Transient expression of tobacco BBF1-related Dof proteins, BBF2 and BBF3, upregulates genes involved in virus resistance and pathogen defense

Nobumitsu Sasaki, Masamichi Matsumaru, Shota Odaira, Atsumi Nakata, Keiko Nakata, Ippei Nakayama, Koya Yamaguchi, Hiroshi Nyunoya

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Dof proteins are known as plant specific transcription factors involved in many physiological processes. Although tobacco species are likely to have multiple Dof proteins (termed as BBF), only BBF1 has been characterized. In this study, we cloned cDNAs of BBF2 and BBF3, which were assumed to be BBF1 homologues, and investigated biochemical and biological functions of BBF2 and BBF3. In gel shift assays, similarly to BBF1, recombinant BBF2 and BBF3 were shown to bind preferentially to DNA with a TAAAG Dof-binding core sequence. Fluorescently labeled BBF2 and BBF3, which were expressed transiently in tobacco epidermal cells, were shown to localize at the nucleus. In addition, as a result of quantitative gene expression analysis, we found that transient expression of each of the three BBF proteins enhanced the expression levels of the virus resistance gene N and multiple defense related genes. Based on these results, we discuss the possibility that BBF2 and BBF3 as well as BBF1 may play roles as transcription factors in the regulation of genes involved in defense responses.

Original languageEnglish
Pages (from-to)70-77
Number of pages8
JournalPhysiological and Molecular Plant Pathology
Volume89
DOIs
Publication statusPublished - 2015 Jan 1
Externally publishedYes

Fingerprint

Tobacco
tobacco
Up-Regulation
Viruses
viruses
pathogens
Transcription Factors
transcription factors
Physiological Phenomena
Genes
Proteins
genes
proteins
Complementary DNA
Gels
gels
Gene Expression
gene expression
DNA
assays

Keywords

  • AAAG motif
  • Defense response
  • Dof transcription factor
  • N resistance gene
  • Tobacco BBF protein

ASJC Scopus subject areas

  • Plant Science
  • Genetics

Cite this

Transient expression of tobacco BBF1-related Dof proteins, BBF2 and BBF3, upregulates genes involved in virus resistance and pathogen defense. / Sasaki, Nobumitsu; Matsumaru, Masamichi; Odaira, Shota; Nakata, Atsumi; Nakata, Keiko; Nakayama, Ippei; Yamaguchi, Koya; Nyunoya, Hiroshi.

In: Physiological and Molecular Plant Pathology, Vol. 89, 01.01.2015, p. 70-77.

Research output: Contribution to journalArticle

Sasaki, Nobumitsu ; Matsumaru, Masamichi ; Odaira, Shota ; Nakata, Atsumi ; Nakata, Keiko ; Nakayama, Ippei ; Yamaguchi, Koya ; Nyunoya, Hiroshi. / Transient expression of tobacco BBF1-related Dof proteins, BBF2 and BBF3, upregulates genes involved in virus resistance and pathogen defense. In: Physiological and Molecular Plant Pathology. 2015 ; Vol. 89. pp. 70-77.
@article{c29bc06f80c54768b415778aacc527b1,
title = "Transient expression of tobacco BBF1-related Dof proteins, BBF2 and BBF3, upregulates genes involved in virus resistance and pathogen defense",
abstract = "Dof proteins are known as plant specific transcription factors involved in many physiological processes. Although tobacco species are likely to have multiple Dof proteins (termed as BBF), only BBF1 has been characterized. In this study, we cloned cDNAs of BBF2 and BBF3, which were assumed to be BBF1 homologues, and investigated biochemical and biological functions of BBF2 and BBF3. In gel shift assays, similarly to BBF1, recombinant BBF2 and BBF3 were shown to bind preferentially to DNA with a TAAAG Dof-binding core sequence. Fluorescently labeled BBF2 and BBF3, which were expressed transiently in tobacco epidermal cells, were shown to localize at the nucleus. In addition, as a result of quantitative gene expression analysis, we found that transient expression of each of the three BBF proteins enhanced the expression levels of the virus resistance gene N and multiple defense related genes. Based on these results, we discuss the possibility that BBF2 and BBF3 as well as BBF1 may play roles as transcription factors in the regulation of genes involved in defense responses.",
keywords = "AAAG motif, Defense response, Dof transcription factor, N resistance gene, Tobacco BBF protein",
author = "Nobumitsu Sasaki and Masamichi Matsumaru and Shota Odaira and Atsumi Nakata and Keiko Nakata and Ippei Nakayama and Koya Yamaguchi and Hiroshi Nyunoya",
year = "2015",
month = "1",
day = "1",
doi = "10.1016/j.pmpp.2014.12.005",
language = "English",
volume = "89",
pages = "70--77",
journal = "Physiological and Molecular Plant Pathology",
issn = "0885-5765",
publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - Transient expression of tobacco BBF1-related Dof proteins, BBF2 and BBF3, upregulates genes involved in virus resistance and pathogen defense

