Transcriptional regulation of human β-galactoside α2,6-sialyltransferase (hST6Gal I) gene during differentiation of the HL-60 cell line

Akiyoshi Taniguchi, Yuko Hasegawa, Koji Higai, Kojiro Matsumoto

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

We have previously shown that the expression of β-galactoside α2,6-sialyltransferase (hST6Gal I) mRNA decreases during HL-60 differentiation induced with dimethyl sulfoxide (DMSO) and that transcriptional regulation depends on the P3 promoter that exists S-upstream of exon Y. The regulation of hST6Gal I may be important for the expression of sialyl-Le(X) in HL-60 cells. In the present report, we studied the transcriptional regulation of hST6Gal I gene during DMSO-induced differentiation of HL-60 cells. To elucidate the molecular basis of hST6Gal I gene expression, the genomic region containing the P3 promoter of hST6Gal I was isolated and functionally characterized. Using a luciferase assay, we identified a functional DNA portion that confers an enhancer, located at nucleotide number (nt) -317 to -174 within the P3 promoter of hST6Gal I genomic DNA. This element contains two sequences similar to Sp1 (GC-box) and one sequence similar to Oct-1 recognition motifs (octamer sequence). Site-directed mutagenesis of Sp1 and Oct-1 sites showed that two Sp1 motifs and one Oct-1 motif are essential for transcriptional activity in HL-60 cells. Enhancer activity is suppressed during HL-60 cell differentiation induced with DMSO. These results suggest that GC-hox and octamer sequence may play a critical role in the transcriptional regulation of the hST6Gal I gene during HL-60 cell differentiation.

Original languageEnglish
Pages (from-to)623-628
Number of pages6
JournalGlycobiology
Volume10
Issue number6
Publication statusPublished - 2000 Jun
Externally publishedYes

Fingerprint

Sialyltransferases
Galactosides
HL-60 Cells
Genes
Cells
Cell Line
Dimethyl Sulfoxide
Cell Differentiation
Mutagenesis
DNA
Site-Directed Mutagenesis
beta-D-galactoside alpha 2-6-sialyltransferase
Luciferases
Gene expression
Exons
Assays
Nucleotides
Gene Expression
Messenger RNA

Keywords

  • Gene expression
  • HL-60
  • Sialyl-Le(X)
  • Sialyltransferase
  • Transcriptional regulation

ASJC Scopus subject areas

  • Biochemistry

Cite this

Transcriptional regulation of human β-galactoside α2,6-sialyltransferase (hST6Gal I) gene during differentiation of the HL-60 cell line. / Taniguchi, Akiyoshi; Hasegawa, Yuko; Higai, Koji; Matsumoto, Kojiro.

In: Glycobiology, Vol. 10, No. 6, 06.2000, p. 623-628.

Research output: Contribution to journalArticle

@article{8f2a2dce84af411b88114e7d39a3428c,
title = "Transcriptional regulation of human β-galactoside α2,6-sialyltransferase (hST6Gal I) gene during differentiation of the HL-60 cell line",
abstract = "We have previously shown that the expression of β-galactoside α2,6-sialyltransferase (hST6Gal I) mRNA decreases during HL-60 differentiation induced with dimethyl sulfoxide (DMSO) and that transcriptional regulation depends on the P3 promoter that exists S-upstream of exon Y. The regulation of hST6Gal I may be important for the expression of sialyl-Le(X) in HL-60 cells. In the present report, we studied the transcriptional regulation of hST6Gal I gene during DMSO-induced differentiation of HL-60 cells. To elucidate the molecular basis of hST6Gal I gene expression, the genomic region containing the P3 promoter of hST6Gal I was isolated and functionally characterized. Using a luciferase assay, we identified a functional DNA portion that confers an enhancer, located at nucleotide number (nt) -317 to -174 within the P3 promoter of hST6Gal I genomic DNA. This element contains two sequences similar to Sp1 (GC-box) and one sequence similar to Oct-1 recognition motifs (octamer sequence). Site-directed mutagenesis of Sp1 and Oct-1 sites showed that two Sp1 motifs and one Oct-1 motif are essential for transcriptional activity in HL-60 cells. Enhancer activity is suppressed during HL-60 cell differentiation induced with DMSO. These results suggest that GC-hox and octamer sequence may play a critical role in the transcriptional regulation of the hST6Gal I gene during HL-60 cell differentiation.",
keywords = "Gene expression, HL-60, Sialyl-Le(X), Sialyltransferase, Transcriptional regulation",
author = "Akiyoshi Taniguchi and Yuko Hasegawa and Koji Higai and Kojiro Matsumoto",
year = "2000",
month = "6",
language = "English",
volume = "10",
pages = "623--628",
journal = "Glycobiology",
issn = "0959-6658",
publisher = "Oxford University Press",
number = "6",

