Two KaiA-binding domains of cyanobacterial circadian clock protein KaiC

Yasuhito Taniguchi; Akihiro Yamaguchi; Atsushi Hijikata; Hideo Iwasaki; Kyoko Kamagata; Masahiro Ishiura; Mitiko Go; Takao Kondo

doi:10.1016/S0014-5793(01)02408-5

Two KaiA-binding domains of cyanobacterial circadian clock protein KaiC

Yasuhito Taniguchi, Akihiro Yamaguchi, Atsushi Hijikata, Hideo Iwasaki, Kyoko Kamagata, Masahiro Ishiura, Mitiko Go, Takao Kondo^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

53 Citations (Scopus)

Abstract

kaiABC, a gene cluster, encodes KaiA, KaiB and KaiC proteins that are essential to circadian rhythms in the unicellular cyanobacterium Synechococcus sp. strain PCC 7942. Kai proteins can interact with each other in all possible combinations. This study identified two KaiA-binding domains (C_KABD1 and C_KABD2) in KaiC at corresponding regions of its duplicated structure. Clock mutations on the two domains and kaiA altered the strength of C_KABD-KaiA interactions assayed by the yeast two-hybrid system. Thus, interaction between KaiA and KaiC through C_KABD1 and C_KABD2 is likely important for circadian timing in the cyanobacterium.

Original language	English
Pages (from-to)	86-90
Number of pages	5
Journal	FEBS Letters
Volume	496
Issue number	2-3
DOIs	https://doi.org/10.1016/S0014-5793(01)02408-5
Publication status	Published - 2001 May 11
Externally published	Yes

Keywords

Circadian rhythm
Cyanobacterium
Protein-protein interaction
Synechococcus
kai gene

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(01)02408-5

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title = "Two KaiA-binding domains of cyanobacterial circadian clock protein KaiC",

abstract = "kaiABC, a gene cluster, encodes KaiA, KaiB and KaiC proteins that are essential to circadian rhythms in the unicellular cyanobacterium Synechococcus sp. strain PCC 7942. Kai proteins can interact with each other in all possible combinations. This study identified two KaiA-binding domains (CKABD1 and CKABD2) in KaiC at corresponding regions of its duplicated structure. Clock mutations on the two domains and kaiA altered the strength of CKABD-KaiA interactions assayed by the yeast two-hybrid system. Thus, interaction between KaiA and KaiC through CKABD1 and CKABD2 is likely important for circadian timing in the cyanobacterium.",

keywords = "Circadian rhythm, Cyanobacterium, Protein-protein interaction, Synechococcus, kai gene",

author = "Yasuhito Taniguchi and Akihiro Yamaguchi and Atsushi Hijikata and Hideo Iwasaki and Kyoko Kamagata and Masahiro Ishiura and Mitiko Go and Takao Kondo",

note = "Funding Information: This research was supported by grants from the Japanese Ministry of Education, Science and Culture (11233203, 11440234, 11558089, 11694199 to T.K. and M.I. and 12207002, 12208006, 1559007, 11480189 to M.G.).",

year = "2001",

month = may,

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doi = "10.1016/S0014-5793(01)02408-5",

language = "English",

volume = "496",

pages = "86--90",

journal = "FEBS Letters",

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T1 - Two KaiA-binding domains of cyanobacterial circadian clock protein KaiC

AU - Taniguchi, Yasuhito

AU - Yamaguchi, Akihiro

AU - Hijikata, Atsushi

AU - Iwasaki, Hideo

AU - Kamagata, Kyoko

AU - Ishiura, Masahiro

AU - Go, Mitiko

AU - Kondo, Takao

N1 - Funding Information: This research was supported by grants from the Japanese Ministry of Education, Science and Culture (11233203, 11440234, 11558089, 11694199 to T.K. and M.I. and 12207002, 12208006, 1559007, 11480189 to M.G.).

PY - 2001/5/11

Y1 - 2001/5/11

N2 - kaiABC, a gene cluster, encodes KaiA, KaiB and KaiC proteins that are essential to circadian rhythms in the unicellular cyanobacterium Synechococcus sp. strain PCC 7942. Kai proteins can interact with each other in all possible combinations. This study identified two KaiA-binding domains (CKABD1 and CKABD2) in KaiC at corresponding regions of its duplicated structure. Clock mutations on the two domains and kaiA altered the strength of CKABD-KaiA interactions assayed by the yeast two-hybrid system. Thus, interaction between KaiA and KaiC through CKABD1 and CKABD2 is likely important for circadian timing in the cyanobacterium.

AB - kaiABC, a gene cluster, encodes KaiA, KaiB and KaiC proteins that are essential to circadian rhythms in the unicellular cyanobacterium Synechococcus sp. strain PCC 7942. Kai proteins can interact with each other in all possible combinations. This study identified two KaiA-binding domains (CKABD1 and CKABD2) in KaiC at corresponding regions of its duplicated structure. Clock mutations on the two domains and kaiA altered the strength of CKABD-KaiA interactions assayed by the yeast two-hybrid system. Thus, interaction between KaiA and KaiC through CKABD1 and CKABD2 is likely important for circadian timing in the cyanobacterium.

KW - Circadian rhythm

KW - Cyanobacterium

KW - Protein-protein interaction

KW - Synechococcus

KW - kai gene

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AN - SCOPUS:0035843996

SN - 0014-5793

VL - 496

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EP - 90

JO - FEBS Letters

JF - FEBS Letters

IS - 2-3

ER -

Two KaiA-binding domains of cyanobacterial circadian clock protein KaiC

Abstract

Keywords

ASJC Scopus subject areas

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