Thermophilic biodesulfurization of hydrodesulfurized light gas oils by Mycobacterium phlei WU-F1

Toshiki Furuya, Yoshitaka Ishii, Ken ichi Noda, Kuniki Kino, Kotaro Kirimura

    Research output: Contribution to journalArticle

    48 Citations (Scopus)

    Abstract

    Recalcitrant organosulfur compounds such as dibenzothiophene (DBT) derivatives in light gas oil (LGO) cannot be removed by conventional hydrodesulfurization (HDS) treatment using metallic catalysts. The thermophilic DBT-desulfurizing bacterium Mycobacterium phlei WU-F1 grew in a medium with hydrodesulfurized LGO as the sole source of sulfur, and exhibited high desulfurizing ability toward LGO between 30 and 50°C. When WU-F1 was cultivated at 45°C with B-LGO (390 ppm S), F-LGO (120 ppm S) or X-LGO (34 ppm S) as the sole source of sulfur, biodesulfurization resulted in around 60-70% reduction of sulfur content for all three types of hydrodesulfurized LGOs. In addition, when resting cells were incubated at 45°C with hydrodesulfurized LGOs in the reaction mixtures containing 50% (v/v) oils, biodesulfurization reduced the sulfur content from 390 to 100 ppm S (B-LGO), from 120 to 42 ppm S (F-LGO) and from 34 to 15 ppm S (X-LGO). Gas chromatography analysis with an atomic emission detector revealed that the peaks of alkylated DBTs including 4-methyl-DBT, 4,6-dimethyl-DBT and 3,4,6-trimethyl-DBT significantly decreased after biodesulfurization. Therefore, thermophilic M. phlei WU-F1, which could effectively desulfurize HDS-treated LGOs over a wide temperature range up to 50°C, may be a promising biocatalyst for practical biodesulfurization of diesel oil.

    Original languageEnglish
    Pages (from-to)137-142
    Number of pages6
    JournalFEMS Microbiology Letters
    Volume221
    Issue number1
    DOIs
    Publication statusPublished - 2003 Apr 11

    Fingerprint

    Mycobacterium phlei
    Oils
    Gases
    Sulfur
    Gas Chromatography

    Keywords

    • Desulfurization
    • Dibenzothiophene
    • Diesel oil
    • Light gas oil
    • Mycobacterium phlei

    ASJC Scopus subject areas

    • Genetics
    • Molecular Biology
    • Applied Microbiology and Biotechnology
    • Microbiology

    Cite this

    Thermophilic biodesulfurization of hydrodesulfurized light gas oils by Mycobacterium phlei WU-F1. / Furuya, Toshiki; Ishii, Yoshitaka; Noda, Ken ichi; Kino, Kuniki; Kirimura, Kotaro.

    In: FEMS Microbiology Letters, Vol. 221, No. 1, 11.04.2003, p. 137-142.

    Research output: Contribution to journalArticle

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    abstract = "Recalcitrant organosulfur compounds such as dibenzothiophene (DBT) derivatives in light gas oil (LGO) cannot be removed by conventional hydrodesulfurization (HDS) treatment using metallic catalysts. The thermophilic DBT-desulfurizing bacterium Mycobacterium phlei WU-F1 grew in a medium with hydrodesulfurized LGO as the sole source of sulfur, and exhibited high desulfurizing ability toward LGO between 30 and 50°C. When WU-F1 was cultivated at 45°C with B-LGO (390 ppm S), F-LGO (120 ppm S) or X-LGO (34 ppm S) as the sole source of sulfur, biodesulfurization resulted in around 60-70{\%} reduction of sulfur content for all three types of hydrodesulfurized LGOs. In addition, when resting cells were incubated at 45°C with hydrodesulfurized LGOs in the reaction mixtures containing 50{\%} (v/v) oils, biodesulfurization reduced the sulfur content from 390 to 100 ppm S (B-LGO), from 120 to 42 ppm S (F-LGO) and from 34 to 15 ppm S (X-LGO). Gas chromatography analysis with an atomic emission detector revealed that the peaks of alkylated DBTs including 4-methyl-DBT, 4,6-dimethyl-DBT and 3,4,6-trimethyl-DBT significantly decreased after biodesulfurization. Therefore, thermophilic M. phlei WU-F1, which could effectively desulfurize HDS-treated LGOs over a wide temperature range up to 50°C, may be a promising biocatalyst for practical biodesulfurization of diesel oil.",
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