Thrombopoietin induces production of nucleated thrombocytes from liver cells in Xenopus laevis

Yuta Tanizaki, Megumi Ichisugi, Miyako Obuchi-Shimoji, Takako Ishida-Iwata, Ayaka Tahara-Mogi, Mizue Meguro-Ishikawa, Takashi Kato

    Research output: Contribution to journalArticle

    5 Citations (Scopus)

    Abstract

    The development of mammalian megakaryocytes (MKs) and platelets, which are thought to be absent in non-mammals, is primarily regulated by the thrombopoietin (TPO)/Mpl system. Although non-mammals possess nucleated thrombocytes instead of platelets, the features of nucleated thrombocyte progenitors remain to be clarified. Here, we provide the general features of TPO using Xenopus laevis TPO (xlTPO). Hepatic and splenic cells were cultured in liquid suspension with recombinant xlTPO. These cells differentiated into large, round, polyploid CD41-expressing cells and were classified as X. laevis MKs, comparable to mammalian MKs. The subsequent culture of MKs after removal of xlTPO produced mature, spindle-shaped thrombocytes that were activated by thrombin, thereby altering their morphology. XlTPO induced MKs in cultured hepatic cells for at least three weeks; however, this was not observed in splenic cells; this result demonstrates the origin of early haematopoietic progenitors in the liver rather than the spleen. Additionally, xlTPO enhanced viability of peripheral thrombocytes, indicating the xlTPO-Mpl pathway stimulates anti-apoptotic in peripheral thrombocytes. The development of thrombocytes from MKs via the TPO-Mpl system in X. laevis plays a crucial role in their development from MKs, comparable to mammalian thrombopoiesis. Thus, our results offer insight into the cellular evolution of platelets/MKs in vertebrates. (200/200).

    Original languageEnglish
    Article number18519
    JournalScientific Reports
    Volume5
    DOIs
    Publication statusPublished - 2015 Dec 21

    ASJC Scopus subject areas

    • General

    Fingerprint Dive into the research topics of 'Thrombopoietin induces production of nucleated thrombocytes from liver cells in Xenopus laevis'. Together they form a unique fingerprint.

  • Cite this

    Tanizaki, Y., Ichisugi, M., Obuchi-Shimoji, M., Ishida-Iwata, T., Tahara-Mogi, A., Meguro-Ishikawa, M., & Kato, T. (2015). Thrombopoietin induces production of nucleated thrombocytes from liver cells in Xenopus laevis. Scientific Reports, 5, [18519]. https://doi.org/10.1038/srep18519