Two isoforms of chicken melanopsins show blue light sensitivity

Masaki Torii; Daisuke Kojima; Toshiyuki Okano; Atsushi Nakamura; Akihisa Terakita; Yoshinori Shichida; Akimori Wada; Yoshitaka Fukada

doi:10.1016/j.febslet.2007.10.019

Two isoforms of chicken melanopsins show blue light sensitivity

Masaki Torii, Daisuke Kojima, Toshiyuki Okano, Atsushi Nakamura, Akihisa Terakita, Yoshinori Shichida, Akimori Wada, Yoshitaka Fukada^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

67 Citations (Scopus)

Abstract

Melanopsin is a vertebrate non-visual opsin and functions as a circadian photoreceptor in mammalian retinas. Here we found the expression of two kinds of melanopsin genes in the chicken pineal gland and identified the presence of five isoforms derived from these two genes. Reconstitution of the recombinant proteins with 11-cis-retinal revealed that at least two of these melanopsin protein isoforms can function as blue-sensitive photopigments with absorption maxima at 476-484 nm. These values are consistent with maximal sensitivities of action spectra determined from the physiological and behavioral studies on mammalian melanopsins. The melanopsin isoforms found in this study may function as pineal circadian photoreceptors.

Original language	English
Pages (from-to)	5327-5331
Number of pages	5
Journal	FEBS Letters
Volume	581
Issue number	27
DOIs	https://doi.org/10.1016/j.febslet.2007.10.019
Publication status	Published - 2007 Nov 13
Externally published	Yes

Keywords

Melanopsin
OPN4
Pineal gland

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2007.10.019

Cite this

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title = "Two isoforms of chicken melanopsins show blue light sensitivity",

abstract = "Melanopsin is a vertebrate non-visual opsin and functions as a circadian photoreceptor in mammalian retinas. Here we found the expression of two kinds of melanopsin genes in the chicken pineal gland and identified the presence of five isoforms derived from these two genes. Reconstitution of the recombinant proteins with 11-cis-retinal revealed that at least two of these melanopsin protein isoforms can function as blue-sensitive photopigments with absorption maxima at 476-484 nm. These values are consistent with maximal sensitivities of action spectra determined from the physiological and behavioral studies on mammalian melanopsins. The melanopsin isoforms found in this study may function as pineal circadian photoreceptors.",

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T1 - Two isoforms of chicken melanopsins show blue light sensitivity

AU - Torii, Masaki

AU - Kojima, Daisuke

AU - Okano, Toshiyuki

AU - Nakamura, Atsushi

AU - Terakita, Akihisa

AU - Shichida, Yoshinori

AU - Wada, Akimori

AU - Fukada, Yoshitaka

PY - 2007/11/13

Y1 - 2007/11/13

N2 - Melanopsin is a vertebrate non-visual opsin and functions as a circadian photoreceptor in mammalian retinas. Here we found the expression of two kinds of melanopsin genes in the chicken pineal gland and identified the presence of five isoforms derived from these two genes. Reconstitution of the recombinant proteins with 11-cis-retinal revealed that at least two of these melanopsin protein isoforms can function as blue-sensitive photopigments with absorption maxima at 476-484 nm. These values are consistent with maximal sensitivities of action spectra determined from the physiological and behavioral studies on mammalian melanopsins. The melanopsin isoforms found in this study may function as pineal circadian photoreceptors.

AB - Melanopsin is a vertebrate non-visual opsin and functions as a circadian photoreceptor in mammalian retinas. Here we found the expression of two kinds of melanopsin genes in the chicken pineal gland and identified the presence of five isoforms derived from these two genes. Reconstitution of the recombinant proteins with 11-cis-retinal revealed that at least two of these melanopsin protein isoforms can function as blue-sensitive photopigments with absorption maxima at 476-484 nm. These values are consistent with maximal sensitivities of action spectra determined from the physiological and behavioral studies on mammalian melanopsins. The melanopsin isoforms found in this study may function as pineal circadian photoreceptors.

KW - Melanopsin

KW - OPN4

KW - Pineal gland

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Two isoforms of chicken melanopsins show blue light sensitivity

Abstract

Keywords

ASJC Scopus subject areas

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