Transcription Factors as Evolvable Biosensors

Daisuke Umeno, Yuki Kimura, Shigeko Kawai-Noma

Research output: Contribution to journalArticlepeer-review


One of the most prominent features of genetically encoded biosensors (GEBs) is their evolvability-the ability to invent new sensory functions using mutations. Among the GEBs, the transcription factor-based biosensors (TF-biosensors) is the focus of this review. We also discuss how this class of sensors can be highly evolvable and how we can exploit it. With an established platform for directed evolution, researchers can create, or evolve, new TF-biosensors. Directed evolution experiments have revealed the TF-biosensors' evolvability, which is based partially on their characteristic physicochemical properties.

Original languageEnglish
Pages (from-to)699-705
Number of pages7
JournalAnalytical Sciences
Issue number5
Publication statusPublished - 2021 May 10
Externally publishedYes


  • biosensors
  • Genetic switches

ASJC Scopus subject areas

  • Analytical Chemistry


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