Transfection microarrays for high-throughput phenotypic screening of genes involved in cell migration.

Reiko Onuki-Nagasaki, Akira Nagasaki, Kazumi Hakamada, Taro Q.P. Uyeda, Satoshi Fujita, Masato Miyake, Jun Miyake

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Cell migration is important in several biological phenomena, such as cancer metastasis. Therefore, the identification of genes involved in cell migration might facilitate the discovery of antimetastatic drugs. However, screening of genes by the current methods can be complicated by factors related to cell stimulation, for example, abolition of contact inhibition and the release inflammatory cytokines from wounded cells during examinations of wound healing in vitro. To overcome these problems and identify genes involved in cell migration, in this chapter we describe the use of transfection microarrays for high-throughput phenotypic screening.

Original languageEnglish
Pages (from-to)193-203
Number of pages11
JournalMethods in molecular biology (Clifton, N.J.)
Volume629
DOIs
Publication statusPublished - 2010
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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