Three-dimensional sheath flow sorting microsystem using thermosensitive hydrogel

Takahiro Arakawa, Yoshitaka Shirasaki, Tokihiko Aoki, Takashi Funatsu, Shuichi Shoji

    Research output: Contribution to journalArticle

    31 Citations (Scopus)

    Abstract

    This paper details a three-dimensional sheath flow sorting microsystem for separation and collection of biomolecules. High-speed particle and biomolecule 3D sheath flow sorting system was realized using reversible thermosensitive hydrogel. The sol-gel transfer of the thermosensitive hydrogel generated by focused IR laser irradiation performs flow switching, the response time of the sol-gel transformation using Mebiol Gel™ was less than 1 ms. For actual bio-applications, accurate and high-speed micro sorter is realized by optimizing the channel structure and by utilizing 3D sheath flow. High-speed sorting less than 3.0 ms from detection to separation of targets and no error sorting for 45 min (100,000 counts) in 3D sheath flow sorting system was realized. 3D sheath flow can be focused to 1.2 μm of transverse distribution and 6.0 μm of longitudinal distribution in regard to microbeads of sample flow. The same flow velocity and flow line of the focused sample flow are kept at constant laser irradiation time and power to sort the target. Since sorting was performed in simple and disposable PDMS-glass microchannels without any electric stimulation and mechanical valve structures, the proposed system is suitable for many biochemical applications.

    Original languageEnglish
    Pages (from-to)99-105
    Number of pages7
    JournalSensors and Actuators, A: Physical
    Volume135
    Issue number1
    DOIs
    Publication statusPublished - 2007 Mar 30

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    Keywords

    • Particle and biomolecule sorter
    • PDMS microsystem
    • Thermosensitive hydrogel
    • Three-dimensional sheath flow

    ASJC Scopus subject areas

    • Electrical and Electronic Engineering
    • Mechanical Engineering
    • Instrumentation

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