TY - JOUR
T1 - Transcriptional regulation of human Galβ1,3GalNAc/Galβ1, 4GlcNAc α2,3-sialyltransferase (hST3Gal IV) gene in testis and ovary cell lines
AU - Taniguchi, Akiyoshi
AU - Hioki, Maiko
AU - Matsumoto, Kojiro
N1 - Funding Information:
This work was supported in part by Grant No. 14572076 from the Ministry of Education, Culture, Sports, Science and Technology of Japan.
PY - 2003/2/14
Y1 - 2003/2/14
N2 - The mRNA expression of sialyltransferase genes is regulated in a cell-type-specific manner. The mRNAs of human Galβ1, 3GalNAc/Galβ1, 4 GlcNAc α2,3-sialyltransferase gene (hST3Gal IV) consist of six isoforms, type A1, A2, B1, B2, B3, and BX. These mRNAs are transcribed from different promoters, pA, pB1, pB2, pB3, and pBX, respectively. Type B mRNAs are expressed in several cells, whereas type A mRNAs are specifically expressed in testis, ovary, and placenta, suggesting that pA promoter activity is especially high in these tissues. We show herein germ-cell specific transcriptional regulation of the hST3Gal IV pA promoter. Using a luciferase assay, pA promoter activity is shown to be high in testis and ovary cell lines. We identified the enhancer region of the pA promoter, located at nt -520 to -420. These results suggest that this element plays a critical role in germ-cell specific regulation of the pA promoter. The results of site-directed mutagenesis suggest that AP2 and c-Ets sites in this region are involved in pA promoter activity, which in turn suggests that the hST3Gal IV gene is regulated in a tissue-restricted fashion at the level of transcription.
AB - The mRNA expression of sialyltransferase genes is regulated in a cell-type-specific manner. The mRNAs of human Galβ1, 3GalNAc/Galβ1, 4 GlcNAc α2,3-sialyltransferase gene (hST3Gal IV) consist of six isoforms, type A1, A2, B1, B2, B3, and BX. These mRNAs are transcribed from different promoters, pA, pB1, pB2, pB3, and pBX, respectively. Type B mRNAs are expressed in several cells, whereas type A mRNAs are specifically expressed in testis, ovary, and placenta, suggesting that pA promoter activity is especially high in these tissues. We show herein germ-cell specific transcriptional regulation of the hST3Gal IV pA promoter. Using a luciferase assay, pA promoter activity is shown to be high in testis and ovary cell lines. We identified the enhancer region of the pA promoter, located at nt -520 to -420. These results suggest that this element plays a critical role in germ-cell specific regulation of the pA promoter. The results of site-directed mutagenesis suggest that AP2 and c-Ets sites in this region are involved in pA promoter activity, which in turn suggests that the hST3Gal IV gene is regulated in a tissue-restricted fashion at the level of transcription.
KW - Gene expression
KW - Luciferase assay
KW - Multiple promoter
KW - Sialic acid
KW - Sialyltransferase
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U2 - 10.1016/S0006-291X(03)00041-X
DO - 10.1016/S0006-291X(03)00041-X
M3 - Article
C2 - 12565846
AN - SCOPUS:0037436228
SN - 0006-291X
VL - 301
SP - 764
EP - 768
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -