Two isoforms of FTZ-F1 messenger RNA

Molecular cloning and their expression in the frog testis

Takeshi Nakajima, Minoru Takase, Ikuo Miura, Masahisa Nakamura

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

FTZ-F1, a member of the orphan nuclear receptors, is a transcriptional factor regulating the expression of the fushi tarazu gene in Drosophila (Lavorgna et al., 1991. Science 252, 848-851). Previously, we cloned a frog homologue of FTZ-F1 (rrFTZ-F1α; GenBank Accession No. AB035498). In this study, we isolated the rrFTZ-F1β cDNA encoding a protein of 469 amino acids. Then, expressions of two types (α and β) of rrFTZ-F1 mRNAs were examined during development of embryos and gonads in the frog Rana rugosa. They were expressed in the embryo at stage 12. Expressions of both the α and β mRNAs became stronger in the testis of frogs at stage XXV and were most prominent in that of frogs 2 months after metamorphosis. In the former testis, spermatogonia were the only germ cells in the seminiferous tubules, whilst postmeiotic cells were observed in the latter testis. Expression of the typea mRNA was more prominent. In addition, we cloned the regions with either exon I or II of the rrFTZ-F1 gene. Genomic structure analysis revealed that rrFTZ-F1β is a partial exon I-truncated variant of rrFTZ-F1α. The results suggest that rrFTZ-F1α and -β are expressed from the same gene by alternative splicing and that they may play an important role(s) in differentiation of premeiotic germ cells in the testis of the frog R. rugosa. (C) 2000 Elsevier Science B.V. All rights reserved.

Original languageEnglish
Pages (from-to)203-212
Number of pages10
JournalGene
Volume248
Issue number1-2
DOIs
Publication statusPublished - 2000 May 2
Externally publishedYes

Fingerprint

Molecular Cloning
Anura
Testis
Protein Isoforms
Messenger RNA
Germ Cells
Exons
Orphan Nuclear Receptors
Genes
Ranidae
Spermatogonia
Seminiferous Tubules
Nucleic Acid Databases
Alternative Splicing
Gonads
Drosophila
Embryonic Development
Embryonic Structures
Complementary DNA
Amino Acids

Keywords

  • 5'/3' RACE
  • cDNA
  • Clonings
  • Frog
  • FTZ-F1 isoforms
  • Genomic DNA
  • RT-PCR
  • Testis

ASJC Scopus subject areas

  • Genetics

Cite this

Two isoforms of FTZ-F1 messenger RNA : Molecular cloning and their expression in the frog testis. / Nakajima, Takeshi; Takase, Minoru; Miura, Ikuo; Nakamura, Masahisa.

In: Gene, Vol. 248, No. 1-2, 02.05.2000, p. 203-212.

Research output: Contribution to journalArticle

Nakajima, Takeshi ; Takase, Minoru ; Miura, Ikuo ; Nakamura, Masahisa. / Two isoforms of FTZ-F1 messenger RNA : Molecular cloning and their expression in the frog testis. In: Gene. 2000 ; Vol. 248, No. 1-2. pp. 203-212.
@article{01ecd836031846ecb28cf3cd4cb81215,
title = "Two isoforms of FTZ-F1 messenger RNA: Molecular cloning and their expression in the frog testis",
abstract = "FTZ-F1, a member of the orphan nuclear receptors, is a transcriptional factor regulating the expression of the fushi tarazu gene in Drosophila (Lavorgna et al., 1991. Science 252, 848-851). Previously, we cloned a frog homologue of FTZ-F1 (rrFTZ-F1α; GenBank Accession No. AB035498). In this study, we isolated the rrFTZ-F1β cDNA encoding a protein of 469 amino acids. Then, expressions of two types (α and β) of rrFTZ-F1 mRNAs were examined during development of embryos and gonads in the frog Rana rugosa. They were expressed in the embryo at stage 12. Expressions of both the α and β mRNAs became stronger in the testis of frogs at stage XXV and were most prominent in that of frogs 2 months after metamorphosis. In the former testis, spermatogonia were the only germ cells in the seminiferous tubules, whilst postmeiotic cells were observed in the latter testis. Expression of the typea mRNA was more prominent. In addition, we cloned the regions with either exon I or II of the rrFTZ-F1 gene. Genomic structure analysis revealed that rrFTZ-F1β is a partial exon I-truncated variant of rrFTZ-F1α. The results suggest that rrFTZ-F1α and -β are expressed from the same gene by alternative splicing and that they may play an important role(s) in differentiation of premeiotic germ cells in the testis of the frog R. rugosa. (C) 2000 Elsevier Science B.V. All rights reserved.",
keywords = "5'/3' RACE, cDNA, Clonings, Frog, FTZ-F1 isoforms, Genomic DNA, RT-PCR, Testis",
author = "Takeshi Nakajima and Minoru Takase and Ikuo Miura and Masahisa Nakamura",
year = "2000",
month = "5",
day = "2",
doi = "10.1016/S0378-1119(00)00119-0",
language = "English",
volume = "248",
pages = "203--212",
journal = "Gene",
issn = "0378-1119",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Two isoforms of FTZ-F1 messenger RNA

