Transcriptional regulation of human Galβ1,3GalNAc/Galβ1, 4GlcNAc α2,3-sialyltransferase (hST3Gal IV) gene in testis and ovary cell lines

Akiyoshi Taniguchi, Maiko Hioki, Kojiro Matsumoto

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The mRNA expression of sialyltransferase genes is regulated in a cell-type-specific manner. The mRNAs of human Galβ1, 3GalNAc/Galβ1, 4 GlcNAc α2,3-sialyltransferase gene (hST3Gal IV) consist of six isoforms, type A1, A2, B1, B2, B3, and BX. These mRNAs are transcribed from different promoters, pA, pB1, pB2, pB3, and pBX, respectively. Type B mRNAs are expressed in several cells, whereas type A mRNAs are specifically expressed in testis, ovary, and placenta, suggesting that pA promoter activity is especially high in these tissues. We show herein germ-cell specific transcriptional regulation of the hST3Gal IV pA promoter. Using a luciferase assay, pA promoter activity is shown to be high in testis and ovary cell lines. We identified the enhancer region of the pA promoter, located at nt -520 to -420. These results suggest that this element plays a critical role in germ-cell specific regulation of the pA promoter. The results of site-directed mutagenesis suggest that AP2 and c-Ets sites in this region are involved in pA promoter activity, which in turn suggests that the hST3Gal IV gene is regulated in a tissue-restricted fashion at the level of transcription.

Original languageEnglish
Pages (from-to)764-768
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume301
Issue number3
DOIs
Publication statusPublished - 2003 Feb 14
Externally publishedYes

Keywords

  • Gene expression
  • Luciferase assay
  • Multiple promoter
  • Sialic acid
  • Sialyltransferase

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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