Towards single-cell genome analysis of circulating tumor cells based on microcavity array

Tomoko Yoshino, Hisashige Kanbara, Ryo Negishi, Kaori Takai, Tadashi Matsunaga, Tsuyoshi Tanaka

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Circulating tumor cells (CTCs) are defined as tumor cells circulating in the peripheral blood of patients with metastatic cancer. CTCs are well recognized as accessible and valuable biomarkers for cancer diagnosis and are a potential target for drug discovery. However, the recovery of such extremely rare CTCs from the peripheral blood, which contains a mixture of CTCs and a large number of hematologic cells, presents a technical challenge. In this study, techniques for the recovery of CTCs based on the microcavity array (MCA) are introduced. The MCA can specifically obtain tumor cells from whole blood based on differences in the size and deformability between tumor cells and hematologic cells. By controlling the size and shape of the cavity, efficient CTC recovery was achieved in our study. Furthermore, an automated CTC recovery system using a MCA was developed for subsequent high-throughput analysis. The MCA system was finally applied to single-cell genome analysis, and accurate single-cell manipulation and genome amplification without any contamination of neighboring cells and without loss of target cells was achieved.

Original languageEnglish
Title of host publication2016 World Automation Congress, WAC 2016
PublisherIEEE Computer Society
Volume2016-October
ISBN (Electronic)9781889335513
DOIs
Publication statusPublished - 2016 Oct 4
Externally publishedYes
Event2016 World Automation Congress, WAC 2016 - Rio Grande, United States
Duration: 2016 Jul 312016 Aug 4

Other

Other2016 World Automation Congress, WAC 2016
CountryUnited States
CityRio Grande
Period16/7/3116/8/4

Keywords

  • Circulating tumor cells (CTCs)
  • hydrogel
  • microcavity array (MCA)
  • single cell

ASJC Scopus subject areas

  • Control and Systems Engineering

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  • Cite this

    Yoshino, T., Kanbara, H., Negishi, R., Takai, K., Matsunaga, T., & Tanaka, T. (2016). Towards single-cell genome analysis of circulating tumor cells based on microcavity array. In 2016 World Automation Congress, WAC 2016 (Vol. 2016-October). [7583055] IEEE Computer Society. https://doi.org/10.1109/WAC.2016.7583055