Thrombopoietin stimulates proliferation and megakaryocytic differentiation of mouse pro-B cell line BF-TE22

Hideya Ohashi, Haruhiko Morita, Tomoyuki Tahara, Hikaru Tsunakawa, Atsushi Matsumoto, Kinya Ogami, Takashi Kato, Hiroshi Miyazaki

Research output: Contribution to journalArticle

Abstract

We have isolated and characterized a thrombopoietin (TPO)-dependent BF-TE22 cell line endogenously expressing murine Mp1, which is a subclone of murine pro-B Ba/F3 cells. TPO stimulated the proliferation of BF-TE22 cells in a dose-dependent manner, and also induced the expression of megakaryocyte lineage-specific AP-51 and CD61 cell surface antigens. The results indicate that the murine Mp1 on BF-TE22 cells can transmit both proliferation and megakaryocyte lineage-specific differentiation signals to cells. Furthermore, it was shown that IL-3 inhibits the TPO-induced differentiation signals of BF-TE22 cells. These results suggest that the signals mediated by IL-3 predominate over thor of TPO in BF-TE22 cells. Thus, BF-TE22 cells will be useful for the biological and biochemical studies of the TPO-Mp1 signal transduction mechanism.

Original languageEnglish
Pages (from-to)199-206
Number of pages8
JournalCytotechnology
Volume26
Issue number3
DOIs
Publication statusPublished - 1998
Externally publishedYes

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Keywords

  • Differentiation
  • Proliferation
  • Thrombopoietin

ASJC Scopus subject areas

  • Biotechnology
  • Cell Biology
  • Clinical Biochemistry

Cite this

Ohashi, H., Morita, H., Tahara, T., Tsunakawa, H., Matsumoto, A., Ogami, K., Kato, T., & Miyazaki, H. (1998). Thrombopoietin stimulates proliferation and megakaryocytic differentiation of mouse pro-B cell line BF-TE22. Cytotechnology, 26(3), 199-206. https://doi.org/10.1023/A:1007915809529