TIPS pentacene loaded PEO-PDLLA core-shell nanoparticles have similar cellular uptake dynamics in M1 and M2 macrophages and in corresponding in vivo microenvironments

Dylan K. McDaniel, Ami Jo, Veronica M. Ringel-Scaia, Sheryl Coutermarsh-Ott, Daniel E. Rothschild, Michael D. Powell, Rui Zhang, Timothy Edward Long, Kenneth J. Oestreich, Judy S. Riffle, Richey M. Davis, Irving C. Allen

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Nanoparticle based drug delivery platforms have the potential to transform disease treatment paradigms and therapeutic strategies, especially in the context of pulmonary medicine. Once administered, nanoparticles disperse throughout the lung and many are phagocytosed by macrophages. However, there is a paucity of knowledge regarding cellular up-take dynamics of nanoparticles due largely to macrophage heterogeneity. To address this issue, we sought to better define nanoparticle up-take using polarized M1 and M2 macrophages and novel TIPS-pentacene loaded PEO-PDLLA nanoparticles. Our data reveal that primary macrophages polarized to either M1 or M2 phenotypes have similar levels of nanoparticle phagocytosis. Similarly, M1 and M2 polarized macrophages isolated from the lungs of mice following either acute (Th1) or allergic (Th2) airway inflammation also demonstrated equivalent levels of nanoparticle up-take. Together, these studies provide critical benchmark information pertaining to cellular up-take dynamics and biodistribution of nanoparticles in the context of clinically relevant inflammatory microenvironments.

Original languageEnglish
Pages (from-to)1255-1266
Number of pages12
JournalNanomedicine: Nanotechnology, Biology, and Medicine
Volume13
Issue number3
DOIs
Publication statusPublished - 2017 Apr 1
Externally publishedYes

Fingerprint

Macrophages
Polyethylene oxides
Nanoparticles
Phagocytosis
Benchmarking
Pulmonary Medicine
Lung
bis(triisopropylsilylethynyl)pentacene
Drug delivery
Medicine
Inflammation
Phenotype
Pharmaceutical Preparations

Keywords

  • Asthma
  • Inflammation
  • LPS
  • Nanoparticle
  • Pulmonary drug delivery

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Molecular Medicine
  • Biomedical Engineering
  • Materials Science(all)
  • Pharmaceutical Science

Cite this

TIPS pentacene loaded PEO-PDLLA core-shell nanoparticles have similar cellular uptake dynamics in M1 and M2 macrophages and in corresponding in vivo microenvironments. / McDaniel, Dylan K.; Jo, Ami; Ringel-Scaia, Veronica M.; Coutermarsh-Ott, Sheryl; Rothschild, Daniel E.; Powell, Michael D.; Zhang, Rui; Long, Timothy Edward; Oestreich, Kenneth J.; Riffle, Judy S.; Davis, Richey M.; Allen, Irving C.

In: Nanomedicine: Nanotechnology, Biology, and Medicine, Vol. 13, No. 3, 01.04.2017, p. 1255-1266.

Research output: Contribution to journalArticle

McDaniel, DK, Jo, A, Ringel-Scaia, VM, Coutermarsh-Ott, S, Rothschild, DE, Powell, MD, Zhang, R, Long, TE, Oestreich, KJ, Riffle, JS, Davis, RM & Allen, IC 2017, 'TIPS pentacene loaded PEO-PDLLA core-shell nanoparticles have similar cellular uptake dynamics in M1 and M2 macrophages and in corresponding in vivo microenvironments', Nanomedicine: Nanotechnology, Biology, and Medicine, vol. 13, no. 3, pp. 1255-1266. https://doi.org/10.1016/j.nano.2016.12.015
McDaniel, Dylan K. ; Jo, Ami ; Ringel-Scaia, Veronica M. ; Coutermarsh-Ott, Sheryl ; Rothschild, Daniel E. ; Powell, Michael D. ; Zhang, Rui ; Long, Timothy Edward ; Oestreich, Kenneth J. ; Riffle, Judy S. ; Davis, Richey M. ; Allen, Irving C. / TIPS pentacene loaded PEO-PDLLA core-shell nanoparticles have similar cellular uptake dynamics in M1 and M2 macrophages and in corresponding in vivo microenvironments. In: Nanomedicine: Nanotechnology, Biology, and Medicine. 2017 ; Vol. 13, No. 3. pp. 1255-1266.
@article{2c832b4a271844e8a4a665e7d48dfff2,
title = "TIPS pentacene loaded PEO-PDLLA core-shell nanoparticles have similar cellular uptake dynamics in M1 and M2 macrophages and in corresponding in vivo microenvironments",
abstract = "Nanoparticle based drug delivery platforms have the potential to transform disease treatment paradigms and therapeutic strategies, especially in the context of pulmonary medicine. Once administered, nanoparticles disperse throughout the lung and many are phagocytosed by macrophages. However, there is a paucity of knowledge regarding cellular up-take dynamics of nanoparticles due largely to macrophage heterogeneity. To address this issue, we sought to better define nanoparticle up-take using polarized M1 and M2 macrophages and novel TIPS-pentacene loaded PEO-PDLLA nanoparticles. Our data reveal that primary macrophages polarized to either M1 or M2 phenotypes have similar levels of nanoparticle phagocytosis. Similarly, M1 and M2 polarized macrophages isolated from the lungs of mice following either acute (Th1) or allergic (Th2) airway inflammation also demonstrated equivalent levels of nanoparticle up-take. Together, these studies provide critical benchmark information pertaining to cellular up-take dynamics and biodistribution of nanoparticles in the context of clinically relevant inflammatory microenvironments.",
keywords = "Asthma, Inflammation, LPS, Nanoparticle, Pulmonary drug delivery",
author = "McDaniel, {Dylan K.} and Ami Jo and Ringel-Scaia, {Veronica M.} and Sheryl Coutermarsh-Ott and Rothschild, {Daniel E.} and Powell, {Michael D.} and Rui Zhang and Long, {Timothy Edward} and Oestreich, {Kenneth J.} and Riffle, {Judy S.} and Davis, {Richey M.} and Allen, {Irving C.}",
year = "2017",
month = "4",
day = "1",
doi = "10.1016/j.nano.2016.12.015",
language = "English",
volume = "13",
pages = "1255--1266",
journal = "Nanomedicine: Nanotechnology, Biology, and Medicine",
issn = "1549-9634",
publisher = "Elsevier Inc.",
number = "3",

