TIPS pentacene loaded PEO-PDLLA core-shell nanoparticles have similar cellular uptake dynamics in M1 and M2 macrophages and in corresponding in vivo microenvironments

Dylan K. McDaniel, Ami Jo, Veronica M. Ringel-Scaia, Sheryl Coutermarsh-Ott, Daniel E. Rothschild, Michael D. Powell, Rui Zhang, Timothy Edward Long, Kenneth J. Oestreich, Judy S. Riffle, Richey M. Davis, Irving C. Allen

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Nanoparticle based drug delivery platforms have the potential to transform disease treatment paradigms and therapeutic strategies, especially in the context of pulmonary medicine. Once administered, nanoparticles disperse throughout the lung and many are phagocytosed by macrophages. However, there is a paucity of knowledge regarding cellular up-take dynamics of nanoparticles due largely to macrophage heterogeneity. To address this issue, we sought to better define nanoparticle up-take using polarized M1 and M2 macrophages and novel TIPS-pentacene loaded PEO-PDLLA nanoparticles. Our data reveal that primary macrophages polarized to either M1 or M2 phenotypes have similar levels of nanoparticle phagocytosis. Similarly, M1 and M2 polarized macrophages isolated from the lungs of mice following either acute (Th1) or allergic (Th2) airway inflammation also demonstrated equivalent levels of nanoparticle up-take. Together, these studies provide critical benchmark information pertaining to cellular up-take dynamics and biodistribution of nanoparticles in the context of clinically relevant inflammatory microenvironments.

Original languageEnglish
Pages (from-to)1255-1266
Number of pages12
JournalNanomedicine: Nanotechnology, Biology, and Medicine
Volume13
Issue number3
DOIs
Publication statusPublished - 2017 Apr 1
Externally publishedYes

Keywords

  • Asthma
  • Inflammation
  • LPS
  • Nanoparticle
  • Pulmonary drug delivery

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Molecular Medicine
  • Biomedical Engineering
  • Materials Science(all)
  • Pharmaceutical Science

Fingerprint Dive into the research topics of 'TIPS pentacene loaded PEO-PDLLA core-shell nanoparticles have similar cellular uptake dynamics in M1 and M2 macrophages and in corresponding in vivo microenvironments'. Together they form a unique fingerprint.

Cite this