Abstract
The CCA-adding enzyme [ATP (CTP): tRNA nucleotidyltransferase (EC. 2.7.7.25)] catalyzes the addition of the conserved CCA sequence to the 3'-terminus of tRNAs. It is known that CCA-adding enzymes from all three kingdoms recognize the elbow region of L-shape tRNA formed by the tertiary interaction between T-loop and D-loop. We here demonstrate that C. elegans mitochondrial CCA-adding enzyme has broad substrate specificity toward nematode mitochondrial tRNAs which lack entire T- or D-arms, by cloning and characterizing the recombinant protein.
| Original language | English |
|---|---|
| Pages (from-to) | 77-78 |
| Number of pages | 2 |
| Journal | Nucleic acids research. Supplement (2001) |
| Issue number | 2 |
| Publication status | Published - 2002 Jan 1 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Medicine(all)
Cite this
tRNA recognition by CCA-adding enzyme. / Tomari, Yukihide; Suzuki, Tsutomu; Ueda, Takuya.
In: Nucleic acids research. Supplement (2001), No. 2, 01.01.2002, p. 77-78.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - tRNA recognition by CCA-adding enzyme.
AU - Tomari, Yukihide
AU - Suzuki, Tsutomu
AU - Ueda, Takuya
PY - 2002/1/1
Y1 - 2002/1/1
N2 - The CCA-adding enzyme [ATP (CTP): tRNA nucleotidyltransferase (EC. 2.7.7.25)] catalyzes the addition of the conserved CCA sequence to the 3'-terminus of tRNAs. It is known that CCA-adding enzymes from all three kingdoms recognize the elbow region of L-shape tRNA formed by the tertiary interaction between T-loop and D-loop. We here demonstrate that C. elegans mitochondrial CCA-adding enzyme has broad substrate specificity toward nematode mitochondrial tRNAs which lack entire T- or D-arms, by cloning and characterizing the recombinant protein.
AB - The CCA-adding enzyme [ATP (CTP): tRNA nucleotidyltransferase (EC. 2.7.7.25)] catalyzes the addition of the conserved CCA sequence to the 3'-terminus of tRNAs. It is known that CCA-adding enzymes from all three kingdoms recognize the elbow region of L-shape tRNA formed by the tertiary interaction between T-loop and D-loop. We here demonstrate that C. elegans mitochondrial CCA-adding enzyme has broad substrate specificity toward nematode mitochondrial tRNAs which lack entire T- or D-arms, by cloning and characterizing the recombinant protein.
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UR - http://www.scopus.com/inward/citedby.url?scp=0041818183&partnerID=8YFLogxK
M3 - Article
C2 - 12903113
AN - SCOPUS:0041818183
SP - 77
EP - 78
JO - Nucleic acids research. Supplement (2001)
JF - Nucleic acids research. Supplement (2001)
IS - 2
ER -