Two isoforms of chicken melanopsins show blue light sensitivity

Masaki Torii, Daisuke Kojima, Toshiyuki Okano, Atsushi Nakamura, Akihisa Terakita, Yoshinori Shichida, Akimori Wada, Yoshitaka Fukada

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Melanopsin is a vertebrate non-visual opsin and functions as a circadian photoreceptor in mammalian retinas. Here we found the expression of two kinds of melanopsin genes in the chicken pineal gland and identified the presence of five isoforms derived from these two genes. Reconstitution of the recombinant proteins with 11-cis-retinal revealed that at least two of these melanopsin protein isoforms can function as blue-sensitive photopigments with absorption maxima at 476-484 nm. These values are consistent with maximal sensitivities of action spectra determined from the physiological and behavioral studies on mammalian melanopsins. The melanopsin isoforms found in this study may function as pineal circadian photoreceptors.

Original languageEnglish
Pages (from-to)5327-5331
Number of pages5
JournalFEBS Letters
Volume581
Issue number27
DOIs
Publication statusPublished - 2007 Nov 13
Externally publishedYes

Fingerprint

Photophobia
Photosensitivity
Chickens
Protein Isoforms
Genes
Retinaldehyde
Opsins
Pineal Gland
Recombinant Proteins
Vertebrates
Retina
melanopsin

Keywords

  • Melanopsin
  • OPN4
  • Pineal gland

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Torii, M., Kojima, D., Okano, T., Nakamura, A., Terakita, A., Shichida, Y., ... Fukada, Y. (2007). Two isoforms of chicken melanopsins show blue light sensitivity. FEBS Letters, 581(27), 5327-5331. https://doi.org/10.1016/j.febslet.2007.10.019

Two isoforms of chicken melanopsins show blue light sensitivity. / Torii, Masaki; Kojima, Daisuke; Okano, Toshiyuki; Nakamura, Atsushi; Terakita, Akihisa; Shichida, Yoshinori; Wada, Akimori; Fukada, Yoshitaka.

In: FEBS Letters, Vol. 581, No. 27, 13.11.2007, p. 5327-5331.

Research output: Contribution to journalArticle

Torii, M, Kojima, D, Okano, T, Nakamura, A, Terakita, A, Shichida, Y, Wada, A & Fukada, Y 2007, 'Two isoforms of chicken melanopsins show blue light sensitivity', FEBS Letters, vol. 581, no. 27, pp. 5327-5331. https://doi.org/10.1016/j.febslet.2007.10.019
Torii M, Kojima D, Okano T, Nakamura A, Terakita A, Shichida Y et al. Two isoforms of chicken melanopsins show blue light sensitivity. FEBS Letters. 2007 Nov 13;581(27):5327-5331. https://doi.org/10.1016/j.febslet.2007.10.019
Torii, Masaki ; Kojima, Daisuke ; Okano, Toshiyuki ; Nakamura, Atsushi ; Terakita, Akihisa ; Shichida, Yoshinori ; Wada, Akimori ; Fukada, Yoshitaka. / Two isoforms of chicken melanopsins show blue light sensitivity. In: FEBS Letters. 2007 ; Vol. 581, No. 27. pp. 5327-5331.
@article{e537978bd3a94b25b5e5911095666fa0,
title = "Two isoforms of chicken melanopsins show blue light sensitivity",
abstract = "Melanopsin is a vertebrate non-visual opsin and functions as a circadian photoreceptor in mammalian retinas. Here we found the expression of two kinds of melanopsin genes in the chicken pineal gland and identified the presence of five isoforms derived from these two genes. Reconstitution of the recombinant proteins with 11-cis-retinal revealed that at least two of these melanopsin protein isoforms can function as blue-sensitive photopigments with absorption maxima at 476-484 nm. These values are consistent with maximal sensitivities of action spectra determined from the physiological and behavioral studies on mammalian melanopsins. The melanopsin isoforms found in this study may function as pineal circadian photoreceptors.",
keywords = "Melanopsin, OPN4, Pineal gland",
author = "Masaki Torii and Daisuke Kojima and Toshiyuki Okano and Atsushi Nakamura and Akihisa Terakita and Yoshinori Shichida and Akimori Wada and Yoshitaka Fukada",
year = "2007",
month = "11",
day = "13",
doi = "10.1016/j.febslet.2007.10.019",
language = "English",
volume = "581",
pages = "5327--5331",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "27",

}

TY - JOUR

T1 - Two isoforms of chicken melanopsins show blue light sensitivity

AU - Torii, Masaki

AU - Kojima, Daisuke

AU - Okano, Toshiyuki

AU - Nakamura, Atsushi

AU - Terakita, Akihisa

AU - Shichida, Yoshinori

AU - Wada, Akimori

AU - Fukada, Yoshitaka

PY - 2007/11/13

Y1 - 2007/11/13

N2 - Melanopsin is a vertebrate non-visual opsin and functions as a circadian photoreceptor in mammalian retinas. Here we found the expression of two kinds of melanopsin genes in the chicken pineal gland and identified the presence of five isoforms derived from these two genes. Reconstitution of the recombinant proteins with 11-cis-retinal revealed that at least two of these melanopsin protein isoforms can function as blue-sensitive photopigments with absorption maxima at 476-484 nm. These values are consistent with maximal sensitivities of action spectra determined from the physiological and behavioral studies on mammalian melanopsins. The melanopsin isoforms found in this study may function as pineal circadian photoreceptors.

AB - Melanopsin is a vertebrate non-visual opsin and functions as a circadian photoreceptor in mammalian retinas. Here we found the expression of two kinds of melanopsin genes in the chicken pineal gland and identified the presence of five isoforms derived from these two genes. Reconstitution of the recombinant proteins with 11-cis-retinal revealed that at least two of these melanopsin protein isoforms can function as blue-sensitive photopigments with absorption maxima at 476-484 nm. These values are consistent with maximal sensitivities of action spectra determined from the physiological and behavioral studies on mammalian melanopsins. The melanopsin isoforms found in this study may function as pineal circadian photoreceptors.

KW - Melanopsin

KW - OPN4

KW - Pineal gland

UR - http://www.scopus.com/inward/record.url?scp=35648969577&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=35648969577&partnerID=8YFLogxK

U2 - 10.1016/j.febslet.2007.10.019

DO - 10.1016/j.febslet.2007.10.019

M3 - Article

VL - 581

SP - 5327

EP - 5331

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 27

ER -