}

TY - JOUR

T1 - TIPS pentacene loaded PEO-PDLLA core-shell nanoparticles have similar cellular uptake dynamics in M1 and M2 macrophages and in corresponding in vivo microenvironments

AU - McDaniel, Dylan K.

AU - Jo, Ami

AU - Ringel-Scaia, Veronica M.

AU - Coutermarsh-Ott, Sheryl

AU - Rothschild, Daniel E.

AU - Powell, Michael D.

AU - Zhang, Rui

AU - Long, Timothy Edward

AU - Oestreich, Kenneth J.

AU - Riffle, Judy S.

AU - Davis, Richey M.

AU - Allen, Irving C.

PY - 2017/4/1

Y1 - 2017/4/1

N2 - Nanoparticle based drug delivery platforms have the potential to transform disease treatment paradigms and therapeutic strategies, especially in the context of pulmonary medicine. Once administered, nanoparticles disperse throughout the lung and many are phagocytosed by macrophages. However, there is a paucity of knowledge regarding cellular up-take dynamics of nanoparticles due largely to macrophage heterogeneity. To address this issue, we sought to better define nanoparticle up-take using polarized M1 and M2 macrophages and novel TIPS-pentacene loaded PEO-PDLLA nanoparticles. Our data reveal that primary macrophages polarized to either M1 or M2 phenotypes have similar levels of nanoparticle phagocytosis. Similarly, M1 and M2 polarized macrophages isolated from the lungs of mice following either acute (Th1) or allergic (Th2) airway inflammation also demonstrated equivalent levels of nanoparticle up-take. Together, these studies provide critical benchmark information pertaining to cellular up-take dynamics and biodistribution of nanoparticles in the context of clinically relevant inflammatory microenvironments.

AB - Nanoparticle based drug delivery platforms have the potential to transform disease treatment paradigms and therapeutic strategies, especially in the context of pulmonary medicine. Once administered, nanoparticles disperse throughout the lung and many are phagocytosed by macrophages. However, there is a paucity of knowledge regarding cellular up-take dynamics of nanoparticles due largely to macrophage heterogeneity. To address this issue, we sought to better define nanoparticle up-take using polarized M1 and M2 macrophages and novel TIPS-pentacene loaded PEO-PDLLA nanoparticles. Our data reveal that primary macrophages polarized to either M1 or M2 phenotypes have similar levels of nanoparticle phagocytosis. Similarly, M1 and M2 polarized macrophages isolated from the lungs of mice following either acute (Th1) or allergic (Th2) airway inflammation also demonstrated equivalent levels of nanoparticle up-take. Together, these studies provide critical benchmark information pertaining to cellular up-take dynamics and biodistribution of nanoparticles in the context of clinically relevant inflammatory microenvironments.

KW - Asthma

KW - Inflammation

KW - LPS

KW - Nanoparticle

KW - Pulmonary drug delivery

UR - http://www.scopus.com/inward/record.url?scp=85013857884&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85013857884&partnerID=8YFLogxK

U2 - 10.1016/j.nano.2016.12.015

DO - 10.1016/j.nano.2016.12.015

M3 - Article

C2 - 28040495

AN - SCOPUS:85013857884

VL - 13

SP - 1255

EP - 1266

JO - Nanomedicine: Nanotechnology, Biology, and Medicine

JF - Nanomedicine: Nanotechnology, Biology, and Medicine

SN - 1549-9634

IS - 3

ER -