T2 - Molecular cloning and their expression in the frog testis

AU - Nakajima, Takeshi

AU - Takase, Minoru

AU - Miura, Ikuo

AU - Nakamura, Masahisa

PY - 2000/5/2

Y1 - 2000/5/2

N2 - FTZ-F1, a member of the orphan nuclear receptors, is a transcriptional factor regulating the expression of the fushi tarazu gene in Drosophila (Lavorgna et al., 1991. Science 252, 848-851). Previously, we cloned a frog homologue of FTZ-F1 (rrFTZ-F1α; GenBank Accession No. AB035498). In this study, we isolated the rrFTZ-F1β cDNA encoding a protein of 469 amino acids. Then, expressions of two types (α and β) of rrFTZ-F1 mRNAs were examined during development of embryos and gonads in the frog Rana rugosa. They were expressed in the embryo at stage 12. Expressions of both the α and β mRNAs became stronger in the testis of frogs at stage XXV and were most prominent in that of frogs 2 months after metamorphosis. In the former testis, spermatogonia were the only germ cells in the seminiferous tubules, whilst postmeiotic cells were observed in the latter testis. Expression of the typea mRNA was more prominent. In addition, we cloned the regions with either exon I or II of the rrFTZ-F1 gene. Genomic structure analysis revealed that rrFTZ-F1β is a partial exon I-truncated variant of rrFTZ-F1α. The results suggest that rrFTZ-F1α and -β are expressed from the same gene by alternative splicing and that they may play an important role(s) in differentiation of premeiotic germ cells in the testis of the frog R. rugosa. (C) 2000 Elsevier Science B.V. All rights reserved.

AB - FTZ-F1, a member of the orphan nuclear receptors, is a transcriptional factor regulating the expression of the fushi tarazu gene in Drosophila (Lavorgna et al., 1991. Science 252, 848-851). Previously, we cloned a frog homologue of FTZ-F1 (rrFTZ-F1α; GenBank Accession No. AB035498). In this study, we isolated the rrFTZ-F1β cDNA encoding a protein of 469 amino acids. Then, expressions of two types (α and β) of rrFTZ-F1 mRNAs were examined during development of embryos and gonads in the frog Rana rugosa. They were expressed in the embryo at stage 12. Expressions of both the α and β mRNAs became stronger in the testis of frogs at stage XXV and were most prominent in that of frogs 2 months after metamorphosis. In the former testis, spermatogonia were the only germ cells in the seminiferous tubules, whilst postmeiotic cells were observed in the latter testis. Expression of the typea mRNA was more prominent. In addition, we cloned the regions with either exon I or II of the rrFTZ-F1 gene. Genomic structure analysis revealed that rrFTZ-F1β is a partial exon I-truncated variant of rrFTZ-F1α. The results suggest that rrFTZ-F1α and -β are expressed from the same gene by alternative splicing and that they may play an important role(s) in differentiation of premeiotic germ cells in the testis of the frog R. rugosa. (C) 2000 Elsevier Science B.V. All rights reserved.

KW - 5'/3' RACE

KW - cDNA

KW - Clonings

KW - Frog

KW - FTZ-F1 isoforms

KW - Genomic DNA

KW - RT-PCR

KW - Testis

UR - http://www.scopus.com/inward/record.url?scp=0034595237&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034595237&partnerID=8YFLogxK

U2 - 10.1016/S0378-1119(00)00119-0

DO - 10.1016/S0378-1119(00)00119-0

M3 - Article

VL - 248

SP - 203

EP - 212

JO - Gene

JF - Gene

SN - 0378-1119

IS - 1-2

ER -