Transcriptional profiles in the chicken ductus arteriosus during hatching

Toru Akaike, Satoko Shinjo, Eriko Ohmori, Ichige Kajimura, Nobuhito Goda, Susumu Minamisawa

Research output: Contribution to journalArticle

Abstract

The ductus arteriosus, an essential embryonic blood vessel between the pulmonary artery and the descending aorta, constricts after birth or hatching and eventually closes to terminate embryonic circulation. Chicken embryos have two long ductus arteriosi, which anatomically differ from mammal ductus arteriosus. Each long ductus arteriosus is divided into two parts: the pulmonary artery-sided and descending aorta-sided ductus arteriosi. Although the pulmonary artery-sided and descending aorta-sided ductus arteriosi have distinct functional characteristics, such as oxygen responsiveness, the difference in their transcriptional profiles has not been investigated. We performed a DNA microarray analysis (GSE 120116 at NCBI GEO) with pooled tissues from the chicken pulmonary artery-sided ductus arteriosus, descending aorta-sided ductus arteriosus, and aorta at the internal pipping stage. Although several known ductus arteriosus-dominant genes such as tfap2b were highly expressed in the pulmonary artery-sided ductus arteriosus, we newly found genes that were dominantly expressed in the chicken pulmonary artery-sided ductus arteriosus. Interestingly, cluster analysis showed that the expression pattern of the pulmonary artery-sided ductus arteriosus was closer to that of the descending aorta-sided ductus arteriosus than that of the aorta, whereas the morphology of the descending aorta-sided ductus arteriosus was closer to that of the aorta than that of the pulmonary artery-sided ductus arteriosus. Subsequent pathway analysis with DAVID bioinformatics resources revealed that the pulmonary artery-sided ductus arteriosus showed enhanced expression of the genes involved in melanogenesis and tyrosine metabolism compared with the descending aorta-sided ductus arteriosus, suggesting that tyrosinase and the related genes play an important role in the proper differentiation of neural crest-derived cells during vascular remodeling in the ductus arteriosus. In conclusion, the transcription profiles of the chicken ductus arteriosus provide new insights for investigating the mechanism of ductus arteriosus closure.

Original languageEnglish
Article numbere0214139
JournalPloS one
Volume14
Issue number3
DOIs
Publication statusPublished - 2019 Mar 1

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ductus arteriosus
Ductus Arteriosus
Chickens
hatching
Genes
chickens
pulmonary artery
aorta
Pulmonary Artery
Thoracic Aorta
Mammals
Monophenol Monooxygenase
Blood vessels
Cluster analysis
Transcription
Bioinformatics
Microarrays
Metabolism
Tyrosine
Tissue

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Akaike, T., Shinjo, S., Ohmori, E., Kajimura, I., Goda, N., & Minamisawa, S. (2019). Transcriptional profiles in the chicken ductus arteriosus during hatching. PloS one, 14(3), [e0214139]. https://doi.org/10.1371/journal.pone.0214139

Transcriptional profiles in the chicken ductus arteriosus during hatching. / Akaike, Toru; Shinjo, Satoko; Ohmori, Eriko; Kajimura, Ichige; Goda, Nobuhito; Minamisawa, Susumu.

In: PloS one, Vol. 14, No. 3, e0214139, 01.03.2019.

Research output: Contribution to journalArticle

Akaike, T, Shinjo, S, Ohmori, E, Kajimura, I, Goda, N & Minamisawa, S 2019, 'Transcriptional profiles in the chicken ductus arteriosus during hatching' PloS one, vol. 14, no. 3, e0214139. https://doi.org/10.1371/journal.pone.0214139
Akaike, Toru ; Shinjo, Satoko ; Ohmori, Eriko ; Kajimura, Ichige ; Goda, Nobuhito ; Minamisawa, Susumu. / Transcriptional profiles in the chicken ductus arteriosus during hatching. In: PloS one. 2019 ; Vol. 14, No. 3.
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