Transition of bacterial spatial organization in a biofilm monitored by FISH and subsequent image analysis

Yoshiteru Aoi, Satoshi Tsuneda, A. Hirata

    Research output: Contribution to journalArticle

    8 Citations (Scopus)

    Abstract

    The dynamic transition of bacterial community structure in a biofilm was monitored by the fluorescence in situ hybridization (FISH) technique and subsequent image analysis. Heterotrophic bacteria that had occupied the outer layer were gradually decreased whereas ammonia-oxidizing bacteria (AOB) gradually increased their growth activity and extended their existence area to the outer layer of the biofilm through the gradual reduction of the C/N ratio. The spatial organization of AOB in the biofilm dynamically changed responding to the environmental conditions such as pH fluctuation and lack of dissolved oxygen (DO) and had great influence on the nitrification activity. The accumulation of nitrite was observed at lower DO concentration, which might be due to the property that nitrite-oxidizing bacteria (NOB) possess of higher Km values for oxygen than AOB.

    Original languageEnglish
    Pages (from-to)365-370
    Number of pages6
    JournalWater Science and Technology
    Volume49
    Issue number11-12
    Publication statusPublished - 2004

    Fingerprint

    Biofilms
    image analysis
    Image analysis
    biofilm
    Bacteria
    fluorescence
    Fluorescence
    bacterium
    Ammonia
    ammonia
    Dissolved oxygen
    nitrite
    dissolved oxygen
    Nitrification
    nitrification
    community structure
    environmental conditions
    in situ
    oxygen
    Oxygen

    Keywords

    • Ammonia-oxidizing bacteria (AOB)
    • Bacterial community structure
    • Dynamic transition
    • Fluorescence in situ hybridization (FISH)
    • Nitrifying biofilm
    • Nitrite-oxidizing bacteria (NOB)

    ASJC Scopus subject areas

    • Water Science and Technology

    Cite this

    Transition of bacterial spatial organization in a biofilm monitored by FISH and subsequent image analysis. / Aoi, Yoshiteru; Tsuneda, Satoshi; Hirata, A.

    In: Water Science and Technology, Vol. 49, No. 11-12, 2004, p. 365-370.

    Research output: Contribution to journalArticle

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