Abstract
The CCA-adding enzyme [ATP (CTP): tRNA nucleotidyltransferase (EC. 2.7.7.25)] catalyzes the addition of the conserved CCA sequence to the 3'-terminus of tRNAs. It is known that CCA-adding enzymes from all three kingdoms recognize the elbow region of L-shape tRNA formed by the tertiary interaction between T-loop and D-loop. We here demonstrate that C. elegans mitochondrial CCA-adding enzyme has broad substrate specificity toward nematode mitochondrial tRNAs which lack entire T- or D-arms, by cloning and characterizing the recombinant protein.
Original language | English |
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Pages (from-to) | 77-78 |
Number of pages | 2 |
Journal | Nucleic acids research. Supplement (2001) |
Issue number | 2 |
DOIs | |
Publication status | Published - 2002 |
ASJC Scopus subject areas
- Medicine(all)