Thrombopoietin augments stem cell factor-dependent growth of human mast cells from bone marrow multipotential hematopoietic progenitors

Nobukuni Sawai, Kenichi Koike, Hadija Hemed Mwamtemi, Tatsuya Kinoshita, Yumi Kurokawa, Kazuo Sakashita, Tsukasa Higuchi, Kouichi Takeuchi, Masaaki Shiohara, Takehiko Kamijo, Susumu Ito, Takashi Kato, Hiroshi Miyazaki, Tetsuji Yamashita, Atsushi Komiyama

Research output: Contribution to journalArticle

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Abstract

The effects of thrombopoietin (TPO) and/or stem cell factor (SCF) on the development of human mast cells from CD34+ bone marrow (BM) cells were investigated using a serum-deprived liquid culture system. Mast cells were identified by measurement of intracellular histamine content, immunocytochemical staining, and flow cytometric analysis. Whereas SCF alone generated only a small number of tryptase+ cells, the addition of TPO to the culture containing SCF resulted in an apparent production of mast cells from 3 weeks until at least 15 weeks. Some of the cells reacted with an antichymase monoclonal antibody as well. Based on the effects of growth factor(s) on a later phase of the mast cell growth, TPO may stimulate an early stage of mast cell development in combination with SCF, whereas subsequent growth seems to be supported by SCF alone. Single-cell culture studies indicated that the CD34+CD38-c-kit+ cells and CD34+CD38+c-kit+ cells were responsible for the SCF + TPO-dependent mast cell production. Two- step culture assays clearly showed that mast cells originated from multilineage colony-forming cells that had potential to differentiate into neutrophil/mast cell lineages, neutrophil/macrophage/mast cell lineages, or neutrophil/macrophage/mast cell/erythroid lineages. These results suggest that TPO plays an important role in the development of human mast cells from CD34+ BM cells in concert with SCF, and provide direct evidence of the differentiation into the mast cell lineage of human multipotential BM- derived progenitors.

Original languageEnglish
Pages (from-to)3703-3712
Number of pages10
JournalBlood
Volume93
Issue number11
Publication statusPublished - 1999 Jun 1
Externally publishedYes

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Thrombopoietin
Stem Cell Factor
Mast Cells
Bone
Bone Marrow
Growth
Cell Lineage
Macrophages
Cell culture
Neutrophils
Tryptases
Human Development
Bone Marrow Cells
Cell growth
Histamine
Assays
Intercellular Signaling Peptides and Proteins
Monoclonal Antibodies
Cells
Liquids

ASJC Scopus subject areas

  • Hematology

Cite this

Sawai, N., Koike, K., Mwamtemi, H. H., Kinoshita, T., Kurokawa, Y., Sakashita, K., ... Komiyama, A. (1999). Thrombopoietin augments stem cell factor-dependent growth of human mast cells from bone marrow multipotential hematopoietic progenitors. Blood, 93(11), 3703-3712.

Thrombopoietin augments stem cell factor-dependent growth of human mast cells from bone marrow multipotential hematopoietic progenitors. / Sawai, Nobukuni; Koike, Kenichi; Mwamtemi, Hadija Hemed; Kinoshita, Tatsuya; Kurokawa, Yumi; Sakashita, Kazuo; Higuchi, Tsukasa; Takeuchi, Kouichi; Shiohara, Masaaki; Kamijo, Takehiko; Ito, Susumu; Kato, Takashi; Miyazaki, Hiroshi; Yamashita, Tetsuji; Komiyama, Atsushi.

In: Blood, Vol. 93, No. 11, 01.06.1999, p. 3703-3712.

Research output: Contribution to journalArticle

Sawai, N, Koike, K, Mwamtemi, HH, Kinoshita, T, Kurokawa, Y, Sakashita, K, Higuchi, T, Takeuchi, K, Shiohara, M, Kamijo, T, Ito, S, Kato, T, Miyazaki, H, Yamashita, T & Komiyama, A 1999, 'Thrombopoietin augments stem cell factor-dependent growth of human mast cells from bone marrow multipotential hematopoietic progenitors', Blood, vol. 93, no. 11, pp. 3703-3712.
Sawai N, Koike K, Mwamtemi HH, Kinoshita T, Kurokawa Y, Sakashita K et al. Thrombopoietin augments stem cell factor-dependent growth of human mast cells from bone marrow multipotential hematopoietic progenitors. Blood. 1999 Jun 1;93(11):3703-3712.
Sawai, Nobukuni ; Koike, Kenichi ; Mwamtemi, Hadija Hemed ; Kinoshita, Tatsuya ; Kurokawa, Yumi ; Sakashita, Kazuo ; Higuchi, Tsukasa ; Takeuchi, Kouichi ; Shiohara, Masaaki ; Kamijo, Takehiko ; Ito, Susumu ; Kato, Takashi ; Miyazaki, Hiroshi ; Yamashita, Tetsuji ; Komiyama, Atsushi. / Thrombopoietin augments stem cell factor-dependent growth of human mast cells from bone marrow multipotential hematopoietic progenitors. In: Blood. 1999 ; Vol. 93, No. 11. pp. 3703-3712.
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