Thrombopoietin stimulates proliferation and megakaryocytic differentiation of mouse pro-B cell line BF-TE22

Hideya Ohashi, Haruhiko Morita, Tomoyuki Tahara, Hikaru Tsunakawa, Atsushi Matsumoto, Kinya Ogami, Takashi Kato, Hiroshi Miyazaki

Research output: Contribution to journalArticle

Abstract

We have isolated and characterized a thrombopoietin (TPO)-dependent BF-TE22 cell line endogenously expressing murine Mp1, which is a subclone of murine pro-B Ba/F3 cells. TPO stimulated the proliferation of BF-TE22 cells in a dose-dependent manner, and also induced the expression of megakaryocyte lineage-specific AP-51 and CD61 cell surface antigens. The results indicate that the murine Mp1 on BF-TE22 cells can transmit both proliferation and megakaryocyte lineage-specific differentiation signals to cells. Furthermore, it was shown that IL-3 inhibits the TPO-induced differentiation signals of BF-TE22 cells. These results suggest that the signals mediated by IL-3 predominate over thor of TPO in BF-TE22 cells. Thus, BF-TE22 cells will be useful for the biological and biochemical studies of the TPO-Mp1 signal transduction mechanism.

Original languageEnglish
Pages (from-to)199-206
Number of pages8
JournalCytotechnology
Volume26
Issue number3
DOIs
Publication statusPublished - 1998
Externally publishedYes

Fingerprint

Thrombopoietin
Signal transduction
B-Lymphoid Precursor Cells
Antigens
Cells
Cell Line
Interleukin-3
Megakaryocytes
Surface Antigens
Signal Transduction

Keywords

  • Differentiation
  • Proliferation
  • Thrombopoietin

ASJC Scopus subject areas

  • Biotechnology
  • Cell Biology
  • Clinical Biochemistry

Cite this

Ohashi, H., Morita, H., Tahara, T., Tsunakawa, H., Matsumoto, A., Ogami, K., ... Miyazaki, H. (1998). Thrombopoietin stimulates proliferation and megakaryocytic differentiation of mouse pro-B cell line BF-TE22. Cytotechnology, 26(3), 199-206. https://doi.org/10.1023/A:1007915809529

Thrombopoietin stimulates proliferation and megakaryocytic differentiation of mouse pro-B cell line BF-TE22. / Ohashi, Hideya; Morita, Haruhiko; Tahara, Tomoyuki; Tsunakawa, Hikaru; Matsumoto, Atsushi; Ogami, Kinya; Kato, Takashi; Miyazaki, Hiroshi.

In: Cytotechnology, Vol. 26, No. 3, 1998, p. 199-206.

Research output: Contribution to journalArticle

Ohashi, H, Morita, H, Tahara, T, Tsunakawa, H, Matsumoto, A, Ogami, K, Kato, T & Miyazaki, H 1998, 'Thrombopoietin stimulates proliferation and megakaryocytic differentiation of mouse pro-B cell line BF-TE22', Cytotechnology, vol. 26, no. 3, pp. 199-206. https://doi.org/10.1023/A:1007915809529
Ohashi, Hideya ; Morita, Haruhiko ; Tahara, Tomoyuki ; Tsunakawa, Hikaru ; Matsumoto, Atsushi ; Ogami, Kinya ; Kato, Takashi ; Miyazaki, Hiroshi. / Thrombopoietin stimulates proliferation and megakaryocytic differentiation of mouse pro-B cell line BF-TE22. In: Cytotechnology. 1998 ; Vol. 26, No. 3. pp. 199-206.
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