Thermoresponsive polymer-modified microfibers for cell separations

Kenichi Nagase, Yoichi Sakurada, Satoru Onizuka, Takanori Iwata, Masayuki Yamato, Naoya Takeda, Teruo Okano

    Research output: Contribution to journalArticle

    10 Citations (Scopus)

    Abstract

    Thermoresponsive polymer-modified microfibers were prepared through electrospinning of poly(4-vinylbenzyl chloride) (PVBC) and subsequent surface-initiated atom transfer radical polymerization for grafting poly(N-isopropylacrylamide) (PIPAAm). Electrospinning conditions were optimized to produce large-diameter (20. μm) PVBC microfibers. The amount of PIPAAm grafted on the microfibers was controlled via the IPAAm monomer concentration. The microfibers exhibited thermally controlled cell separation by selective adhesion of normal human dermal fibroblasts in a mixed cell suspension that also contained human umbilical vein endothelial cells. In addition, adipose-derived stem cells (ADSCs) exhibited thermally modulated cell adhesion and detachment, while adhesion of other ADSC-related cells was low. Thus, ADSCs could be separated from a mixture of adipose tissue-derived cells simply by changing the temperature. Overall, the PIPAAm-modified microfibers are potentially applicable as temperature-modulated cell separation materials. Statement of Significance: Thermoresponsive poly(N-isopropylacrylamide) (PIPAAm) polymer-modified poly(4-vinylbenzyl chloride) (PVBC) microfibers were prepared via electrospinning of PVBC, followed by surface-initiated ATRP. They formed effective thermally-modulated cell separation materials with large surface areas. Cells adhered and extended along the modified microfibers; this was not observed on previously reported PIPAAm-modified flat substrates. The cellular adhesion enabled separation of fibroblast cells, as well as that of adipose-derived mesenchymal stem cells, from mixtures of similar cells. Thus, the temperature-controlled thermoresponsive microfibers would be potentially useful as cell separation materials.

    Original languageEnglish
    JournalActa Biomaterialia
    DOIs
    Publication statusAccepted/In press - 2016 Aug 5

    Fingerprint

    Cell Separation
    Polymers
    Stem cells
    Chlorides
    Electrospinning
    Stem Cells
    Adhesion
    Atom transfer radical polymerization
    Fibroblasts
    Temperature
    Cell adhesion
    Endothelial cells
    Human Umbilical Vein Endothelial Cells
    Mesenchymal Stromal Cells
    Cell Adhesion
    Polymerization
    Adipose Tissue
    Suspensions
    Monomers
    Cells

    Keywords

    • Cell separation
    • Electrospun microfiber
    • Polymer brush
    • Regenerative medicine
    • Stimuli responsive polymer

    ASJC Scopus subject areas

    • Biotechnology
    • Biochemistry
    • Biomaterials
    • Biomedical Engineering
    • Molecular Biology

    Cite this

    Nagase, K., Sakurada, Y., Onizuka, S., Iwata, T., Yamato, M., Takeda, N., & Okano, T. (Accepted/In press). Thermoresponsive polymer-modified microfibers for cell separations. Acta Biomaterialia. https://doi.org/10.1016/j.actbio.2017.02.033

    Thermoresponsive polymer-modified microfibers for cell separations. / Nagase, Kenichi; Sakurada, Yoichi; Onizuka, Satoru; Iwata, Takanori; Yamato, Masayuki; Takeda, Naoya; Okano, Teruo.

    In: Acta Biomaterialia, 05.08.2016.

    Research output: Contribution to journalArticle

    Nagase, Kenichi ; Sakurada, Yoichi ; Onizuka, Satoru ; Iwata, Takanori ; Yamato, Masayuki ; Takeda, Naoya ; Okano, Teruo. / Thermoresponsive polymer-modified microfibers for cell separations. In: Acta Biomaterialia. 2016.
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    AU - Nagase, Kenichi

    AU - Sakurada, Yoichi

    AU - Onizuka, Satoru

    AU - Iwata, Takanori

    AU - Yamato, Masayuki

    AU - Takeda, Naoya

    AU - Okano, Teruo

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    AB - Thermoresponsive polymer-modified microfibers were prepared through electrospinning of poly(4-vinylbenzyl chloride) (PVBC) and subsequent surface-initiated atom transfer radical polymerization for grafting poly(N-isopropylacrylamide) (PIPAAm). Electrospinning conditions were optimized to produce large-diameter (20. μm) PVBC microfibers. The amount of PIPAAm grafted on the microfibers was controlled via the IPAAm monomer concentration. The microfibers exhibited thermally controlled cell separation by selective adhesion of normal human dermal fibroblasts in a mixed cell suspension that also contained human umbilical vein endothelial cells. In addition, adipose-derived stem cells (ADSCs) exhibited thermally modulated cell adhesion and detachment, while adhesion of other ADSC-related cells was low. Thus, ADSCs could be separated from a mixture of adipose tissue-derived cells simply by changing the temperature. Overall, the PIPAAm-modified microfibers are potentially applicable as temperature-modulated cell separation materials. Statement of Significance: Thermoresponsive poly(N-isopropylacrylamide) (PIPAAm) polymer-modified poly(4-vinylbenzyl chloride) (PVBC) microfibers were prepared via electrospinning of PVBC, followed by surface-initiated ATRP. They formed effective thermally-modulated cell separation materials with large surface areas. Cells adhered and extended along the modified microfibers; this was not observed on previously reported PIPAAm-modified flat substrates. The cellular adhesion enabled separation of fibroblast cells, as well as that of adipose-derived mesenchymal stem cells, from mixtures of similar cells. Thus, the temperature-controlled thermoresponsive microfibers would be potentially useful as cell separation materials.

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    KW - Electrospun microfiber

    KW - Polymer brush

    KW - Regenerative medicine

    KW - Stimuli responsive polymer

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