Thrombopoietin induces tyrosine phosphorylation and activation of mitogen-activated protein kinases in a human thrombopoietin-dependent cell line

Minami Yamada, Norio Komatsu, Koji Okada, Takashi Kato, Hiroshi Miyazaki, Yasusada Miura

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46 Citations (Scopus)

Abstract

Thrombopoietin (TPO) is a cytokine which can support the proliferation and differentiation of megakaryocyte progenitor cells, and the maturation of megakaryocytes. We show here that mitogen-activated protein (MAP) kinases, Erk1 and Erk2, are involved in TPO signal transduction in the human TPO-dependent megakaryocytic cell line, UT-7/TPO. TPO induced tyrosine phosphorylation of Erk1 and Erk2 proteins in a dose and time-dependent manner. Moreover, the activation of MAP kinases was actually induced by TPO. These results suggest that MAP kinase activation is involved in the signalling pathway of TPO, as it is for other cytokines, one of which is erythropoietin.

Original languageEnglish
Pages (from-to)230-237
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume217
Issue number1
DOIs
Publication statusPublished - 1995 Jan 1

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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