tRNA recognition by CCA-adding enzyme.

Yukihide Tomari, Tsutomu Suzuki, Takuya Ueda

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The CCA-adding enzyme [ATP (CTP): tRNA nucleotidyltransferase (EC. 2.7.7.25)] catalyzes the addition of the conserved CCA sequence to the 3'-terminus of tRNAs. It is known that CCA-adding enzymes from all three kingdoms recognize the elbow region of L-shape tRNA formed by the tertiary interaction between T-loop and D-loop. We here demonstrate that C. elegans mitochondrial CCA-adding enzyme has broad substrate specificity toward nematode mitochondrial tRNAs which lack entire T- or D-arms, by cloning and characterizing the recombinant protein.

Original languageEnglish
Pages (from-to)77-78
Number of pages2
JournalNucleic acids research. Supplement (2001)
Issue number2
Publication statusPublished - 2002 Jan 1
Externally publishedYes

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Transfer RNA
Conserved Sequence
Elbow
Substrate Specificity
Recombinant Proteins
Organism Cloning
Arm
tRNA nucleotidyltransferase

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Tomari, Y., Suzuki, T., & Ueda, T. (2002). tRNA recognition by CCA-adding enzyme. Nucleic acids research. Supplement (2001), (2), 77-78.

tRNA recognition by CCA-adding enzyme. / Tomari, Yukihide; Suzuki, Tsutomu; Ueda, Takuya.

In: Nucleic acids research. Supplement (2001), No. 2, 01.01.2002, p. 77-78.

Research output: Contribution to journalArticle

Tomari, Y, Suzuki, T & Ueda, T 2002, 'tRNA recognition by CCA-adding enzyme.', Nucleic acids research. Supplement (2001), no. 2, pp. 77-78.
Tomari, Yukihide ; Suzuki, Tsutomu ; Ueda, Takuya. / tRNA recognition by CCA-adding enzyme. In: Nucleic acids research. Supplement (2001). 2002 ; No. 2. pp. 77-78.
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