Toll-like receptor 4 is involved in titanium dioxide nanoparticle incorporation into cells

Sharmy Saimon Mano, Koki Kanehira, Shuji Sonezaki, Akiyoshi Taniguchi*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


The innate immune system is important for defense against foreign pathogens, such as bacteria and viruses. We hypothesized that the innate immune system is also important for the interaction between cells and nanomaterials, because cells do not have a specific defense system for nanomaterials. In this study, we demonstrated the effect of Toll-Like Receptor 4 (TLR 4), which is an important molecule for the innate immune system, on the uptake of TiO2 nanoparticles (NPs). The results indicated that TLR 4 expression vector-transfected cells increased the uptake of TiO2 NPs and also increased IL-6 mRNA induction by TiO2 NP exposure. Modifying TiO2 NPs with polyethylene glycol (PEG) reduced their uptake and inflammatory-signal transduction via TLR 4. Our results suggest that TLR 4 is important for interactions between cells and NPs, and that PEGylation of TiO2 NPs reduces the cellular response via interaction with TLR 4.

Original languageEnglish
Pages (from-to)1562-1568
Number of pages7
JournalScience of Advanced Materials
Issue number7
Publication statusPublished - 2014 Jul


  • Gene expression
  • Innate immunity
  • Poly ethylene glycol
  • Side scatter
  • Titanium dioxide

ASJC Scopus subject areas

  • Materials Science(all)


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