Tropomyosin modulates pH dependence of isometric tension

Hideaki Fujita, Shin'ichi Ishiwata

    Research output: Contribution to journalArticle

    16 Citations (Scopus)

    Abstract

    We investigated the effect of pH on isometric tension in actin filament- reconstituted and thin filament-reconstituted bovine cardiac muscle fibers in the pH range of 6.0-7.4. Thin filament was reconstituted from purified G- actin with either bovine cardiac tropomyosin (Tm) or rabbit skeletal Tm in conjunction with cardiac or skeletal troponin (Tn). Results showed that isometric tension decreased linearly with a decrease in pH. The slope of the pH-tension relation, ΔF/ΔpH (Δrelative tension/Δunit pH), was 0.28 and 0.44 in control cardiac fibers and skeletal fibers, respectively. In actin filament-reconstituted fibers without regulatory proteins, ΔF/ΔpH was 0.62, namely larger than that in cardiac or skeletal fibers. When reconstituted with cardiac Tm-Tn complex (nTm), ΔF/ΔpH recovered to 0.32, close to the value obtained in control cardiac fibers. When reconstituted with skeletal nTm, ΔF/ΔpH recovered to 0.48, close to the value for control skeletal fibers. To determine whether Tm or Tn is responsible for the inhibitory effects of nTm on the tension decrease caused by reduced pH, thin filament was reconstituted with cardiac Tm and skeletal Tn, or with skeletal Tm and cardiac Tn. When cardiac Tm was used, pH dependence of isometric tension coincided with that of control cardiac fibers. When skeletal Tm was used, the pH dependence coincided with that of control skeletal fibers. Furthermore, closely similar results were obtained in fibers reconstituted with actin and either cardiac or skeletal Tm without Tn. These results demonstrate that Tm but not Tn modulates the pH dependence of active tension.

    Original languageEnglish
    Pages (from-to)1540-1546
    Number of pages7
    JournalBiophysical Journal
    Volume77
    Issue number3
    Publication statusPublished - 1999 Sep

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    Tropomyosin
    Troponin
    Actin Cytoskeleton
    Actins
    Myocardium

    ASJC Scopus subject areas

    • Biophysics

    Cite this

    Fujita, H., & Ishiwata, S. (1999). Tropomyosin modulates pH dependence of isometric tension. Biophysical Journal, 77(3), 1540-1546.

    Tropomyosin modulates pH dependence of isometric tension. / Fujita, Hideaki; Ishiwata, Shin'ichi.

    In: Biophysical Journal, Vol. 77, No. 3, 09.1999, p. 1540-1546.

    Research output: Contribution to journalArticle

    Fujita, H & Ishiwata, S 1999, 'Tropomyosin modulates pH dependence of isometric tension', Biophysical Journal, vol. 77, no. 3, pp. 1540-1546.
    Fujita H, Ishiwata S. Tropomyosin modulates pH dependence of isometric tension. Biophysical Journal. 1999 Sep;77(3):1540-1546.
    Fujita, Hideaki ; Ishiwata, Shin'ichi. / Tropomyosin modulates pH dependence of isometric tension. In: Biophysical Journal. 1999 ; Vol. 77, No. 3. pp. 1540-1546.
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