Transition of bacterial spatial organization in a biofilm monitored by FISH and subsequent image analysis

Yoshiteru Aoi, S. Tsuneda, A. Hirata

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The dynamic transition of bacterial community structure in a biofilm was monitored by the fluorescence in situ hybridization (FISH) technique and subsequent image analysis. Heterotrophic bacteria that had occupied the outer layer were gradually decreased whereas ammonia-oxidizing bacteria (AOB) gradually increased their growth activity and extended their existence area to the outer layer of the biofilm through the gradual reduction of the C/N ratio. The spatial organization of AOB in the biofilm dynamically changed responding to the environmental conditions such as pH fluctuation and lack of dissolved oxygen (DO) and had great influence on the nitrification activity. The accumulation of nitrite was observed at lower DO concentration, which might be due to the property that nitrite-oxidizing bacteria (NOB) possess of higher Km values for oxygen than AOB.

Original languageEnglish
Pages (from-to)365-370
Number of pages6
JournalWater Science and Technology
Volume49
Issue number11-12
DOIs
Publication statusPublished - 2004

Keywords

  • Ammonia-oxidizing bacteria (AOB)
  • Bacterial community structure
  • Dynamic transition
  • Fluorescence in situ hybridization (FISH)
  • Nitrifying biofilm
  • Nitrite-oxidizing bacteria (NOB)

ASJC Scopus subject areas

  • Environmental Engineering
  • Water Science and Technology

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