Two-dimensional analysis of proteins specific to the bacterial magnetic particle membrane from Magnetospirillum sp. AMB-1

Yoshiko Okamura; Haruko Takeyama; Tadashi Matsunaga

doi:10.1385/ABAB:84-86:1-9:441

Two-dimensional analysis of proteins specific to the bacterial magnetic particle membrane from Magnetospirillum sp. AMB-1

Yoshiko Okamura, Haruko Takeyama, Tadashi Matsunaga^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

43 Citations (Scopus)

Abstract

We report the identification of five proteins expressed specifically on the bacterial magnetic particle (BMP) membrane of Magnetospirillum sp. AMB-1. These proteins are major components of the BMP membrane. The molecular weights were determined to be 12.0, 16.0, 24.8, 35.6, and 66.2 kDa by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Of these five, the 16.0- kDa protein was the most abundant in the BMP membrane. Furthermore, the 16.0- kDa protein consisted of two components each of differing pI. The 35.6-kDa protein was the second most abundant protein of the five detected.

Original language	English
Pages (from-to)	441-446
Number of pages	6
Journal	Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
Volume	84-86
DOIs	https://doi.org/10.1385/ABAB:84-86:1-9:441
Publication status	Published - 2000 Jan 1
Externally published	Yes

Keywords

Bacterial magnetic particle
Bacterial magnetic particle-specific protein
Magnetic bacteria

ASJC Scopus subject areas

Biotechnology
Bioengineering
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology

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10.1385/ABAB:84-86:1-9:441

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Two-dimensional analysis of proteins specific to the bacterial magnetic particle membrane from Magnetospirillum sp. AMB-1. / Okamura, Yoshiko; Takeyama, Haruko; Matsunaga, Tadashi.

In: Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology, Vol. 84-86, 01.01.2000, p. 441-446.

Research output: Contribution to journal › Article › peer-review

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AB - We report the identification of five proteins expressed specifically on the bacterial magnetic particle (BMP) membrane of Magnetospirillum sp. AMB-1. These proteins are major components of the BMP membrane. The molecular weights were determined to be 12.0, 16.0, 24.8, 35.6, and 66.2 kDa by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Of these five, the 16.0- kDa protein was the most abundant in the BMP membrane. Furthermore, the 16.0- kDa protein consisted of two components each of differing pI. The 35.6-kDa protein was the second most abundant protein of the five detected.

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KW - Bacterial magnetic particle-specific protein

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Two-dimensional analysis of proteins specific to the bacterial magnetic particle membrane from Magnetospirillum sp. AMB-1

Abstract

Keywords

ASJC Scopus subject areas

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