Transcriptional regulation of human β-galactoside α2,6-sialyltransferase (hST6Gal I) gene in colon adenocarcinoma cell line

Liming Xu, Yoshikazu Kurusu, Kaori Takizawa, Junzo Tanaka, Kojiro Matsumoto, Akiyoshi Taniguchi

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Previous studies have shown that hST6Gal I mRNA is overexpressed in colorectal cancer tissues compared with non-malignant or benign tissue. Moreover, Form 1 (hepatic form) mRNA isoform had a marked tendency to accumulate in colon cancer [Int. J. Cancer 88 (2000) 58-65]. These findings suggest that the transcriptional regulation of Form 1 is altered during malignant transformation. We report here transcriptional regulation of the hST6Gal I gene in colon adenocarcinoma cell lines. We characterized P1 promoter region, which regulates Form 1 mRNA expression, using luciferase assays. The result indicates that the nt-156 to -1 region is important for transcriptional activity of hST6Gal I gene in colon adenocarcinoma cell lines. The nt-156 to -1 region contains HNF1 recognition element. Mutation of the HNF1 site reduced luciferase activity by about 80% compared with the wild-type construct, suggesting that HNF1 site is involved in the transcription of Form 1 mRNA in colon cancer cells.

Original languageEnglish
Pages (from-to)1070-1074
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume307
Issue number4
DOIs
Publication statusPublished - 2003 Aug 8
Externally publishedYes

Fingerprint

Sialyltransferases
Galactosides
Colon
Adenocarcinoma
Genes
Cells
Luciferases
Cell Line
Colonic Neoplasms
Messenger RNA
Tissue
RNA Isoforms
Transcription
Genetic Promoter Regions
Colorectal Neoplasms
Assays
Mutation
Liver
beta-D-galactoside alpha 2-6-sialyltransferase
Neoplasms

Keywords

  • Colon adenocarcinoma
  • DCoH
  • HNF-1
  • Sialyltransferase
  • Transcriptional regulation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Transcriptional regulation of human β-galactoside α2,6-sialyltransferase (hST6Gal I) gene in colon adenocarcinoma cell line. / Xu, Liming; Kurusu, Yoshikazu; Takizawa, Kaori; Tanaka, Junzo; Matsumoto, Kojiro; Taniguchi, Akiyoshi.

In: Biochemical and Biophysical Research Communications, Vol. 307, No. 4, 08.08.2003, p. 1070-1074.

Research output: Contribution to journalArticle

Xu, Liming ; Kurusu, Yoshikazu ; Takizawa, Kaori ; Tanaka, Junzo ; Matsumoto, Kojiro ; Taniguchi, Akiyoshi. / Transcriptional regulation of human β-galactoside α2,6-sialyltransferase (hST6Gal I) gene in colon adenocarcinoma cell line. In: Biochemical and Biophysical Research Communications. 2003 ; Vol. 307, No. 4. pp. 1070-1074.
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AU - Tanaka, Junzo

AU - Matsumoto, Kojiro

AU - Taniguchi, Akiyoshi

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