Thermal imaging of receptor-activated heart production in single cells

Ofer Zohar, Masayaki Ikeda, Hiroyuki Shinagawa, Hiroko Inoue, Hiroshi Nakamura, Danek Elbaum, Daniel L. Alkon, Tohru Yoshioka

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    Abstract

    Changes in enthalpy (i.e., heat content) occur during the diverse intracellular chemical and biophysical interactions that take place in the life cycle of biological cells. Such changes have previously been measured for cell suspensions or cell-free biochemical extracts by using microcalorimetry, thermocouples, or pyroelectric films, all of which afford minimal spatial or temporal resolution. Here we present a novel thermal imaging method that combines method diffraction-limited spatial (~300 nm) and sampling-rate-limited time resolution, using the temperature-dependent phosphorescence intensity of the rare earth chelate Eu-TTA (europium (III) thenoyltrifluoro-acetonate). With this thermosensitive dye, we imaged intracellular heat waves evoked in Chinese hamster ovary cells after activation of the metabotropic m1-muscarinic receptor. Fast application of acetylcholine onto the cells evoked a biphasic heat wave that was blocked by atropine, and after a brief delay was followed by a calcium wave. Atropine applied by itself produced a monophasic heat wave in the cells, suggesting that its interactions with the receptor activate some intracellular metabolic pathways. The thermal imaging technique introduced here should provide new insights into cellular functions by resolving the location, kinetics, and quantity of intracellular heat production.

    Original languageEnglish
    Pages (from-to)82-89
    Number of pages8
    JournalBiophysical Journal
    Volume74
    Issue number1
    Publication statusPublished - 1998 Jan

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    ASJC Scopus subject areas

    • Biophysics

    Cite this

    Zohar, O., Ikeda, M., Shinagawa, H., Inoue, H., Nakamura, H., Elbaum, D., Alkon, D. L., & Yoshioka, T. (1998). Thermal imaging of receptor-activated heart production in single cells. Biophysical Journal, 74(1), 82-89.