Transcriptional profiling in mouse skeletal muscle following a single bout of voluntary running: Evidence of increased cell proliferation

Sangdun Choi, Xuebin Liu, Ping Li, Takayuki Akimoto, Sun Young Lee, Mei Zhang, Zhen Yan

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Skeletal muscle undergoes adaptation following repetitive bouts of exercise. We hypothesize that transcriptional reprogramming and cellular remodeling start in the early phase of long-term training and play an important role in skeletal muscle adaptation. The aim of this study was to define the global mRNA expression in mouse plantaris muscle during (run for 3 and 12 h) and after (3, 6, 12, and 24 h postexercise) a single bout of voluntary running and compare it with that after long-term training (4 wk of running). Among 15,832 gene elements surveyed in a high-density cDNA microarray analysis, 900 showed more than two-fold changes at one or more time points. K-means clustering and cumulative hypergeometric probability distribution analyses revealed a significant enrichment of genes involved in defense, cell cycle, cell adhesion and motility, signal transduction, and apoptosis, with induced expression patterns sharing similar patterns with that of peroxisome proliferator activator receptor-γ coactivator-1α and vascular endothelial growth factor A. We focused on the finding of a delayed (at 24 h postexercise) induction of mRNA expression of cell cycle genes origin recognition complex 1, cyclin A2, and cell division 2 homolog A (Schizoccharomyces pombe) and confirmed increased cell proliferation by in vivo 5-bromo-2′-deoxyuridine labeling following voluntary running. X-ray irradiation of the hindlimb significantly diminished exercise-induced 5-bromo-2′-deoxyuridine incorporation. These findings suggest that a single bout of voluntary running activates the transcriptional network and promotes adaptive processes in skeletal muscle, including cell proliferation.

Original languageEnglish
Pages (from-to)2406-2415
Number of pages10
JournalJournal of Applied Physiology
Volume99
Issue number6
DOIs
Publication statusPublished - 2005 Dec
Externally publishedYes

Fingerprint

Running
Skeletal Muscle
Cell Proliferation
Bromodeoxyuridine
Cyclin A2
Origin Recognition Complex
Peroxisome Proliferators
cdc Genes
Messenger RNA
Gene Regulatory Networks
Microarray Analysis
Hindlimb
Oligonucleotide Array Sequence Analysis
Cell Adhesion
Cell Division
Muscle Cells
Vascular Endothelial Growth Factor A
Genes
Cell Movement
Cluster Analysis

Keywords

  • Adaptation
  • Cellular remodeling
  • Exercise
  • High-density complementary deoxyribonucleic acid microarray
  • Transcriptional reprogramming

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Transcriptional profiling in mouse skeletal muscle following a single bout of voluntary running : Evidence of increased cell proliferation. / Choi, Sangdun; Liu, Xuebin; Li, Ping; Akimoto, Takayuki; Lee, Sun Young; Zhang, Mei; Yan, Zhen.

In: Journal of Applied Physiology, Vol. 99, No. 6, 12.2005, p. 2406-2415.

Research output: Contribution to journalArticle

Choi, Sangdun ; Liu, Xuebin ; Li, Ping ; Akimoto, Takayuki ; Lee, Sun Young ; Zhang, Mei ; Yan, Zhen. / Transcriptional profiling in mouse skeletal muscle following a single bout of voluntary running : Evidence of increased cell proliferation. In: Journal of Applied Physiology. 2005 ; Vol. 99, No. 6. pp. 2406-2415.
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