Transcription Profiles of Endothelial Cells in the Rat Ductus Arteriosus during a Perinatal Period

Norika Mengchia Liu, Tomohiro Yokota, Shun Maekawa, Ping Lü, Inbun Tei, Hideki Taniguchi, Utako Yokoyama, Takashi Kato, Susumu Minamisawa

    Research output: Contribution to journalArticle

    8 Citations (Scopus)

    Abstract

    Endothelial cells (ECs) lining the blood vessels serve a variety of functions and play a central role in the homeostasis of the circulatory system. Since the ductus arteriosus (DA) has different arterial characteristics from its connecting vessels, we hypothesized that ECs of the DA exhibited a unique gene profile involved in the regulation of DA-specific morphology and function. Using a fluorescence-activated cell sorter, we isolated ECs from pooled tissues from the DA or the descending aorta of Wistar rat fetuses at full-term of gestation (F group) or neonates 30 minutes after birth (N group). Using anti-CD31 and anti-CD45 antibodies as cell surface markers for ECs and hematopoietic derived cells, respectively, cDNAs from the CD31-positive and CD45-negative cells were hybridized to the Affymetrix GeneChip® Rat Gene 1.0 ST Array. Among 26,469 gene-level probe sets, 82 genes in the F group and 81 genes in the N group were expressed at higher levels in DA ECs than in aortic ECs (p<0.05, fold change>2.0). In addition to well-known endothelium-enriched genes such as Tgfb2 and Vegfa, novel DA endothelium-dominant genes including Slc38a1, Capn6, and Lrat were discovered. Enrichment analysis using GeneGo MetaCore software showed that DA endothelium-related biological processes were involved in morphogenesis and development. We identified many overlapping genes in each process including neural crest-related genes (Hoxa1, Hoxa4, and Hand2, etc) and the second heart field-related genes (Tbx1, Isl1, and Fgf10, etc). Moreover, we found that regulation of epithelial-to-mesenchymal transition, cell adhesion, and retinol metabolism are the active pathways involved in the network via potential interactions with many of the identified genes to form DA-specific endothelia. In conclusion, the present study uncovered several significant differences of the transcriptional profile between the DA and aortic ECs. Newly identified DA endothelium-dominant genes may play an important role in DA-specific functional and morphologic characteristics.

    Original languageEnglish
    Article numbere73685
    JournalPLoS One
    Volume8
    Issue number9
    DOIs
    Publication statusPublished - 2013 Sep 27

    Fingerprint

    ductus arteriosus
    perinatal period
    Ductus Arteriosus
    Endothelial cells
    Transcription
    endothelial cells
    Rats
    Endothelial Cells
    transcription (genetics)
    Genes
    rats
    endothelium
    Endothelium
    genes
    Dominant Genes
    dominant genes
    Overlapping Genes
    cells
    Biological Phenomena
    circulatory system

    ASJC Scopus subject areas

    • Agricultural and Biological Sciences(all)
    • Biochemistry, Genetics and Molecular Biology(all)
    • Medicine(all)

    Cite this

    Liu, N. M., Yokota, T., Maekawa, S., Lü, P., Tei, I., Taniguchi, H., ... Minamisawa, S. (2013). Transcription Profiles of Endothelial Cells in the Rat Ductus Arteriosus during a Perinatal Period. PLoS One, 8(9), [e73685]. https://doi.org/10.1371/journal.pone.0073685

    Transcription Profiles of Endothelial Cells in the Rat Ductus Arteriosus during a Perinatal Period. / Liu, Norika Mengchia; Yokota, Tomohiro; Maekawa, Shun; Lü, Ping; Tei, Inbun; Taniguchi, Hideki; Yokoyama, Utako; Kato, Takashi; Minamisawa, Susumu.

    In: PLoS One, Vol. 8, No. 9, e73685, 27.09.2013.

    Research output: Contribution to journalArticle

    Liu, NM, Yokota, T, Maekawa, S, Lü, P, Tei, I, Taniguchi, H, Yokoyama, U, Kato, T & Minamisawa, S 2013, 'Transcription Profiles of Endothelial Cells in the Rat Ductus Arteriosus during a Perinatal Period', PLoS One, vol. 8, no. 9, e73685. https://doi.org/10.1371/journal.pone.0073685
    Liu, Norika Mengchia ; Yokota, Tomohiro ; Maekawa, Shun ; Lü, Ping ; Tei, Inbun ; Taniguchi, Hideki ; Yokoyama, Utako ; Kato, Takashi ; Minamisawa, Susumu. / Transcription Profiles of Endothelial Cells in the Rat Ductus Arteriosus during a Perinatal Period. In: PLoS One. 2013 ; Vol. 8, No. 9.
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