Towards the development of a non-bioptic diagnostic technique for eosinophilic esophagitis using Raman spectroscopy

Hemanth Noothalapati, Suguru Uemura, Naoki Ohshima, Yoshikazu Kinoshita, Masahiro Ando, Hiro O. Hamaguchi, Tatsuyuki Yamamoto

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Eosinophilic esophagitis (EoE) is believed to be a rare pathological condition that is characterized by dense infiltration of eosinophils in esophageal epithelial layer. Occurrence of this disease worldwide has started increasing rapidly in the last decade. Routine endoscopic observations can help in diagnosis only if clear longitudinal furrows or multiple concentric rings are observed but does not give any definitive conclusion in the early stages. Hence esophageal tissue samples are collected from multiple sites by biopsy and the number of eosinophils is counted after staining. Such a procedure is time consuming and has an inherent risk of bleeding, eventually damaging esophagus. Hence we developed a resonance Raman spectroscopy based approach to detect eosinophils in esophagus using mouse models. Our results show the presence of eosinophils in mice esophageal tissues suffering from inflammation by administering interleukin-33 while there are none in control mice. We believe this method can be used in clinical application for diagnosing EoE in the near future.

Original languageEnglish
Pages (from-to)7-10
Number of pages4
JournalVibrational Spectroscopy
Volume85
DOIs
Publication statusPublished - 2016 Jul 1

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Raman spectroscopy
Tissue
Biopsy
Infiltration
Interleukin-33

Keywords

  • Eosinophil peroxidase
  • Eosinophilic esophagitis
  • Non-bioptic diagnosis
  • Raman spectroscopy

ASJC Scopus subject areas

  • Spectroscopy

Cite this

Towards the development of a non-bioptic diagnostic technique for eosinophilic esophagitis using Raman spectroscopy. / Noothalapati, Hemanth; Uemura, Suguru; Ohshima, Naoki; Kinoshita, Yoshikazu; Ando, Masahiro; Hamaguchi, Hiro O.; Yamamoto, Tatsuyuki.

In: Vibrational Spectroscopy, Vol. 85, 01.07.2016, p. 7-10.

Research output: Contribution to journalArticle

Noothalapati, Hemanth ; Uemura, Suguru ; Ohshima, Naoki ; Kinoshita, Yoshikazu ; Ando, Masahiro ; Hamaguchi, Hiro O. ; Yamamoto, Tatsuyuki. / Towards the development of a non-bioptic diagnostic technique for eosinophilic esophagitis using Raman spectroscopy. In: Vibrational Spectroscopy. 2016 ; Vol. 85. pp. 7-10.
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