Thyrotropin-Releasing Hormone (TRH) Is the Major Prolactin-Releasing Factor in the Bullfrog Hypothalamus

Keisuke Nakajima, Daisuke Uchida, Makoto Sakai, Noriyuki Takahashi, Tadashi Yanagisawa, Kazutoshi Yamamoto, Sakae Kikuyama

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    Abstract

    A substance exhibiting potent activity in stimulating the release of prolactin from bullfrog (Rana catesbeiana) pituitary in vitro was isolated from an acid extract of bullfrog hypothalami by gel-filtration chromatography (Sephadex G-15), ion-exchange chromatography (Mono-S HR 5/5), and reverse-phase high-performance liquid chromatography (TSK-gel ODS-120T). Its amino acid composition was similar to that of synthetic thyrotropin-releasing hormone (TRH). Radioimmunoassay confirmed that the substance had TRH immunoreactivity. Moreover, it exhibited the same chromatographic behavior as that of synthetic TRH. These results clearly indicate that the isolated hypothalamic substance is TRH, and that it is the major prolactin-releasing factor present in the bullfrog hypothalamus.

    Original languageEnglish
    Pages (from-to)11-16
    Number of pages6
    JournalGeneral and Comparative Endocrinology
    Volume89
    Issue number1
    DOIs
    Publication statusPublished - 1993 Jan

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    ASJC Scopus subject areas

    • Endocrinology

    Cite this

    Nakajima, K., Uchida, D., Sakai, M., Takahashi, N., Yanagisawa, T., Yamamoto, K., & Kikuyama, S. (1993). Thyrotropin-Releasing Hormone (TRH) Is the Major Prolactin-Releasing Factor in the Bullfrog Hypothalamus. General and Comparative Endocrinology, 89(1), 11-16. https://doi.org/10.1006/gcen.1993.1004