Three-dimensional sheath flow sorting microsystem using thermosensitive hydrogel

Takahiro Arakawa, Yoshitaka Shirasaki, Tokihiko Aoki, Takashi Funatsu, Shuichi Shoji

    Research output: Contribution to journalArticle

    29 Citations (Scopus)

    Abstract

    This paper details a three-dimensional sheath flow sorting microsystem for separation and collection of biomolecules. High-speed particle and biomolecule 3D sheath flow sorting system was realized using reversible thermosensitive hydrogel. The sol-gel transfer of the thermosensitive hydrogel generated by focused IR laser irradiation performs flow switching, the response time of the sol-gel transformation using Mebiol Gel™ was less than 1 ms. For actual bio-applications, accurate and high-speed micro sorter is realized by optimizing the channel structure and by utilizing 3D sheath flow. High-speed sorting less than 3.0 ms from detection to separation of targets and no error sorting for 45 min (100,000 counts) in 3D sheath flow sorting system was realized. 3D sheath flow can be focused to 1.2 μm of transverse distribution and 6.0 μm of longitudinal distribution in regard to microbeads of sample flow. The same flow velocity and flow line of the focused sample flow are kept at constant laser irradiation time and power to sort the target. Since sorting was performed in simple and disposable PDMS-glass microchannels without any electric stimulation and mechanical valve structures, the proposed system is suitable for many biochemical applications.

    Original languageEnglish
    Pages (from-to)99-105
    Number of pages7
    JournalSensors and Actuators, A: Physical
    Volume135
    Issue number1
    DOIs
    Publication statusPublished - 2007 Mar 30

    Fingerprint

    Hydrogel
    Microsystems
    classifying
    Sorting
    sheaths
    Hydrogels
    Biomolecules
    Laser beam effects
    Sol-gels
    high speed
    gels
    Microchannels
    Flow velocity
    irradiation
    Gels
    microchannels
    stimulation
    Glass
    infrared lasers
    flow velocity

    Keywords

    • Particle and biomolecule sorter
    • PDMS microsystem
    • Thermosensitive hydrogel
    • Three-dimensional sheath flow

    ASJC Scopus subject areas

    • Electrical and Electronic Engineering
    • Mechanical Engineering
    • Instrumentation

    Cite this

    Three-dimensional sheath flow sorting microsystem using thermosensitive hydrogel. / Arakawa, Takahiro; Shirasaki, Yoshitaka; Aoki, Tokihiko; Funatsu, Takashi; Shoji, Shuichi.

    In: Sensors and Actuators, A: Physical, Vol. 135, No. 1, 30.03.2007, p. 99-105.

    Research output: Contribution to journalArticle

    Arakawa, Takahiro ; Shirasaki, Yoshitaka ; Aoki, Tokihiko ; Funatsu, Takashi ; Shoji, Shuichi. / Three-dimensional sheath flow sorting microsystem using thermosensitive hydrogel. In: Sensors and Actuators, A: Physical. 2007 ; Vol. 135, No. 1. pp. 99-105.
    @article{b83a31ef27c449b48ebb6de2c31b47c6,
    title = "Three-dimensional sheath flow sorting microsystem using thermosensitive hydrogel",
    abstract = "This paper details a three-dimensional sheath flow sorting microsystem for separation and collection of biomolecules. High-speed particle and biomolecule 3D sheath flow sorting system was realized using reversible thermosensitive hydrogel. The sol-gel transfer of the thermosensitive hydrogel generated by focused IR laser irradiation performs flow switching, the response time of the sol-gel transformation using Mebiol Gel™ was less than 1 ms. For actual bio-applications, accurate and high-speed micro sorter is realized by optimizing the channel structure and by utilizing 3D sheath flow. High-speed sorting less than 3.0 ms from detection to separation of targets and no error sorting for 45 min (100,000 counts) in 3D sheath flow sorting system was realized. 3D sheath flow can be focused to 1.2 μm of transverse distribution and 6.0 μm of longitudinal distribution in regard to microbeads of sample flow. The same flow velocity and flow line of the focused sample flow are kept at constant laser irradiation time and power to sort the target. Since sorting was performed in simple and disposable PDMS-glass microchannels without any electric stimulation and mechanical valve structures, the proposed system is suitable for many biochemical applications.",
    keywords = "Particle and biomolecule sorter, PDMS microsystem, Thermosensitive hydrogel, Three-dimensional sheath flow",
    author = "Takahiro Arakawa and Yoshitaka Shirasaki and Tokihiko Aoki and Takashi Funatsu and Shuichi Shoji",
    year = "2007",
    month = "3",
    day = "30",
    doi = "10.1016/j.sna.2006.06.074",
    language = "English",
    volume = "135",
    pages = "99--105",
    journal = "Sensors and Actuators, A: Physical",
    issn = "0924-4247",
    publisher = "Elsevier",
    number = "1",

