Transition of β-actinin isoforms during development of chicken skeletal muscle

Yoko Asami, Takashi Funatsu, Shin'ichi Ishiwata

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    We examined by means of the immunoblotting technique the transition of β-actinin isoforms during the development of the chicken from 5 day embryo to adult. As an antigen, β-actinin was prepared from adult chicken breast muscle (pectoralis major) and polyclonal antibody was obtained by injecting undenatured β-actinin into a rabbit. Immunoblotting examination of breast muscle at several stages of development (except 5 day embryo, in which the whole body minus the head and limbs was examined) showed that the species of β-actinin subunits change during development: 1) βI is already present in 5 day embryo, whereas βII appears only after 9 days. 2) In 5 day embryo, we found, instead of βII, a new subunit (designated βIII) that cross-reacts with the antibody, has the apparent molecular weight of 30,000 daltons and has a slightly alkaline isoelectric point compared with βI. The content of βIII gradually decreased and βIII completely disappeared a week after hatching. Such a type of transition of the isoforms in β-actinin subunits is similar to that observed in other muscle proteins. The transition of β-actinin isoforms may correlate to the organization of an I-Z-I brush, especially to the length determination of thin filaments, because the developmental stage at which βIII disappears coincides with that at which the length of thin filaments is strictly determined.

    Original languageEnglish
    Pages (from-to)72-75
    Number of pages4
    JournalJournal of Biochemistry
    Volume103
    Issue number1
    Publication statusPublished - 1988 Jan

    Fingerprint

    Actinin
    Skeletal muscle
    Embryo
    Muscle
    Chickens
    Protein Isoforms
    Skeletal Muscle
    Antibodies
    Filament
    Embryonic Structures
    Antibody
    Brushes
    Antigens
    Immunoblotting
    Breast
    Rabbit
    Molecular weight
    Correlate
    Pectoralis Muscles
    Proteins

    ASJC Scopus subject areas

    • Statistics, Probability and Uncertainty
    • Applied Mathematics
    • Physiology (medical)
    • Radiology Nuclear Medicine and imaging
    • Molecular Biology
    • Biochemistry

    Cite this

    Transition of β-actinin isoforms during development of chicken skeletal muscle. / Asami, Yoko; Funatsu, Takashi; Ishiwata, Shin'ichi.

    In: Journal of Biochemistry, Vol. 103, No. 1, 01.1988, p. 72-75.

    Research output: Contribution to journalArticle

    Asami, Y, Funatsu, T & Ishiwata, S 1988, 'Transition of β-actinin isoforms during development of chicken skeletal muscle', Journal of Biochemistry, vol. 103, no. 1, pp. 72-75.
    Asami, Yoko ; Funatsu, Takashi ; Ishiwata, Shin'ichi. / Transition of β-actinin isoforms during development of chicken skeletal muscle. In: Journal of Biochemistry. 1988 ; Vol. 103, No. 1. pp. 72-75.
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