Tweezing the cofactor preference of gymnosperm pinene synthase

Miki Tashiro, Koyo Ono, Yuki Kimura, Shigeko Kawai-Noma, Kyoichi Saito, Daisuke Umeno

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

The cellular activities of gymnosperms monoterpene synthases are largely compromised due to their requirement for manganese, which is deficient in microbial cells. Through site-saturation mutagenesis of the residue adjacent to metal-binding glutamate, we found that pinene synthase is highly mutable at this position yet drastically alter their metal binding preference, thereby quickly improving the cellular performance in heterologous hosts.

Original languageEnglish
Pages (from-to)1058-1061
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume82
Issue number6
DOIs
Publication statusPublished - 2018
Externally publishedYes

Keywords

  • Metabolic engineering
  • Metal dependency
  • Protein engineering
  • Synthetic biology

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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