Two groups of thermophilic amino acid aminotransferases exhibiting broad substrate specificities for the synthesis of phenylglycine derivatives

Daisuke Koma, Toshiya Sawai, Ryotaro Hara, Shigeaki Harayama, Kuniki Kino

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Thirty two thermophilic amino acid aminotransferases (AATs) were expressed in Escherichia coli as soluble and active proteins. Based on their primary structures, the 32 AATs were divided into four phylogenetic groups (classes I, II, IV, and V). The substrate specificities of these AATs were examined, and 12 AATs were found capable of synthesizing ring-substituted phenylglycine derivatives such as hydroxyl-, methoxy-, and fluorophenylglycines. Eleven out of the 12 AATs were enzymes belonging to two phylogenetic groups namely, one subgroup of the class I family and the class IV family. AATs in these two groups may thus be useful for the synthesis of a variety of ring-substituted phenylglycine derivatives.

Original languageEnglish
Pages (from-to)775-784
Number of pages10
JournalApplied Microbiology and Biotechnology
Volume79
Issue number5
DOIs
Publication statusPublished - 2008 Jul 1

Keywords

  • Aminotransferase
  • Nonproteinogenic amino acid
  • Phenylglycine
  • Thermophile
  • Unnatural amino acid

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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