Thermoresponsive cationic copolymer brushes for mesenchymal stem cell separation

Kenichi Nagase, Yuri Hatakeyama, Tatsuya Shimizu, Katsuhisa Matsuura, Masayuki Yamato, Naoya Takeda, Teruo Okano

    Research output: Contribution to journalArticle

    30 Citations (Scopus)

    Abstract

    Thermoresponsive, cationic, copolymer brushes poly(N-isopropylacrylamide(IPAAm)-co-N,N-dimethylaminopropylacrylamide-co-N-tert-butylacrylamide(tBAAm)) and poly(IPAAm-co-3-acrylamidopropyl trimethylammonium chloride-co-tBAAm) were prepared on glass substrates through surface-initiated atom transfer radical polymerization. Prepared copolymer brushes were investigated as thermally modulated cell separation materials. Densely packed cationic copolymer brushes were formed on the glass substrates, and the positive charge density was modulated by controlling the composition of cationic moieties and species. During observation of cell adhesion and detachment properties on copolymer brushes, human bone marrow mesenchymal stem cells (hbmMSC) exhibited thermally modulated cell adhesion and detachment, while other bone-marrow-derived cells did not adhere. Using these properties, hbmMSC could be purified from mixtures of human bone-marrow-derived cells simply by changing the external temperature. Therefore, the prepared cationic copolymer brush is useful for separation of hbmMSC.

    Original languageEnglish
    Pages (from-to)532-540
    Number of pages9
    JournalBiomacromolecules
    Volume16
    Issue number2
    DOIs
    Publication statusPublished - 2015 Feb 9

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    Brushes
    Stem cells
    Bone
    Copolymers
    Cell adhesion
    Glass
    Atom transfer radical polymerization
    Substrates
    Charge density
    Chemical analysis
    Temperature
    poly-N-isopropylacrylamide

    ASJC Scopus subject areas

    • Bioengineering
    • Materials Chemistry
    • Polymers and Plastics
    • Biomaterials

    Cite this

    Nagase, K., Hatakeyama, Y., Shimizu, T., Matsuura, K., Yamato, M., Takeda, N., & Okano, T. (2015). Thermoresponsive cationic copolymer brushes for mesenchymal stem cell separation. Biomacromolecules, 16(2), 532-540. https://doi.org/10.1021/bm501591s

    Thermoresponsive cationic copolymer brushes for mesenchymal stem cell separation. / Nagase, Kenichi; Hatakeyama, Yuri; Shimizu, Tatsuya; Matsuura, Katsuhisa; Yamato, Masayuki; Takeda, Naoya; Okano, Teruo.

    In: Biomacromolecules, Vol. 16, No. 2, 09.02.2015, p. 532-540.

    Research output: Contribution to journalArticle

    Nagase, K, Hatakeyama, Y, Shimizu, T, Matsuura, K, Yamato, M, Takeda, N & Okano, T 2015, 'Thermoresponsive cationic copolymer brushes for mesenchymal stem cell separation', Biomacromolecules, vol. 16, no. 2, pp. 532-540. https://doi.org/10.1021/bm501591s
    Nagase, Kenichi ; Hatakeyama, Yuri ; Shimizu, Tatsuya ; Matsuura, Katsuhisa ; Yamato, Masayuki ; Takeda, Naoya ; Okano, Teruo. / Thermoresponsive cationic copolymer brushes for mesenchymal stem cell separation. In: Biomacromolecules. 2015 ; Vol. 16, No. 2. pp. 532-540.
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    AU - Shimizu, Tatsuya

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    AU - Yamato, Masayuki

    AU - Takeda, Naoya

    AU - Okano, Teruo

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