Triggering of high-speed neurite outgrowth using an optical microheater

Kotaro Oyama, Vadim Zeeb, Yuki Kawamura, Tomomi Arai, Mizuho Gotoh, Hideki Itoh, Takeshi Itabashi, Madoka Suzuki, Shin'Ichi Ishiwata

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Optical microheating is a powerful non-invasive method for manipulating biological functions such as gene expression, muscle contraction, and cell excitation. Here, we demonstrate its potential usage for regulating neurite outgrowth. We found that optical microheating with a water-absorbable 1,455-nm laser beam triggers directional and explosive neurite outgrowth and branching in rat hippocampal neurons. The focused laser beam under a microscope rapidly increases the local temperature from 36 °C to 41 °C (stabilized within 2 s), resulting in the elongation of neurites by more than 10 1/4m within 1 min. This high-speed, persistent elongation of neurites was suppressed by inhibitors of both microtubule and actin polymerization, indicating that the thermosensitive dynamics of these cytoskeletons play crucial roles in this heat-induced neurite outgrowth. Furthermore, we showed that microheating induced the regrowth of injured neurites and the interconnection of neurites. These results demonstrate the efficacy of optical microheating methods for the construction of arbitrary neural networks.

Original languageEnglish
Article number16611
JournalScientific Reports
Volume5
DOIs
Publication statusPublished - 2015 Nov 16

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elongation
muscular function
high speed
laser beams
muscle cells
gene expression
neurons
inhibitors
rats
polymerization
actuators
microscopes
optics
heat
water
excitation
temperature

ASJC Scopus subject areas

  • General

Cite this

Oyama, K., Zeeb, V., Kawamura, Y., Arai, T., Gotoh, M., Itoh, H., ... Ishiwata, SI. (2015). Triggering of high-speed neurite outgrowth using an optical microheater. Scientific Reports, 5, [16611]. https://doi.org/10.1038/srep16611

Triggering of high-speed neurite outgrowth using an optical microheater. / Oyama, Kotaro; Zeeb, Vadim; Kawamura, Yuki; Arai, Tomomi; Gotoh, Mizuho; Itoh, Hideki; Itabashi, Takeshi; Suzuki, Madoka; Ishiwata, Shin'Ichi.

In: Scientific Reports, Vol. 5, 16611, 16.11.2015.

Research output: Contribution to journalArticle

Oyama, K, Zeeb, V, Kawamura, Y, Arai, T, Gotoh, M, Itoh, H, Itabashi, T, Suzuki, M & Ishiwata, SI 2015, 'Triggering of high-speed neurite outgrowth using an optical microheater', Scientific Reports, vol. 5, 16611. https://doi.org/10.1038/srep16611
Oyama K, Zeeb V, Kawamura Y, Arai T, Gotoh M, Itoh H et al. Triggering of high-speed neurite outgrowth using an optical microheater. Scientific Reports. 2015 Nov 16;5. 16611. https://doi.org/10.1038/srep16611
Oyama, Kotaro ; Zeeb, Vadim ; Kawamura, Yuki ; Arai, Tomomi ; Gotoh, Mizuho ; Itoh, Hideki ; Itabashi, Takeshi ; Suzuki, Madoka ; Ishiwata, Shin'Ichi. / Triggering of high-speed neurite outgrowth using an optical microheater. In: Scientific Reports. 2015 ; Vol. 5.
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