AU - Sasaki, Nobumitsu

AU - Matsumaru, Masamichi

AU - Odaira, Shota

AU - Nakata, Atsumi

AU - Nakata, Keiko

AU - Nakayama, Ippei

AU - Yamaguchi, Koya

AU - Nyunoya, Hiroshi

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Dof proteins are known as plant specific transcription factors involved in many physiological processes. Although tobacco species are likely to have multiple Dof proteins (termed as BBF), only BBF1 has been characterized. In this study, we cloned cDNAs of BBF2 and BBF3, which were assumed to be BBF1 homologues, and investigated biochemical and biological functions of BBF2 and BBF3. In gel shift assays, similarly to BBF1, recombinant BBF2 and BBF3 were shown to bind preferentially to DNA with a TAAAG Dof-binding core sequence. Fluorescently labeled BBF2 and BBF3, which were expressed transiently in tobacco epidermal cells, were shown to localize at the nucleus. In addition, as a result of quantitative gene expression analysis, we found that transient expression of each of the three BBF proteins enhanced the expression levels of the virus resistance gene N and multiple defense related genes. Based on these results, we discuss the possibility that BBF2 and BBF3 as well as BBF1 may play roles as transcription factors in the regulation of genes involved in defense responses.

AB - Dof proteins are known as plant specific transcription factors involved in many physiological processes. Although tobacco species are likely to have multiple Dof proteins (termed as BBF), only BBF1 has been characterized. In this study, we cloned cDNAs of BBF2 and BBF3, which were assumed to be BBF1 homologues, and investigated biochemical and biological functions of BBF2 and BBF3. In gel shift assays, similarly to BBF1, recombinant BBF2 and BBF3 were shown to bind preferentially to DNA with a TAAAG Dof-binding core sequence. Fluorescently labeled BBF2 and BBF3, which were expressed transiently in tobacco epidermal cells, were shown to localize at the nucleus. In addition, as a result of quantitative gene expression analysis, we found that transient expression of each of the three BBF proteins enhanced the expression levels of the virus resistance gene N and multiple defense related genes. Based on these results, we discuss the possibility that BBF2 and BBF3 as well as BBF1 may play roles as transcription factors in the regulation of genes involved in defense responses.

KW - AAAG motif

KW - Defense response

KW - Dof transcription factor

KW - N resistance gene

KW - Tobacco BBF protein

UR - http://www.scopus.com/inward/record.url?scp=84921491496&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84921491496&partnerID=8YFLogxK

U2 - 10.1016/j.pmpp.2014.12.005

DO - 10.1016/j.pmpp.2014.12.005

M3 - Article

VL - 89

SP - 70

EP - 77

JO - Physiological and Molecular Plant Pathology

JF - Physiological and Molecular Plant Pathology

SN - 0885-5765

ER -