Thrombopoietin Induces Tyrosine Phosphorylation and Activation of Mitogen-Activated Protein Kinases in a Human Thrombopoietin-Dependent Cell Line

M. Yamada, N. Komatsu, K. Okada, Takashi Kato, H. Miyazaki, Y. Miura

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Thrombopoietin (TPO) is a cytokine which can support the proliferation and differentiation of megakaryocyte progenitor cells, and the maturation of megakaryocytes. We show here that mitogen-activated protein (MAP) kinases, Erk1 and Erk2, are involved in TPO signal transduction in the human TPO-dependent megakaryocytic cell line, UT-7/TPO. TPO induced tyrosine phosphorylation of Erk1 and Erk2 proteins in a dose and time-dependent manner. Moreover, the activation of MAP kinases was actually induced by TPO. These results suggest that MAP kinase activation is involved in the signalling pathway of TPO, as it is for other cytokines, one of which is erythropoietin.

Original languageEnglish
Pages (from-to)230-237
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume217
Issue number1
DOIs
Publication statusPublished - 1995 Dec 5
Externally publishedYes

Fingerprint

Thrombopoietin
Phosphorylation
Mitogen-Activated Protein Kinases
Tyrosine
Chemical activation
Cells
Cell Line
Megakaryocyte Progenitor Cells
Cytokines
Signal transduction
Megakaryocytes
Erythropoietin
Signal Transduction

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology
  • Biophysics
  • Biochemistry

Cite this

Thrombopoietin Induces Tyrosine Phosphorylation and Activation of Mitogen-Activated Protein Kinases in a Human Thrombopoietin-Dependent Cell Line. / Yamada, M.; Komatsu, N.; Okada, K.; Kato, Takashi; Miyazaki, H.; Miura, Y.

In: Biochemical and Biophysical Research Communications, Vol. 217, No. 1, 05.12.1995, p. 230-237.

Research output: Contribution to journalArticle

@article{e589da91b99d4ca88f44b933c5514ef1,
title = "Thrombopoietin Induces Tyrosine Phosphorylation and Activation of Mitogen-Activated Protein Kinases in a Human Thrombopoietin-Dependent Cell Line",
abstract = "Thrombopoietin (TPO) is a cytokine which can support the proliferation and differentiation of megakaryocyte progenitor cells, and the maturation of megakaryocytes. We show here that mitogen-activated protein (MAP) kinases, Erk1 and Erk2, are involved in TPO signal transduction in the human TPO-dependent megakaryocytic cell line, UT-7/TPO. TPO induced tyrosine phosphorylation of Erk1 and Erk2 proteins in a dose and time-dependent manner. Moreover, the activation of MAP kinases was actually induced by TPO. These results suggest that MAP kinase activation is involved in the signalling pathway of TPO, as it is for other cytokines, one of which is erythropoietin.",
author = "M. Yamada and N. Komatsu and K. Okada and Takashi Kato and H. Miyazaki and Y. Miura",
year = "1995",
month = "12",
day = "5",
doi = "10.1006/bbrc.1995.2768",
language = "English",
volume = "217",
pages = "230--237",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Thrombopoietin Induces Tyrosine Phosphorylation and Activation of Mitogen-Activated Protein Kinases in a Human Thrombopoietin-Dependent Cell Line

AU - Yamada, M.

AU - Komatsu, N.

AU - Okada, K.

AU - Kato, Takashi

AU - Miyazaki, H.

AU - Miura, Y.

PY - 1995/12/5

Y1 - 1995/12/5

N2 - Thrombopoietin (TPO) is a cytokine which can support the proliferation and differentiation of megakaryocyte progenitor cells, and the maturation of megakaryocytes. We show here that mitogen-activated protein (MAP) kinases, Erk1 and Erk2, are involved in TPO signal transduction in the human TPO-dependent megakaryocytic cell line, UT-7/TPO. TPO induced tyrosine phosphorylation of Erk1 and Erk2 proteins in a dose and time-dependent manner. Moreover, the activation of MAP kinases was actually induced by TPO. These results suggest that MAP kinase activation is involved in the signalling pathway of TPO, as it is for other cytokines, one of which is erythropoietin.

AB - Thrombopoietin (TPO) is a cytokine which can support the proliferation and differentiation of megakaryocyte progenitor cells, and the maturation of megakaryocytes. We show here that mitogen-activated protein (MAP) kinases, Erk1 and Erk2, are involved in TPO signal transduction in the human TPO-dependent megakaryocytic cell line, UT-7/TPO. TPO induced tyrosine phosphorylation of Erk1 and Erk2 proteins in a dose and time-dependent manner. Moreover, the activation of MAP kinases was actually induced by TPO. These results suggest that MAP kinase activation is involved in the signalling pathway of TPO, as it is for other cytokines, one of which is erythropoietin.

UR - http://www.scopus.com/inward/record.url?scp=0029584186&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029584186&partnerID=8YFLogxK

U2 - 10.1006/bbrc.1995.2768

DO - 10.1006/bbrc.1995.2768

M3 - Article

VL - 217

SP - 230

EP - 237

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 1

ER -