Abstract
The CCA-adding enzyme [ATP (CTP): tRNA nucleotidyltransferase (EC. 2.7.7.25)] catalyzes the addition of the conserved CCA sequence to the 3'-terminus of tRNAs. It is known that CCA-adding enzymes from all three kingdoms recognize the elbow region of L-shape tRNA formed by the tertiary interaction between T-loop and D-loop. We here demonstrate that C. elegans mitochondrial CCA-adding enzyme has broad substrate specificity toward nematode mitochondrial tRNAs which lack entire T- or D-arms, by cloning and characterizing the recombinant protein.
| Original language | English |
|---|---|
| Pages (from-to) | 77-78 |
| Number of pages | 2 |
| Journal | Nucleic acids research. Supplement (2001) |
| Issue number | 2 |
| Publication status | Published - 2002 |
Fingerprint
ASJC Scopus subject areas
- Medicine(all)
Cite this
Tomari, Y., Suzuki, T., & Ueda, T. (2002). tRNA recognition by CCA-adding enzyme. Nucleic acids research. Supplement (2001), (2), 77-78.