Transitions in oral and intestinal microflora composition and innate immune receptor-dependent stimulation during mouse development

Mizuho Hasegawa, Toshifumi Osaka, Kazuki Tawaratsumida, Takashi Yamazaki, Hiroyuki Tada, Grace Y. Chen, Satoshi Tsuneda, Gabriel Núñez, Naohiro Inohara

    Research output: Contribution to journalArticle

    37 Citations (Scopus)

    Abstract

    Commensal bacteria possess immunostimulatory activities that can modulate host responses to affect development and homeostasis in the intestine. However, how different populations of resident bacteria stimulate the immune system remains largely unknown. We characterized here the ability of intestinal and oral microflora to stimulate individual pattern recognition receptors (PRRs) in bone marrow-derived macrophages and mesothelial cells. The intestinal but not oral microflora elicited age- and cell type-specific immunostimulation. The immunostimulatory activity of the intestinal microflora varied among individual mice but was largely mediated via Toll-like receptor 4 (TLR4) during breast-feeding, whereas it became TLR4 independent after weaning. This transition was associated with a change from a microflora rich in TLR4-stimulatory proteobacteria to one dominated by Bacteroidales and/or Clostridiales that poorly stimulate TLR4. The major stimulatory activity of the intestinal microflora was still intact in NOD1-, NOD2-, TLR2-, TLR4-, TLR5-, TLR9-, TLR11-, ASC-, or RICK-deficient cells but still relied on the adaptor MyD88. These studies demonstrate a transition in the intestinal microflora accompanied by a dynamic change of its ability to stimulate different PRRs which control intestinalE homeostasis.

    Original languageEnglish
    Pages (from-to)639-650
    Number of pages12
    JournalInfection and Immunity
    Volume78
    Issue number2
    DOIs
    Publication statusPublished - 2010 Feb

    Fingerprint

    Toll-Like Receptor 4
    Pattern Recognition Receptors
    Aptitude
    Homeostasis
    Bacteria
    Proteobacteria
    Weaning
    Breast Feeding
    Intestines
    Immune System
    Immunization
    Macrophages
    Gastrointestinal Microbiome
    Population

    ASJC Scopus subject areas

    • Immunology
    • Microbiology
    • Parasitology
    • Infectious Diseases

    Cite this

    Transitions in oral and intestinal microflora composition and innate immune receptor-dependent stimulation during mouse development. / Hasegawa, Mizuho; Osaka, Toshifumi; Tawaratsumida, Kazuki; Yamazaki, Takashi; Tada, Hiroyuki; Chen, Grace Y.; Tsuneda, Satoshi; Núñez, Gabriel; Inohara, Naohiro.

    In: Infection and Immunity, Vol. 78, No. 2, 02.2010, p. 639-650.

    Research output: Contribution to journalArticle

    Hasegawa, M, Osaka, T, Tawaratsumida, K, Yamazaki, T, Tada, H, Chen, GY, Tsuneda, S, Núñez, G & Inohara, N 2010, 'Transitions in oral and intestinal microflora composition and innate immune receptor-dependent stimulation during mouse development', Infection and Immunity, vol. 78, no. 2, pp. 639-650. https://doi.org/10.1128/IAI.01043-09
    Hasegawa, Mizuho ; Osaka, Toshifumi ; Tawaratsumida, Kazuki ; Yamazaki, Takashi ; Tada, Hiroyuki ; Chen, Grace Y. ; Tsuneda, Satoshi ; Núñez, Gabriel ; Inohara, Naohiro. / Transitions in oral and intestinal microflora composition and innate immune receptor-dependent stimulation during mouse development. In: Infection and Immunity. 2010 ; Vol. 78, No. 2. pp. 639-650.
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