Tracking epigenetic histone modifications in single cells using Fab-based live endogenous modification labeling

Yoko Hayashi-Takanaka, Kazuo Yamagata, Teruhiko Wakayama, Timothy J. Stasevich, Takashi Kainuma, Toshiki Tsurimoto, Makoto Tachibana, Yoichi Shinkai, Hitoshi Kurumizaka, Naohito Nozaki, Hiroshi Kimura

    Research output: Contribution to journalArticle

    124 Citations (Scopus)

    Abstract

    Histone modifications play an important role in epigenetic gene regulation and genome integrity. It remains largely unknown, however, how these modifications dynamically change in individual cells. By using fluorescently labeled specific antigen binding fragments (Fabs), we have developed a general method to monitor the distribution and global level of endogenous histone H3 lysine modifications in living cells without disturbing cell growth and embryo development. Fabs produce distinct nuclear patterns that are characteristic of their target modifications. H3K27 trimethylation-specific Fabs, for example, are concentrated on inactive X chromosomes. As Fabs bind their targets transiently, the ratio of bound and free molecules depends on the target concentration, allowing us to measure changes in global modification levels. High-affinity Fabs are suitable for mouse embryo imaging, so we have used them to monitor H3K9 and H3K27 acetylation levels in mouse preimplantation embryos produced by in vitro fertilization and somatic cell nuclear transfer. The data suggest that a high level of H3K27 acetylation is important for normal embryo development. As Fab-based live endogenous modification labeling (FabLEM) is broadly useful for visualizing any modification, it should be a powerful tool for studying cell signaling and diagnosis in the future.

    Original languageEnglish
    Pages (from-to)6475-6488
    Number of pages14
    JournalNucleic Acids Research
    Volume39
    Issue number15
    DOIs
    Publication statusPublished - 2011 Aug

    Fingerprint

    Histone Code
    Epigenomics
    Acetylation
    Embryonic Development
    X Chromosome
    Blastocyst
    Fertilization in Vitro
    Growth and Development
    Histones
    Lysine
    Embryonic Structures
    Genome
    Antigens
    Genes

    ASJC Scopus subject areas

    • Genetics

    Cite this

    Hayashi-Takanaka, Y., Yamagata, K., Wakayama, T., Stasevich, T. J., Kainuma, T., Tsurimoto, T., ... Kimura, H. (2011). Tracking epigenetic histone modifications in single cells using Fab-based live endogenous modification labeling. Nucleic Acids Research, 39(15), 6475-6488. https://doi.org/10.1093/nar/gkr343

    Tracking epigenetic histone modifications in single cells using Fab-based live endogenous modification labeling. / Hayashi-Takanaka, Yoko; Yamagata, Kazuo; Wakayama, Teruhiko; Stasevich, Timothy J.; Kainuma, Takashi; Tsurimoto, Toshiki; Tachibana, Makoto; Shinkai, Yoichi; Kurumizaka, Hitoshi; Nozaki, Naohito; Kimura, Hiroshi.

    In: Nucleic Acids Research, Vol. 39, No. 15, 08.2011, p. 6475-6488.

    Research output: Contribution to journalArticle

    Hayashi-Takanaka, Y, Yamagata, K, Wakayama, T, Stasevich, TJ, Kainuma, T, Tsurimoto, T, Tachibana, M, Shinkai, Y, Kurumizaka, H, Nozaki, N & Kimura, H 2011, 'Tracking epigenetic histone modifications in single cells using Fab-based live endogenous modification labeling', Nucleic Acids Research, vol. 39, no. 15, pp. 6475-6488. https://doi.org/10.1093/nar/gkr343
    Hayashi-Takanaka Y, Yamagata K, Wakayama T, Stasevich TJ, Kainuma T, Tsurimoto T et al. Tracking epigenetic histone modifications in single cells using Fab-based live endogenous modification labeling. Nucleic Acids Research. 2011 Aug;39(15):6475-6488. https://doi.org/10.1093/nar/gkr343
    Hayashi-Takanaka, Yoko ; Yamagata, Kazuo ; Wakayama, Teruhiko ; Stasevich, Timothy J. ; Kainuma, Takashi ; Tsurimoto, Toshiki ; Tachibana, Makoto ; Shinkai, Yoichi ; Kurumizaka, Hitoshi ; Nozaki, Naohito ; Kimura, Hiroshi. / Tracking epigenetic histone modifications in single cells using Fab-based live endogenous modification labeling. In: Nucleic Acids Research. 2011 ; Vol. 39, No. 15. pp. 6475-6488.
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