Trapping of biological macromolecules in the three-dimensional mesocage pore cavities of monolith adsorbents

M. A. Shenashen, Sherif A. El-Safty, M. Khairy

    Research output: Contribution to journalArticle

    22 Citations (Scopus)

    Abstract

    Gene technology is experiencing remarkable progress, and proteins are becoming crucial in the field of disease diagnosis and treatment. Adsorption of biomolecules on the surface of inorganic materials is an important technique for diagnostic assays and gene applications. In this study, highly ordered mesocage cubic Pm3n aluminumsilica monoliths were fabricated by the one-pot direct-templating of a microemulsion of the liquid crystalline phases of a Brij 56 surfactant. Mesocage cubic Pm3n aluminosilica monoliths with well-defined mesostructures offer high adsorption and loading capacity of proteins from an aqueous solution. Three-dimensional monoliths characterized by spherical pore cavities can potentially perform efficient adsorption and trapping of insulin, cytochrome C, lysozyme, myoglobin, β-lactoglobin proteins. A wide variety of characterization techniques such as SAXS, SEM, TEM, the Brunauer-Emmett- Teller method for nitrogen adsorption and surface area measurements, and TEM were used. The adsorption of proteins as well as the kinetic and thermodynamic characteristics of adsorption was studied, and adsorption isotherms were described by the Langmuir equation. Our findings indicated that monolayer coverage of proteins formed on mesoporous adsorbent surfaces during immobilization and uptake assays. Adsorption efficiency of proteins was attained after a number of reuse cycles, which indicates the presence of mesoporous adsorbents of biomolecules. Integration of mesoporous adsorbents may be feasible in various scientific fields such as nanobioscience, material science, artificial implantation, protein purification, biosensors, drug delivery systems, and molecular biology/biotechnology.

    Original languageEnglish
    Pages (from-to)679-692
    Number of pages14
    JournalJournal of Porous Materials
    Volume20
    Issue number4
    DOIs
    Publication statusPublished - 2013 Aug

    Fingerprint

    Macromolecules
    Adsorbents
    Proteins
    Adsorption
    Biomolecules
    Assays
    Cetomacrogol
    Genes
    Transmission electron microscopy
    Molecular biology
    Myoglobin
    Microemulsions
    Materials science
    Cytochromes
    Biotechnology
    Muramidase
    Adsorption isotherms
    Insulin
    Biosensors
    Surface-Active Agents

    Keywords

    • Adsorption
    • Mesocage cavities
    • Monoliths
    • Protein
    • Three-dimensional aluminosilica
    • Trapping

    ASJC Scopus subject areas

    • Mechanical Engineering
    • Mechanics of Materials
    • Materials Science(all)

    Cite this

    Trapping of biological macromolecules in the three-dimensional mesocage pore cavities of monolith adsorbents. / Shenashen, M. A.; El-Safty, Sherif A.; Khairy, M.

    In: Journal of Porous Materials, Vol. 20, No. 4, 08.2013, p. 679-692.

    Research output: Contribution to journalArticle

    Shenashen, M. A. ; El-Safty, Sherif A. ; Khairy, M. / Trapping of biological macromolecules in the three-dimensional mesocage pore cavities of monolith adsorbents. In: Journal of Porous Materials. 2013 ; Vol. 20, No. 4. pp. 679-692.
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