Transcriptional regulation of human β-galactoside α2,6-sialyltransferase (hST6Gal I) gene in colon adenocarcinoma cell line

Liming Xu; Yoshikazu Kurusu; Kaori Takizawa; Junzo Tanaka; Kojiro Matsumoto; Akiyoshi Taniguchi

doi:10.1016/S0006-291X(03)01314-7

Transcriptional regulation of human β-galactoside α2,6-sialyltransferase (hST6Gal I) gene in colon adenocarcinoma cell line

Liming Xu, Yoshikazu Kurusu, Kaori Takizawa, Junzo Tanaka, Kojiro Matsumoto, Akiyoshi Taniguchi

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

Previous studies have shown that hST6Gal I mRNA is overexpressed in colorectal cancer tissues compared with non-malignant or benign tissue. Moreover, Form 1 (hepatic form) mRNA isoform had a marked tendency to accumulate in colon cancer [Int. J. Cancer 88 (2000) 58-65]. These findings suggest that the transcriptional regulation of Form 1 is altered during malignant transformation. We report here transcriptional regulation of the hST6Gal I gene in colon adenocarcinoma cell lines. We characterized P1 promoter region, which regulates Form 1 mRNA expression, using luciferase assays. The result indicates that the nt-156 to -1 region is important for transcriptional activity of hST6Gal I gene in colon adenocarcinoma cell lines. The nt-156 to -1 region contains HNF1 recognition element. Mutation of the HNF1 site reduced luciferase activity by about 80% compared with the wild-type construct, suggesting that HNF1 site is involved in the transcription of Form 1 mRNA in colon cancer cells.

Original language	English
Pages (from-to)	1070-1074
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	307
Issue number	4
DOIs	https://doi.org/10.1016/S0006-291X(03)01314-7
Publication status	Published - 2003 Aug 8
Externally published	Yes

Keywords

Colon adenocarcinoma
DCoH
HNF-1
Sialyltransferase
Transcriptional regulation

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/S0006-291X(03)01314-7

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Transcriptional regulation of human β-galactoside α2,6-sialyltransferase (hST6Gal I) gene in colon adenocarcinoma cell line. / Xu, Liming; Kurusu, Yoshikazu; Takizawa, Kaori; Tanaka, Junzo; Matsumoto, Kojiro; Taniguchi, Akiyoshi.

In: Biochemical and Biophysical Research Communications, Vol. 307, No. 4, 08.08.2003, p. 1070-1074.

Research output: Contribution to journal › Article › peer-review

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title = "Transcriptional regulation of human β-galactoside α2,6-sialyltransferase (hST6Gal I) gene in colon adenocarcinoma cell line",

abstract = "Previous studies have shown that hST6Gal I mRNA is overexpressed in colorectal cancer tissues compared with non-malignant or benign tissue. Moreover, Form 1 (hepatic form) mRNA isoform had a marked tendency to accumulate in colon cancer [Int. J. Cancer 88 (2000) 58-65]. These findings suggest that the transcriptional regulation of Form 1 is altered during malignant transformation. We report here transcriptional regulation of the hST6Gal I gene in colon adenocarcinoma cell lines. We characterized P1 promoter region, which regulates Form 1 mRNA expression, using luciferase assays. The result indicates that the nt-156 to -1 region is important for transcriptional activity of hST6Gal I gene in colon adenocarcinoma cell lines. The nt-156 to -1 region contains HNF1 recognition element. Mutation of the HNF1 site reduced luciferase activity by about 80% compared with the wild-type construct, suggesting that HNF1 site is involved in the transcription of Form 1 mRNA in colon cancer cells.",

keywords = "Colon adenocarcinoma, DCoH, HNF-1, Sialyltransferase, Transcriptional regulation",

author = "Liming Xu and Yoshikazu Kurusu and Kaori Takizawa and Junzo Tanaka and Kojiro Matsumoto and Akiyoshi Taniguchi",

note = "Funding Information: We are grateful to Dr Fabio Dal{\textquoteright}Olio for stimulating discussions. This work was supported in part by Grant No. 14572076 from the Ministry of Education, Culture, Sports, Science and Technology, Japan.",

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doi = "10.1016/S0006-291X(03)01314-7",

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T1 - Transcriptional regulation of human β-galactoside α2,6-sialyltransferase (hST6Gal I) gene in colon adenocarcinoma cell line

AU - Xu, Liming

AU - Kurusu, Yoshikazu

AU - Takizawa, Kaori

AU - Tanaka, Junzo

AU - Matsumoto, Kojiro

AU - Taniguchi, Akiyoshi

N1 - Funding Information: We are grateful to Dr Fabio Dal’Olio for stimulating discussions. This work was supported in part by Grant No. 14572076 from the Ministry of Education, Culture, Sports, Science and Technology, Japan.

PY - 2003/8/8

Y1 - 2003/8/8

N2 - Previous studies have shown that hST6Gal I mRNA is overexpressed in colorectal cancer tissues compared with non-malignant or benign tissue. Moreover, Form 1 (hepatic form) mRNA isoform had a marked tendency to accumulate in colon cancer [Int. J. Cancer 88 (2000) 58-65]. These findings suggest that the transcriptional regulation of Form 1 is altered during malignant transformation. We report here transcriptional regulation of the hST6Gal I gene in colon adenocarcinoma cell lines. We characterized P1 promoter region, which regulates Form 1 mRNA expression, using luciferase assays. The result indicates that the nt-156 to -1 region is important for transcriptional activity of hST6Gal I gene in colon adenocarcinoma cell lines. The nt-156 to -1 region contains HNF1 recognition element. Mutation of the HNF1 site reduced luciferase activity by about 80% compared with the wild-type construct, suggesting that HNF1 site is involved in the transcription of Form 1 mRNA in colon cancer cells.

AB - Previous studies have shown that hST6Gal I mRNA is overexpressed in colorectal cancer tissues compared with non-malignant or benign tissue. Moreover, Form 1 (hepatic form) mRNA isoform had a marked tendency to accumulate in colon cancer [Int. J. Cancer 88 (2000) 58-65]. These findings suggest that the transcriptional regulation of Form 1 is altered during malignant transformation. We report here transcriptional regulation of the hST6Gal I gene in colon adenocarcinoma cell lines. We characterized P1 promoter region, which regulates Form 1 mRNA expression, using luciferase assays. The result indicates that the nt-156 to -1 region is important for transcriptional activity of hST6Gal I gene in colon adenocarcinoma cell lines. The nt-156 to -1 region contains HNF1 recognition element. Mutation of the HNF1 site reduced luciferase activity by about 80% compared with the wild-type construct, suggesting that HNF1 site is involved in the transcription of Form 1 mRNA in colon cancer cells.

KW - Colon adenocarcinoma

KW - DCoH

KW - HNF-1

KW - Sialyltransferase

KW - Transcriptional regulation

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Transcriptional regulation of human β-galactoside α2,6-sialyltransferase (hST6Gal I) gene in colon adenocarcinoma cell line

Abstract

Keywords

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