Two KaiA-binding domains of cyanobacterial circadian clock protein KaiC

Yasuhito Taniguchi, Akihiro Yamaguchi, Atsushi Hijikata, Hideo Iwasaki, Kyoko Kamagata, Masahiro Ishiura, Mitiko Go, Takao Kondo

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

kaiABC, a gene cluster, encodes KaiA, KaiB and KaiC proteins that are essential to circadian rhythms in the unicellular cyanobacterium Synechococcus sp. strain PCC 7942. Kai proteins can interact with each other in all possible combinations. This study identified two KaiA-binding domains (CKABD1 and CKABD2) in KaiC at corresponding regions of its duplicated structure. Clock mutations on the two domains and kaiA altered the strength of CKABD-KaiA interactions assayed by the yeast two-hybrid system. Thus, interaction between KaiA and KaiC through CKABD1 and CKABD2 is likely important for circadian timing in the cyanobacterium.

Original languageEnglish
Pages (from-to)86-90
Number of pages5
JournalFEBS Letters
Volume496
Issue number2-3
DOIs
Publication statusPublished - 2001 May 11
Externally publishedYes

Fingerprint

Circadian Clocks
Cyanobacteria
Clocks
Synechococcus
Two-Hybrid System Techniques
Multigene Family
Circadian Rhythm
Hybrid systems
Yeast
Proteins
Genes
Mutation

Keywords

  • Circadian rhythm
  • Cyanobacterium
  • kai gene
  • Protein-protein interaction
  • Synechococcus

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Taniguchi, Y., Yamaguchi, A., Hijikata, A., Iwasaki, H., Kamagata, K., Ishiura, M., ... Kondo, T. (2001). Two KaiA-binding domains of cyanobacterial circadian clock protein KaiC. FEBS Letters, 496(2-3), 86-90. https://doi.org/10.1016/S0014-5793(01)02408-5

Two KaiA-binding domains of cyanobacterial circadian clock protein KaiC. / Taniguchi, Yasuhito; Yamaguchi, Akihiro; Hijikata, Atsushi; Iwasaki, Hideo; Kamagata, Kyoko; Ishiura, Masahiro; Go, Mitiko; Kondo, Takao.

In: FEBS Letters, Vol. 496, No. 2-3, 11.05.2001, p. 86-90.

Research output: Contribution to journalArticle

Taniguchi, Y, Yamaguchi, A, Hijikata, A, Iwasaki, H, Kamagata, K, Ishiura, M, Go, M & Kondo, T 2001, 'Two KaiA-binding domains of cyanobacterial circadian clock protein KaiC', FEBS Letters, vol. 496, no. 2-3, pp. 86-90. https://doi.org/10.1016/S0014-5793(01)02408-5
Taniguchi Y, Yamaguchi A, Hijikata A, Iwasaki H, Kamagata K, Ishiura M et al. Two KaiA-binding domains of cyanobacterial circadian clock protein KaiC. FEBS Letters. 2001 May 11;496(2-3):86-90. https://doi.org/10.1016/S0014-5793(01)02408-5
Taniguchi, Yasuhito ; Yamaguchi, Akihiro ; Hijikata, Atsushi ; Iwasaki, Hideo ; Kamagata, Kyoko ; Ishiura, Masahiro ; Go, Mitiko ; Kondo, Takao. / Two KaiA-binding domains of cyanobacterial circadian clock protein KaiC. In: FEBS Letters. 2001 ; Vol. 496, No. 2-3. pp. 86-90.
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