The CCA-adding enzyme [ATP (CTP): tRNA nucleotidyltransferase (EC. 22.214.171.124)] catalyzes the addition of the conserved CCA sequence to the 3'-terminus of tRNAs. It is known that CCA-adding enzymes from all three kingdoms recognize the elbow region of L-shape tRNA formed by the tertiary interaction between T-loop and D-loop. We here demonstrate that C. elegans mitochondrial CCA-adding enzyme has broad substrate specificity toward nematode mitochondrial tRNAs which lack entire T- or D-arms, by cloning and characterizing the recombinant protein.
|Number of pages||2|
|Journal||Nucleic acids research. Supplement (2001)|
|Publication status||Published - 2002|
ASJC Scopus subject areas