}

TY - JOUR

T1 - Transcriptional regulation of human β-galactoside α2,6-sialyltransferase (hST6Gal I) gene during differentiation of the HL-60 cell line

AU - Taniguchi, Akiyoshi

AU - Hasegawa, Yuko

AU - Higai, Koji

AU - Matsumoto, Kojiro

PY - 2000/6

Y1 - 2000/6

N2 - We have previously shown that the expression of β-galactoside α2,6-sialyltransferase (hST6Gal I) mRNA decreases during HL-60 differentiation induced with dimethyl sulfoxide (DMSO) and that transcriptional regulation depends on the P3 promoter that exists S-upstream of exon Y. The regulation of hST6Gal I may be important for the expression of sialyl-Le(X) in HL-60 cells. In the present report, we studied the transcriptional regulation of hST6Gal I gene during DMSO-induced differentiation of HL-60 cells. To elucidate the molecular basis of hST6Gal I gene expression, the genomic region containing the P3 promoter of hST6Gal I was isolated and functionally characterized. Using a luciferase assay, we identified a functional DNA portion that confers an enhancer, located at nucleotide number (nt) -317 to -174 within the P3 promoter of hST6Gal I genomic DNA. This element contains two sequences similar to Sp1 (GC-box) and one sequence similar to Oct-1 recognition motifs (octamer sequence). Site-directed mutagenesis of Sp1 and Oct-1 sites showed that two Sp1 motifs and one Oct-1 motif are essential for transcriptional activity in HL-60 cells. Enhancer activity is suppressed during HL-60 cell differentiation induced with DMSO. These results suggest that GC-hox and octamer sequence may play a critical role in the transcriptional regulation of the hST6Gal I gene during HL-60 cell differentiation.

AB - We have previously shown that the expression of β-galactoside α2,6-sialyltransferase (hST6Gal I) mRNA decreases during HL-60 differentiation induced with dimethyl sulfoxide (DMSO) and that transcriptional regulation depends on the P3 promoter that exists S-upstream of exon Y. The regulation of hST6Gal I may be important for the expression of sialyl-Le(X) in HL-60 cells. In the present report, we studied the transcriptional regulation of hST6Gal I gene during DMSO-induced differentiation of HL-60 cells. To elucidate the molecular basis of hST6Gal I gene expression, the genomic region containing the P3 promoter of hST6Gal I was isolated and functionally characterized. Using a luciferase assay, we identified a functional DNA portion that confers an enhancer, located at nucleotide number (nt) -317 to -174 within the P3 promoter of hST6Gal I genomic DNA. This element contains two sequences similar to Sp1 (GC-box) and one sequence similar to Oct-1 recognition motifs (octamer sequence). Site-directed mutagenesis of Sp1 and Oct-1 sites showed that two Sp1 motifs and one Oct-1 motif are essential for transcriptional activity in HL-60 cells. Enhancer activity is suppressed during HL-60 cell differentiation induced with DMSO. These results suggest that GC-hox and octamer sequence may play a critical role in the transcriptional regulation of the hST6Gal I gene during HL-60 cell differentiation.

KW - Gene expression

KW - HL-60

KW - Sialyl-Le(X)

KW - Sialyltransferase

KW - Transcriptional regulation

UR - http://www.scopus.com/inward/record.url?scp=0034124195&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034124195&partnerID=8YFLogxK

M3 - Article

VL - 10

SP - 623

EP - 628

JO - Glycobiology

JF - Glycobiology

SN - 0959-6658

IS - 6

ER -