosheet") for the treatment of gastrointestinal tissue defects. The nanosheet consisted of three functional layers: layer-by-layer nanosheet as a stable platform, TC as an antimicrobial agent with autofluorescence for tracing, and a poly(vinyl acetate) nanosheet to act as a protecting layer. The TC-nanosheet has high flexibility, adhesive strength and transparency. Here, we evaluated the effectiveness of the TC-nanosheet in preventing full thickness burn-wound infections. In an in vivo study, murine dorsal skin was injured by full-thickness burns and then infected with Pseudomonas aeruginosa (P. aeruginosa), a common bacterium causing burn-associated infections. The wound site was treated either with a TC-nanosheet, TC-unloaded nanosheet or left untreated. Wound management was facilitated by the high transparency of the TC-nanosheet. The TC-nanosheet significantly improved burn-wound infection by P. aeruginosa in mice. Indeed, all mice treated with the TC-nanosheet survived, whereas the other treatment groups displayed increased rates of mortality due to bacterial infection. According to histological analyses and viable bacterial counting in the liver (bacterial translocation), the TC-nanosheets were able to prevent not only the local inflammation but also systemic inflammation. We conclude that the TC-nanosheet can act as an effective treatment for full-thickness burn-wound infection. Hence, the TC-nanosheet is a promising therapeutic tool for burn-wound management in severely burn-injured patients.

KW - Antibiotic

KW - Full-thickness burn-wound infection

KW - Polymer nanosheet

KW - Wound protective material

UR - http://www.scopus.com/inward/record.url?scp=84863188091&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84863188091&partnerID=8YFLogxK

U2 - 10.1016/j.actbio.2012.04.019

DO - 10.1016/j.actbio.2012.04.019

M3 - Article

VL - 8

SP - 2932

EP - 2940

JO - Acta Biomaterialia

JF - Acta Biomaterialia

SN - 1742-7061

IS - 8

ER -