Thrombopoietin has a direct effect on erythroid differentiation

M. Yamada, N. Komatsu, K. Kirito, R. Shimizu, Takashi Kato, R. Mivazak, P. K. Abe, Y. Miura

Research output: Contribution to journalArticle

Abstract

The administration of thrombopoietin (TPO) to mice after chemotherapy and total body irradiation not only promotes recovery from chemotherapy-induced thrombocytopenia, but also relieves anemia. In addition, TPO has burst promoting activity (BPA) in vitro. These observations suggest the influence of TPO on erythropoiesis. To elucidate whether TPO has a direct effect on erythroid differentiation, we established a subclone of a human cytokine-dependent cell line UT7, UT-7/GM Hl. UT-7/GM HI is maintained by GM-CSF and can grow in response to erythropoietin (EPO) or TPO. After a 1 week culture in IMDM medium containing 10% FCS in the presence of I U/ml of EPO or 10 ng/ml of TPO, more than 90 % and 45 % of UT-7/GM HI cells, respectively, were dianisidine-stained. Even after treatment of UT7/GM HI cells with up to 100 ng/ml of TPO, the EPO receptor was not tyrosine phosphorylated. These findings suggest that TPO directly induced erythroid differentiation and that the effect was not achieved via an erythropoietin receptor.

Original languageEnglish
Pages (from-to)1108
Number of pages1
JournalExperimental Hematology
Volume24
Issue number9
Publication statusPublished - 1996
Externally publishedYes

Fingerprint

Thrombopoietin
Erythropoietin Receptors
Erythropoietin
Dianisidine
Thrombopoietin Receptors
Drug Therapy
Whole-Body Irradiation
Erythropoiesis
Granulocyte-Macrophage Colony-Stimulating Factor
Thrombocytopenia
Tyrosine
Anemia
Cytokines
Cell Line

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

Cite this

Yamada, M., Komatsu, N., Kirito, K., Shimizu, R., Kato, T., Mivazak, R., ... Miura, Y. (1996). Thrombopoietin has a direct effect on erythroid differentiation. Experimental Hematology, 24(9), 1108.

Thrombopoietin has a direct effect on erythroid differentiation. / Yamada, M.; Komatsu, N.; Kirito, K.; Shimizu, R.; Kato, Takashi; Mivazak, R.; Abe, P. K.; Miura, Y.

In: Experimental Hematology, Vol. 24, No. 9, 1996, p. 1108.

Research output: Contribution to journalArticle

Yamada, M, Komatsu, N, Kirito, K, Shimizu, R, Kato, T, Mivazak, R, Abe, PK & Miura, Y 1996, 'Thrombopoietin has a direct effect on erythroid differentiation', Experimental Hematology, vol. 24, no. 9, pp. 1108.
Yamada M, Komatsu N, Kirito K, Shimizu R, Kato T, Mivazak R et al. Thrombopoietin has a direct effect on erythroid differentiation. Experimental Hematology. 1996;24(9):1108.
Yamada, M. ; Komatsu, N. ; Kirito, K. ; Shimizu, R. ; Kato, Takashi ; Mivazak, R. ; Abe, P. K. ; Miura, Y. / Thrombopoietin has a direct effect on erythroid differentiation. In: Experimental Hematology. 1996 ; Vol. 24, No. 9. pp. 1108.
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