TRAF-interacting protein with a forkhead-associated domain B (TIFAB) is a negative regulator of the TRAF6-induced cellular functions.

Takayuki Matsumura, Junko Kawamura-Tsuzuku, Tadashi Yamamoto, Kentaro Senba, Jun Ichiro Inoue

    Research output: Contribution to journalArticle

    7 Citations (Scopus)

    Abstract

    Tumour necrosis factor receptor-associated factor (TRAF)-interacting protein with a forkhead-associated domain (TIFA) activates TRAF6 to induce NF-kappaB activation. TIFA-related protein, TIFAB, is highly expressed in the spleen and inhibits TIFA-mediated TRAF6 activation. However, little is known about cell types that express TIFAB and its function in those cells. Here, we show that TIFAB is mainly expressed in B cells rather than T cells in the spleen and that the expression level was much higher in dendritic cells (DCs) and macrophages than that in splenic lymphocytes. TIFAB expression was downregulated when B cells, DCs or macrophages were stimulated by TRAF6-mediated proliferative or maturation signals including those emanating from CD40, sIgM and TLRs. Furthermore, microinjection experiments using NIH3T3 cells revealed that TIFAB inhibited entry into the S phase of the cell cycle. Our results suggest that TIFAB could act as a negative regulator of the TRAF6-induced cellular function such as B cell proliferation and maturation of DCs and macrophages.

    Original languageEnglish
    Pages (from-to)375-381
    Number of pages7
    JournalJournal of Biochemistry
    Volume146
    Issue number3
    Publication statusPublished - 2009 Sep

    Fingerprint

    TNF Receptor-Associated Factor 6
    Tumor Necrosis Factor Receptor-Associated Peptides and Proteins
    Macrophages
    Dendritic Cells
    B-Lymphocytes
    Proteins
    Spleen
    Chemical activation
    Cells
    T-cells
    Lymphocytes
    NF-kappa B
    Cell proliferation
    Microinjections
    S Phase
    Cell Cycle
    Down-Regulation
    Cell Proliferation
    T-Lymphocytes
    Experiments

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology

    Cite this

    TRAF-interacting protein with a forkhead-associated domain B (TIFAB) is a negative regulator of the TRAF6-induced cellular functions. / Matsumura, Takayuki; Kawamura-Tsuzuku, Junko; Yamamoto, Tadashi; Senba, Kentaro; Inoue, Jun Ichiro.

    In: Journal of Biochemistry, Vol. 146, No. 3, 09.2009, p. 375-381.

    Research output: Contribution to journalArticle

    Matsumura, Takayuki ; Kawamura-Tsuzuku, Junko ; Yamamoto, Tadashi ; Senba, Kentaro ; Inoue, Jun Ichiro. / TRAF-interacting protein with a forkhead-associated domain B (TIFAB) is a negative regulator of the TRAF6-induced cellular functions. In: Journal of Biochemistry. 2009 ; Vol. 146, No. 3. pp. 375-381.
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    AU - Kawamura-Tsuzuku, Junko

    AU - Yamamoto, Tadashi

    AU - Senba, Kentaro

    AU - Inoue, Jun Ichiro

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