    }

    TY - JOUR

    T1 - Three-dimensional sheath flow sorting microsystem using thermosensitive hydrogel

    AU - Arakawa, Takahiro

    AU - Shirasaki, Yoshitaka

    AU - Aoki, Tokihiko

    AU - Funatsu, Takashi

    AU - Shoji, Shuichi

    PY - 2007/3/30

    Y1 - 2007/3/30

    N2 - This paper details a three-dimensional sheath flow sorting microsystem for separation and collection of biomolecules. High-speed particle and biomolecule 3D sheath flow sorting system was realized using reversible thermosensitive hydrogel. The sol-gel transfer of the thermosensitive hydrogel generated by focused IR laser irradiation performs flow switching, the response time of the sol-gel transformation using Mebiol Gel™ was less than 1 ms. For actual bio-applications, accurate and high-speed micro sorter is realized by optimizing the channel structure and by utilizing 3D sheath flow. High-speed sorting less than 3.0 ms from detection to separation of targets and no error sorting for 45 min (100,000 counts) in 3D sheath flow sorting system was realized. 3D sheath flow can be focused to 1.2 μm of transverse distribution and 6.0 μm of longitudinal distribution in regard to microbeads of sample flow. The same flow velocity and flow line of the focused sample flow are kept at constant laser irradiation time and power to sort the target. Since sorting was performed in simple and disposable PDMS-glass microchannels without any electric stimulation and mechanical valve structures, the proposed system is suitable for many biochemical applications.

    AB - This paper details a three-dimensional sheath flow sorting microsystem for separation and collection of biomolecules. High-speed particle and biomolecule 3D sheath flow sorting system was realized using reversible thermosensitive hydrogel. The sol-gel transfer of the thermosensitive hydrogel generated by focused IR laser irradiation performs flow switching, the response time of the sol-gel transformation using Mebiol Gel™ was less than 1 ms. For actual bio-applications, accurate and high-speed micro sorter is realized by optimizing the channel structure and by utilizing 3D sheath flow. High-speed sorting less than 3.0 ms from detection to separation of targets and no error sorting for 45 min (100,000 counts) in 3D sheath flow sorting system was realized. 3D sheath flow can be focused to 1.2 μm of transverse distribution and 6.0 μm of longitudinal distribution in regard to microbeads of sample flow. The same flow velocity and flow line of the focused sample flow are kept at constant laser irradiation time and power to sort the target. Since sorting was performed in simple and disposable PDMS-glass microchannels without any electric stimulation and mechanical valve structures, the proposed system is suitable for many biochemical applications.

    KW - Particle and biomolecule sorter

    KW - PDMS microsystem

    KW - Thermosensitive hydrogel

    KW - Three-dimensional sheath flow

    UR - http://www.scopus.com/inward/record.url?scp=33947240882&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=33947240882&partnerID=8YFLogxK

    U2 - 10.1016/j.sna.2006.06.074

    DO - 10.1016/j.sna.2006.06.074

    M3 - Article

    VL - 135

    SP - 99

    EP - 105

    JO - Sensors and Actuators, A: Physical

    JF - Sensors and Actuators, A: Physical

    SN - 0924-4247

    IS - 1

    ER -