Toll-like receptor 4 is involved in titanium dioxide nanoparticle incorporation into cells

Sharmy Saimon Mano, Koki Kanehira, Shuji Sonezaki, Akiyoshi Taniguchi

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    The innate immune system is important for defense against foreign pathogens, such as bacteria and viruses. We hypothesized that the innate immune system is also important for the interaction between cells and nanomaterials, because cells do not have a specific defense system for nanomaterials. In this study, we demonstrated the effect of Toll-Like Receptor 4 (TLR 4), which is an important molecule for the innate immune system, on the uptake of TiO2 nanoparticles (NPs). The results indicated that TLR 4 expression vector-transfected cells increased the uptake of TiO2 NPs and also increased IL-6 mRNA induction by TiO2 NP exposure. Modifying TiO2 NPs with polyethylene glycol (PEG) reduced their uptake and inflammatory-signal transduction via TLR 4. Our results suggest that TLR 4 is important for interactions between cells and NPs, and that PEGylation of TiO2 NPs reduces the cellular response via interaction with TLR 4.

    Original languageEnglish
    Pages (from-to)1562-1568
    Number of pages7
    JournalScience of Advanced Materials
    Volume6
    Issue number7
    DOIs
    Publication statusPublished - 2014

    Fingerprint

    Toll-Like Receptor 4
    Titanium dioxide
    Nanoparticles
    Immune system
    Nanostructured materials
    Signal transduction
    Pathogens
    Viruses
    Polyethylene glycols
    titanium dioxide
    Interleukin-6
    Bacteria
    Messenger RNA
    Molecules

    Keywords

    • Gene expression
    • Innate immunity
    • Poly ethylene glycol
    • Side scatter
    • Titanium dioxide

    ASJC Scopus subject areas

    • Materials Science(all)

    Cite this

    Toll-like receptor 4 is involved in titanium dioxide nanoparticle incorporation into cells. / Mano, Sharmy Saimon; Kanehira, Koki; Sonezaki, Shuji; Taniguchi, Akiyoshi.

    In: Science of Advanced Materials, Vol. 6, No. 7, 2014, p. 1562-1568.

    Research output: Contribution to journalArticle

    Mano, Sharmy Saimon ; Kanehira, Koki ; Sonezaki, Shuji ; Taniguchi, Akiyoshi. / Toll-like receptor 4 is involved in titanium dioxide nanoparticle incorporation into cells. In: Science of Advanced Materials. 2014 ; Vol. 6, No. 7. pp. 1562-1568.
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